FastQCFastQC Report
Wed 25 May 2016
SRR2074756_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074756_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1996799
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC25760.12900647486301825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTC5850.041.5375836
GTAGCGT5850.041.5375835
CGTCGTG5900.041.1855639
GCGTCGT6400.039.2335438
CGTGGTA6000.038.69917742
AGCGTCG6500.038.2145737
AGTAGCG6500.038.2145734
TATGCCG3500.035.48495543
GTCGTGG7400.032.8371440
GGTATTC5700.031.7369645
CTCGTAT3950.031.4423739
TCGTGGT7550.031.1119241
GGGTAGT8350.030.0727224
GTGTAAG8450.029.39729512
CTGGGTA9350.028.29957222
GCCGTCT4450.027.91021546
ATGCCGT4400.027.61373944
TCTCGTA4600.026.99942438
TCGTATG4900.026.99942440
GGTGTAA9350.026.85638611
Produced by FastQC (version 0.11.5)