FastQCFastQC Report
Wed 25 May 2016
SRR2074759_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074759_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036398
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC41450.20354567230963688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG9400.043.65865739
CGTGGTA9700.041.75170542
GTAGCGT10000.041.57913235
AGCGTCG10050.041.3722737
GCGTCGT10200.040.7638538
TAGCGTC10350.039.3904836
AGTAGCG10750.038.92941734
GGTATTC9600.036.2804945
TCGTGGT11600.035.3785741
GTCGTGG11800.035.2365540
GTATTCC10350.033.6514746
GTGTAAG12600.032.7858312
CTGGGTA12850.032.7783322
GGGTAGT13250.031.99178324
TAAGCAT13600.031.76482415
AAGCATC13650.031.64924216
TATGCCG2450.030.85649743
ATCTGGG14500.029.98015620
GGTAGTC14100.029.87171625
GTAAGCA14400.029.81260914
Produced by FastQC (version 0.11.5)