FastQCFastQC Report
Wed 25 May 2016
SRR2074749_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074749_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2795650
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG7550.034.3304240
TATGCCG6900.033.26018543
CGTATGC7450.032.97918741
CTCGTAT7800.031.845539
TCTCGTA8000.031.04936238
ATGCCGT8350.027.8083144
GGTATCA61300.024.7117671
GCCGTCT7350.024.61217946
CGAGACT12800.024.46824823
TGCCGTC9250.023.93507245
GTTATAC12800.023.83544730
ACGAGAC13100.023.70180322
CATCTCG10650.023.5769836
CGTATAG2300.022.3062821
GTAGCGT2200.022.09045635
CCGTCTT7900.021.87335847
TAGCGTC2250.021.59955836
GCGTCGT2500.021.59955638
CACGAGA14150.021.5617821
ATCTCGT11400.021.55218937
Produced by FastQC (version 0.11.5)