FastQCFastQC Report
Wed 25 May 2016
SRR2074758_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074758_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814651
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2400.034.8739943
TCGTATG2400.032.62405440
CGTATGC3050.027.44182841
ATTTACG3850.026.64905254
GGTATCA42300.023.810361
ATTGGCG4000.023.62473721
ATGCCGT3650.022.93084344
CGCGTAT952.7175483E-622.73779914
TCTCGTA3500.022.37077938
GTATCAA69650.022.330521
TAGCGGA4650.022.06387734
AGCGGAT5400.020.99939235
CTCGTAT3800.020.60466639
ACGAGAC5050.019.78160522
GGTAGCG5400.018.99944932
CCGATCG1151.4193945E-518.783414
ATCAACG88050.018.4614013
TCAACGC87850.018.4419564
CAACGCA93050.018.3115835
ATCTCGT4150.018.2163437
Produced by FastQC (version 0.11.5)