FastQCFastQC Report
Wed 25 May 2016
SRR2074754_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074754_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2712475
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC28300.1043327588272703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCG7900.040.6696534
CGTCGTG8000.039.1488139
GTAGCGT8200.038.19396235
TAGCGTC8250.037.30795736
AGCGTCG8600.037.04538737
GCGTCGT8550.036.63046638
CGTGGTA8750.036.1017642
TCGTGGT9700.033.12270741
GGTATTC8250.033.0547645
TATCTCG4200.031.49904436
GTCGTGG10850.030.35852640
GGGTAGT11950.027.33858324
ATCGACG806.0857565E-726.99967621
CTCGTAT4800.026.99918239
TATGCCG4250.026.68154343
GTGTAAG12650.026.03873412
GTATTCC10350.025.56538446
TGAGTAG13650.024.32893232
GGTGTAA13700.024.24013911
GGTAGTC13150.024.22740225
Produced by FastQC (version 0.11.5)