FastQCFastQC Report
Wed 25 May 2016
SRR2074733_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074733_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101165
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC41060.19541540050400613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG8750.044.4331939
GTAGCGT9150.042.78582835
TAGCGTC9400.041.64790736
AGTAGCG9800.041.32551234
AGCGTCG9800.040.49937
GCGTCGT9800.039.39698838
CGTGGTA10000.039.14903342
TCGTGGT10100.038.7614241
GTCGTGG11050.036.8950240
GGGTAGT12250.033.9420224
GGTATTC9250.033.56753545
GTGTAAG12050.032.9377312
GGTGTAA12150.032.88886311
GTATTCC9350.032.6309946
TGGTGTA12200.032.09013710
GAGTAGC12550.031.62471633
TGGTATT12200.031.42620344
GTAAGCA13000.031.1545614
TAAGCAT13200.030.88707215
CTGGGTA13900.030.30213422
Produced by FastQC (version 0.11.5)