FastQCFastQC Report
Wed 25 May 2016
SRR2074737_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074737_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804690
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC31790.1133458599702641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG7550.042.5554637
GCGTCGT7850.040.9291438
TAGCGTC7800.040.8453636
GTAGCGT7850.040.58519735
CGTGGTA8150.039.42254642
CGTCGTG8600.037.0457939
AGTAGCG8950.036.50208334
TAGCGGA4100.034.24323734
TCGTGGT9700.033.6797641
GGGTAGT9300.033.38704324
ATTTACG4000.031.04939754
ATTGGCG4700.031.02177821
GGTATTC9000.029.39942745
GTCGTGG11100.028.94538140
GGTAGTC10900.028.73338325
GTAGTCT11250.027.5994626
GTGTAAG11900.026.77306412
TATGCCG2550.026.47007243
GTAAGCA12700.025.51176814
GGTAGCG5750.025.35602832
Produced by FastQC (version 0.11.5)