FastQCFastQC Report
Wed 25 May 2016
SRR2074755_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074755_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166613
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC28890.13334176431139294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT6700.039.4918335
CGTCGTG6900.038.73843839
TAGCGTC6950.037.68277736
GCGTCGT7550.035.4033438
AGCGTCG7650.035.29348437
AGTAGCG7700.035.0643134
CGTGGTA7650.034.58761242
TATGCCG3050.034.52396843
TCGTGGT8250.032.7266941
GGTATTC6950.031.4670645
GGGTAGT9150.029.50834724
GTCGTGG9450.029.14233640
CTCGTAT3950.028.70834539
AATCTCG4100.028.31656536
TCTCGTA3850.028.05144538
GGTGTAA10400.026.48029311
TGGTGTA10450.026.09522410
GTATTCC8350.025.867846
ATGCCGT4100.025.68246744
GTAAGCA10950.025.15023414
Produced by FastQC (version 0.11.5)