FastQCFastQC Report
Wed 25 May 2016
SRR2074765_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074765_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904406
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT452.0838525E-741.99875337
TCGACGG1950.038.76807829
CTCGACG2200.034.36261728
ACTCGAC2150.032.65019227
CGACGGC2100.032.14190330
TCGCGTA601.9814615E-631.49906338
TAGCCCG350.0044824930.8596385
CGTCGTG1251.8189894E-1228.07916639
ATAACGC706.5755703E-627.0021823
CGAGACT2900.026.06819223
AGCGTCG1501.8189894E-1225.1992537
CGGCATA651.233463E-424.92233733
GACTCGA2850.024.63084626
ACGGCAT2650.023.43326832
AGTAGCG1750.023.1421734
CATCGCG702.0389617E-423.14216836
TAACGCA852.9589291E-522.2370934
GACGGCA3050.022.13049131
GCGTCGT1607.094059E-1121.9368538
GTAGCGT1751.0913936E-1121.5993635
Produced by FastQC (version 0.11.5)