FastQCFastQC Report
Wed 25 May 2016
SRR2074762_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074762_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868238
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC23950.12819565815490316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT5100.041.82228538
GTAGCGT5650.039.6625435
TAGCGTC5700.039.3146236
CGTGGTA5600.038.08917242
CGTCGTG5750.037.56510539
AGCGTCG6100.036.7366137
AGTAGCG6250.036.7189134
GGTATTC5200.033.749945
TATGCCG4100.033.58526643
CTCGTAT4350.032.27576439
GTCGTGG7050.031.40416340
TAGCGGA2850.031.26222834
TCGTGGT6850.031.13859441
ATTGGCG3000.030.59908921
TCGTATG4800.030.3749140
GGGTAGT7700.029.8043124
TCTCGTA4650.029.61202238
ATTTACG3200.029.5303754
TCGCTAT5000.028.6191529
GTGTAAG8250.027.16355512
Produced by FastQC (version 0.11.5)