FastQCFastQC Report
Wed 25 May 2016
SRR2074744_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074744_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1867198
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC30840.1651672720300686No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19830.10620191324112387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT7350.041.50903338
CGTGGTA7300.039.94407742
CGTCGTG7750.039.3666339
TAGCGTC7850.039.20908436
AGCGTCG7900.038.96092637
GTAGCGT8150.037.76580435
AGTAGCG8400.037.2846634
TAGCGGA2750.036.3262534
GGGTAGT8500.035.893124
TCGTGGT8450.035.78597341
GGTATTC6750.033.99994745
GGTGTAA10000.032.1299511
GTCGTGG9750.031.84525340
ATTGGCG3000.031.49995421
CTGGGTA10000.030.77995322
TGGTGTA10100.030.74252910
GGTAGTC10050.030.62625
TAAGCAT10200.030.44113215
GGTAGCG3300.030.27187232
GTGTAAG10600.030.0565612
Produced by FastQC (version 0.11.5)