FastQCFastQC Report
Wed 25 May 2016
SRR2074738_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074738_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2412626
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC31330.12985850272690422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT7050.040.21222738
CGTCGTG7300.038.835139
CGTGGTA7500.037.7994942
TAGCGTC7500.037.7994936
AGCGTCG7850.037.14599637
GTAGCGT7750.036.9285435
AGTAGCG9350.031.18674734
GTCGTGG9650.029.9374240
GGGTAGT10000.028.3496224
TCGTGGT10100.028.33625241
GTGTAAG11350.026.40493212
GTAGTCT11100.026.02667626
GGTATTC8850.025.93185645
GGTATCA37550.025.669961
GTATTCC8900.025.48280346
GTAAGCA11850.025.2918514
TGGGTAG11400.024.868623
TAGTCTG12150.024.44411527
TAAGCAT12500.024.4091915
GGTAGTC11850.024.3794225
Produced by FastQC (version 0.11.5)