FastQCFastQC Report
Wed 25 May 2016
SRR2074748_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074748_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045865
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28980.14165157525056637No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24030.11745643040963114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3950.035.543943
TCGTATG4800.030.9371540
CGTATGC4950.028.36331641
CCGTCTT4500.027.60036347
CTCGTAT4950.027.27241939
ACTACCG6050.026.77655827
CGCTGAT4950.026.7269732
ATGCCGT5350.026.24333644
CGAGACT6250.025.4877123
TCTCGTA5100.025.41147638
GCCGTCT5050.025.12904246
TGCCGTC5700.024.631945
CTACCGC6550.024.32033528
TACCGCT6200.023.95134229
ACGAGAC8100.021.66642222
GGTATCA52750.021.1396161
TATACCG1156.0203274E-721.1307135
GTATGCC6950.020.58969542
CGTCGTG1451.1983502E-820.48252739
GTATCAA80900.020.3587341
Produced by FastQC (version 0.11.5)