FastQCFastQC Report
Wed 25 May 2016
SRR2074750_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074750_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047622
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23330.11393704502100485No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21850.10670914846587895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3300.035.99952743
CTCGTAT4150.030.57791339
TCGCTAT4400.029.4541629
CGTATGC4250.029.22314641
TCGTATG4450.029.12321340
GCGTATC507.82825E-427.0009631
GTACGTT1005.2532414E-927.00030311
CCGTCTT3700.026.99964547
CGCTATG5200.026.48042330
TTACGTA1058.8348315E-925.7139545
ATGCCGT4250.025.41143244
TCTCGTA4800.024.74967438
CTCGCTA5600.024.58896428
TTGTACG1101.4488251E-824.5457329
GGTATCA49450.024.352741
GCCGTCT4350.024.2065846
CGTATGA1152.3228495E-823.47852720
GTACCGC2200.022.0906230
ACTCGCT5950.020.87367627
TGCCGTC5450.020.80706845
Produced by FastQC (version 0.11.5)