FastQCFastQC Report
Wed 25 May 2016
SRR2074735_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074735_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2689455
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGA3500.031.62800634
CGTCGTG4350.031.03392639
GTAGCGT4300.030.76689135
GCGTGAA3600.030.00002125
AGCGTCG4650.029.03173837
CGTGGTA4750.028.42054442
GGTATCA39900.028.2196391
ATTTACG3350.028.2084554
TAGCGTC5050.026.73219536
ATTGGCG3850.026.6493721
GGTAGCG4100.026.34099232
GCGTCGT5250.025.71382338
AGTAGCG5500.024.54501334
GGCGTGA4800.023.62501524
TCGTGGT6000.022.49959641
GTATCAA67350.022.4110661
GGTATTC5250.022.1138945
CGCCATT4850.021.71175817
AGCGGAT5300.021.39584435
GTAGCGG5450.020.80696733
Produced by FastQC (version 0.11.5)