FastQCFastQC Report
Wed 25 May 2016
SRR2074730_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2074730_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866159
Sequences flagged as poor quality0
Sequence length60
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGC44790.24001170318284776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT9600.046.40507535
AGTAGCG10000.045.35885234
GCGTCGT9850.045.2272838
TAGCGTC9800.045.1825336
CGTCGTG9750.045.13731839
AGCGTCG10250.043.98913237
CGTGGTA9800.043.5295142
GGTATTC9400.040.49897445
TCGTGGT10900.039.87972641
GTCGTGG11600.038.17144840
GTATTCC10050.037.6109946
GGGTAGT12050.036.7469424
GTGTAAG11800.036.15453312
TAAGCAT12250.035.9285115
CTGGGTA12350.035.41800322
TGGTATT12000.034.8741244
GTAAGCA13000.034.06341614
TGGTGTA12450.034.04915610
CTGAGTA13350.033.9766731
GGTGTAA12950.033.77701611
Produced by FastQC (version 0.11.5)