FastQCFastQC Report
Wed 25 May 2016
SRR2074757_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074757_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2148417
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT4400.038.65792538
AGCGTCG4900.035.81524737
GTAGCGT4700.035.61594835
CGTCGTG5000.035.0989439
CGTGGTA5150.034.600942
TAGCGTC4850.034.5144236
ATTTACG2950.032.0329354
ATTGGCG3700.030.64772221
TCGTGGT5750.030.0512741
AGTAGCG5950.029.94867734
TAGCGGA3450.029.73823434
TATGCCG3150.029.1419843
GGTAGCG3750.028.07915332
GGGTAGT6900.025.43401524
TCGTATG3000.025.1992440
GGTATTC5100.024.88160345
GTCGTGG7400.024.81006240
GCGTGAA4600.024.6514325
ATCGCGT550.001365609124.54471437
CGTATGC3450.023.47755241
Produced by FastQC (version 0.11.5)