FastQCFastQC Report
Wed 25 May 2016
SRR2074753_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074753_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664477
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACG150.006218148453.9994727
TATGCCG900.044.9995643
GCGTCGT1500.041.39959738
TAGCGTC1400.040.49960336
TATAACG755.456968E-1239.5996172
TCGACGG2200.038.04508629
ATTGGCG504.7587673E-737.7996321
AGCGTCG1650.037.63599837
GTAGCGT1550.036.58028835
CGACGGC2300.036.39094530
GTCGTAT300.00210657135.9996545
AGTAGCG1750.035.48536734
CGTCGTG1800.034.49966439
CTCGACG2450.034.1629328
CGATATT402.1171373E-433.74967211
CGTGGTA1950.033.23044642
GGGTAGT2200.033.1360424
GACGGCA2450.033.060931
GGTATTC1650.032.72695545
ACGGCAT2150.032.65084532
Produced by FastQC (version 0.11.5)