FastQCFastQC Report
Wed 25 May 2016
SRR2074742_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074742_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4613321
Sequences flagged as poor quality0
Sequence length60
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52340.11345406053469939No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48000.10404652093361809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5750.034.27772543
CTCGTAT6450.030.9762639
TATCTCG8150.029.484236
TCTCGTA7800.028.38417238
ATCTCGT9200.026.11915637
GACCCTA11500.023.00833526
ATGCCGT8850.022.88124744
CGAGACC11350.022.83664923
CCACGAG13700.020.89018420
TGCCGTC8750.020.67418745
CACGAGA13100.020.4042621
GGTATCA174750.020.396521
CCGTCTT7300.020.34236747
TCGTATG11200.018.80327840
GCCGTCT8400.018.3213446
GTATCAA255400.017.6561071
GAATATC17050.017.57743633
ATATCTC14150.017.55449535
CCCTAGA16600.017.24069428
CCCACGA16500.016.3633819
Produced by FastQC (version 0.11.5)