FastQCFastQC Report
Wed 25 May 2016
SRR2074739_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074739_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1924412
Sequences flagged as poor quality0
Sequence length60
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC21160.10995566437956113No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG5400.037.99976339
GTAGCGT5800.036.7756335
CGTGGTA5750.036.15629642
TAGCGTC6050.034.3634236
AGTAGCG6300.034.28550334
AGCGTCG6000.034.19978737
GCGTCGT6150.033.36564638
GGTATTC5650.032.4953745
TCGTGGT6950.029.52499441
GGGTAGT7950.026.8300224
TAAGCAT8050.026.49672915
GTCGTGG7850.026.4839140
GTATTCC7200.025.87483846
GTGTAAG8550.025.26299912
GTAAGCA8950.024.43560214
GTGGTGT9050.024.1655969
GAGTAGC8950.023.83225333
CTGGGTA8700.023.5860622
TGGTGTA9300.023.51598410
AAGCATC9100.023.43941516
Produced by FastQC (version 0.11.5)