FastQCFastQC Report
Wed 25 May 2016
SRR2074736_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074736_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047291
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCG4500.042.0003421
TAGCGGA4950.038.72569734
ATTTACG4550.038.5698654
GGTAGCG5950.031.76341432
GTAGCGG6200.030.91809733
TTGGCGT6050.029.90107222
AGCGGAT6250.029.80678735
AAGGTAG7150.027.5653130
TCGTATG2400.025.87394740
GGTATCA35300.025.778931
TATAACG1201.3442332E-924.752622
CGGATGA7700.024.54445637
ACAACGC6700.024.1816644
GCGTGAA7400.024.08010325
GCCATAA7900.023.58131848
GTATCAA60300.023.420391
AGGTAGC7850.023.38758331
GCGGATG8100.023.33238436
AGCCATA8050.022.80652447
GGCGCCA8000.022.61323515
Produced by FastQC (version 0.11.5)