FastQCFastQC Report
Wed 25 May 2016
SRR2074760_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074760_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1996304
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3500.036.2564943
GTAGCGT3550.033.46418435
TAGCGTC3700.032.10752536
AGCGTCG3900.030.46098937
CGTCGTG3800.029.84156439
TCGTATG3900.029.76869440
TCTCGTA3800.029.1310538
GCGTCGT4050.028.6661538
TACCGCT4750.028.42053829
TAGCGGA2300.026.99951234
ATGCCGT4900.026.99951244
CGTGGTA4400.026.9995142
CTCGTAT4400.026.9995139
AGTAGCG4650.026.70919434
CGTATGC4750.026.14689441
CCGTCTT4650.025.54792447
CTACCGC5500.025.03591228
ATTTACG2200.024.5450154
GGTAGCG2650.024.45238732
GACACGA4550.024.3292337
Produced by FastQC (version 0.11.5)