FastQCFastQC Report
Wed 25 May 2016
SRR2074767_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074767_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3943211
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG7300.030.32824739
TATGCCG6250.029.80749143
GTAGCGT7400.029.5535535
AGCGTCG7900.028.02483637
TAGCGTC7950.027.84858136
AATCTCG6800.027.7936436
CTCGTAT6600.027.4086239
CGTGGTA7800.027.34568442
GCGTCGT8200.027.328838
AGTAGCG8400.026.35669134
ACGTTCA7500.026.2795527
CCGTCTT6900.024.65175247
TGCCGTC8100.024.33291645
TCTCGTA7850.023.38813438
ATGCCGT7850.023.38813444
TCGTGGT9700.022.82435241
TCGTATG8300.022.77069540
GCCGTCT8100.022.66627746
GGTATCA88100.022.5566581
GGTATTC8100.022.3329545
Produced by FastQC (version 0.11.5)