FastQCFastQC Report
Wed 25 May 2016
SRR2074745_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074745_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983948
Sequences flagged as poor quality0
Sequence length60
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC29120.14677804055348223No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23430.11809785337115691No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21060.10615197575742912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG7200.043.1231939
GTAGCGT7600.042.62978735
CGTGGTA7450.041.676142
AGTAGCG7800.041.1905834
TAGCGTC8000.040.498336
AGCGTCG8050.039.2405937
GCGTCGT8350.037.18406738
GGTATTC6800.036.13083645
TCGTGGT8850.035.69341741
GGGTAGT9650.033.5754124
GTCGTGG9500.033.53543540
GTATACG1005.456968E-1232.405173
GTATTCC7350.031.95784246
GGTAGTC10650.030.4227925
CTGGGTA10900.029.72502122
GTGTAAG10950.029.34345412
TATGCCG4050.029.33210243
CTCGTAT4200.028.92735939
TGGTGTA11300.028.91247410
GTAGTCT11150.028.5735126
Produced by FastQC (version 0.11.5)