FastQCFastQC Report
Wed 25 May 2016
SRR2074731_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074731_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651871
Sequences flagged as poor quality0
Sequence length60
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCG2950.039.35520621
TAGCGGA3450.035.2167434
ATTTACG3200.034.5931154
TCGTATG1650.032.7266740
AGCGGAT3850.032.25914435
GGTAGCG3900.031.1532732
TATGCCG1500.030.59943443
GTCCGTT901.7043931E-929.99944349
TTGGCGT3950.028.02479722
GTAGCGG4400.027.61312533
CGTATGC2000.025.64952741
AGGTAGC5450.025.26558731
GCGGATG5350.024.72851436
CTCGTAT2300.024.65171839
AAGGTAG5600.024.5888330
ACAACGC4550.024.3321674
TACGTCG801.8545526E-523.62456321
CGGATGA5250.023.1424337
TCTGTCG4800.022.4995848
AACAACG5050.021.9230423
Produced by FastQC (version 0.11.5)