FastQCFastQC Report
Wed 25 May 2016
SRR2074763_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074763_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008223
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2550.037.05822843
TCGTATG3200.032.9057240
ATTTACG3750.032.3994854
CGTATGC3000.031.4994941
TAGCGGA4250.030.49362834
AGCGGAT4500.028.79953635
ATGCCGT3200.028.68703844
ATTGGCG4000.027.6759321
TCTCGTA3950.026.65779738
GGTAGCG4950.026.18139532
ATCTCGT4050.025.9995837
ACGAGAC6300.025.7145122
CGGATGA5300.025.47128737
TGCCGTC3300.025.36322845
CTCGTAT4350.024.20650539
GCGGATG5900.023.33860636
CCGACCC3050.023.0171684
TCCGACC2950.022.8821283
GTAGCGG6000.022.04964433
TCTGTCG4200.021.856798
Produced by FastQC (version 0.11.5)