FastQCFastQC Report
Wed 25 May 2016
SRR2074751_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074751_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2384476
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC31160.13067860611723497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG6300.041.57078639
CGTGGTA6650.039.38284742
GTAGCGT6850.039.0212935
TAGCGTC6900.038.7385336
GCGTCGT7050.037.5313338
AGCGTCG7500.036.71943337
AGTAGCG8100.034.332834
TCGTGGT8100.033.33281741
GTCGTGG8400.031.17808540
GGTATCA51500.028.1028941
GGGTAGT9600.027.5620724
GGTATTC7450.027.18078645
TATGCCG3500.026.99958243
CTCGTAT3750.026.63958739
GAGTAGC10450.026.0952933
GTGTAAG10750.025.86990712
GGTGTAA10900.025.76160811
GTATTCC8350.024.57446946
TGAGTAG11450.024.05202932
TGGGTAG11400.023.9206823
Produced by FastQC (version 0.11.5)