FastQCFastQC Report
Wed 25 May 2016
SRR2074740_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074740_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2546396
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC30020.11789211104635729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG7700.042.42747539
AGCGTCG8200.040.16969737
CGTGGTA8250.039.5989842
GTAGCGT8050.039.5766235
TAGCGTC8150.039.09101536
GCGTCGT8400.038.89185338
AGTAGCG8750.036.4104934
TCGTGGT9400.035.0416541
TATGCCG3650.033.2868143
GGGTAGT10200.032.55798324
GTCGTGG10350.032.3469940
TAGCGGA2700.030.99920334
ATTTACG2250.030.00040254
ATTGGCG2700.028.99925421
GGTATTC8750.028.69752745
GTGTAAG12150.027.33316612
GGTGTAA12350.027.32776611
TCGTATG4450.027.30266640
GGTAGTC12500.026.99930625
TGGGTAG12450.026.89087323
Produced by FastQC (version 0.11.5)