FastQCFastQC Report
Wed 25 May 2016
SRR2074768_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074768_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3650138
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG4550.033.82363540
CGTATGC4750.030.69424641
TATGCCG4600.030.52125443
CTCGTAT5150.028.31022839
TCTCGTA6400.024.46835938
CGAGAGT7200.022.87463628
CGAGACG7100.022.81716223
ACGAGAG7700.022.79184327
ATCTCGT6650.022.3304737
GACGAGA8350.021.0176326
ACGAGAC8250.020.2911422
GGTATCA96650.020.0872081
ATGCCGT7100.019.39405644
ATTGGCG4050.019.33381821
CCCACGA7850.018.57371319
GAGACGA9450.018.28592324
TGCCGTC7550.018.2381245
ATTTACG4450.017.59546554
GTATCAA140950.017.4328671
GCCGTCT7400.017.14837546
Produced by FastQC (version 0.11.5)