FastQCFastQC Report
Wed 25 May 2016
SRR2074732_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074732_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1696536
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCCATAATTTACG26600.15679007106244724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGA6000.044.09915534
ATTGGCG5850.043.3837921
AGCGGAT6200.041.80565635
TCGTATG2200.041.72647540
ATTTACG5750.040.8513954
GGTAGCG6900.039.12968432
TATGCCG2450.035.26463343
GTAGCGG7800.034.96087333
CGGATGA7300.034.7664637
CGTATGC2600.034.26857841
GCGGATG8100.032.33271836
GAGGCGC8350.032.01419413
TTGGCGT8300.030.5777322
CTCGTAT3050.030.09778639
AGCCATA7800.029.76866347
GGCGCCA9000.029.40117315
AAGGTAG8950.028.9603430
GCCGTCT3200.028.6869546
AGGTAGC9350.028.58768831
TATTGAG9500.028.4213479
Produced by FastQC (version 0.11.5)