FastQCFastQC Report
Wed 25 May 2016
SRR2074766_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074766_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2207374
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC27500.12458242237156004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT6050.041.05695338
CGTGGTA6500.040.70680642
AGCGTCG6250.040.60711337
GTAGCGT6200.040.49911535
TAGCGTC6200.040.0636436
CGTCGTG6350.039.96763639
ATTGGCG3950.036.91142721
TAGCGGA4250.036.84625634
ATTTACG4300.035.79072654
GGTAGCG4850.032.84464632
AGTAGCG7900.032.46764834
TATGCCG3500.031.6278843
TCGTGGT8400.030.21362541
TCGTATG4200.030.21362540
GGTATTC6100.030.09838745
TATCTCG5300.029.03710236
TCTCGTA4850.028.94782338
GGGTAGT8750.028.07938624
CTCGTAT4750.027.28361339
AGCGGAT6050.027.22254635
Produced by FastQC (version 0.11.5)