FastQCFastQC Report
Wed 25 May 2016
SRR2074747_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074747_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2070487
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3450.033.6516343
ATTTACG2350.033.3186154
ATTGGCG2200.033.1358321
CGTATGC3400.031.76419641
ATCGCGT653.7005375E-629.07645437
TCGTATG3550.028.1403940
ACGAGAC8300.026.99956722
CGTAACG607.1619084E-526.99956548
TAGCGGA2850.026.5258934
GGTAGCG3000.026.09957932
TCTCGTA3950.025.97426438
GGTATCA50350.025.6870231
ATGCCGT4800.025.31209244
CGCGTAT651.2341002E-424.92267639
TTGGCGT3050.023.90125522
CTCGTAT4050.023.33295839
ATAACGC2100.023.1436023
TATGACG1303.4015102E-922.84578543
CACGAGA9700.022.82437521
TAACGCA2200.022.091624
Produced by FastQC (version 0.11.5)