FastQCFastQC Report
Wed 25 May 2016
SRR2074734_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074734_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923915
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC23390.12157501760732674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCG3800.041.2103921
GCGTCGT5500.039.7624738
GTAGCGT5850.039.6911435
TAGCGTC5800.039.56780236
CGTCGTG5550.038.91777839
CGTGGTA5800.038.63679542
TAGCGGA4400.037.43071734
AGCGTCG6000.037.34890437
AGTAGCG6300.037.28462234
ATTTACG4000.035.10170454
TATGCCG2200.033.1353943
GGTAGCG5100.032.2931732
AGCGGAT5150.031.97964335
TCGTGGT7100.031.56245441
GGTATTC5500.030.92877845
TCGTATG2200.030.68091640
ATGCGAC1500.030.59989714
GTCGTGG7350.030.48890140
GTAGCGG5500.029.94457633
GGGTAGT7350.029.75422924
Produced by FastQC (version 0.11.5)