FastQCFastQC Report
Wed 25 May 2016
SRR2074764_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074764_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145967
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC26310.1226020717000774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG6400.042.60814337
CGTCGTG6400.041.7644239
TAGCGTC6950.038.84779736
GTAGCGT7050.038.29676435
CGTGGTA7150.037.76114742
TATGCCG4300.037.6733343
GCGTCGT7100.037.64679738
AGTAGCG7500.035.9989634
ATTGGCG2450.034.1630721
TCGTATG5150.034.07668340
CTCGTAT5350.033.81210739
TCGTGGT8100.033.3323741
TAGCGGA2400.032.62405834
GGGTAGT8450.031.95173824
GTCGTGG8500.031.76378840
GGTATTC6750.031.59908745
TCTCGTA5650.031.53891238
CCGTCTT5000.031.31909647
ATTTACG2750.029.45369554
CGTATGC6050.029.00742541
Produced by FastQC (version 0.11.5)