FastQCFastQC Report
Wed 25 May 2016
SRR2074741_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074741_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514400
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG1750.033.94219229
CTCGACG1900.031.26254728
GTAGCGT2150.028.88315635
TAGCGTC2150.027.62736736
CGTCGTG2250.027.5994639
TATGCCG706.5845434E-626.99947443
AGTAGCG2400.025.87449334
CGACGGC2200.025.77222330
GGTATCA19400.024.0768491
CGTGGTA2500.023.75953542
ACCGGTC801.8544364E-523.62453721
AGCGTCG2550.023.29366337
CGAGACT2600.022.84570723
CGATCGT852.962927E-522.2348625
GCGTCGT2800.022.17813938
CGAACCG753.2523987E-421.6002918
GACTCGA2500.021.59957726
AGACTCG3050.020.36025825
GTATCAA29800.019.4794851
ACTCGAC2650.019.35811227
Produced by FastQC (version 0.11.5)