##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049546_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	66642938
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36195060908029	31.0	31.0	34.0	29.0	34.0
2	31.55366925749882	33.0	31.0	34.0	30.0	34.0
3	31.693448389085127	33.0	31.0	34.0	30.0	34.0
4	35.2401865746075	37.0	35.0	37.0	33.0	37.0
5	35.206628900424526	37.0	35.0	37.0	33.0	37.0
6	35.198348968348306	37.0	35.0	37.0	32.0	37.0
7	35.214042964312284	37.0	35.0	37.0	32.0	37.0
8	35.23435752487383	37.0	35.0	37.0	33.0	37.0
9	36.847549428268	39.0	37.0	39.0	33.0	39.0
10-11	36.82682782532787	39.0	37.0	39.0	33.0	39.0
12-13	36.800746869833375	39.0	37.0	39.0	33.0	39.0
14-15	38.06328572278731	40.0	38.0	41.0	33.0	41.0
16-17	38.01679790137703	40.0	38.0	41.0	33.0	41.0
18-19	38.033905070331684	40.0	38.0	41.0	33.0	41.0
20-21	38.01286509157204	40.0	38.0	41.0	33.0	41.0
22-23	37.93836363426834	40.0	38.0	41.0	33.0	41.0
24-25	37.92237109954546	40.0	38.0	41.0	33.0	41.0
26-27	37.84758643143854	40.0	38.0	41.0	33.0	41.0
28-29	37.76962255925751	40.0	38.0	41.0	33.0	41.0
30-31	37.70684635782414	40.0	38.0	41.0	32.5	41.0
32-33	37.62686872388489	40.0	38.0	41.0	32.0	41.0
34-35	37.52279354790751	40.0	37.5	41.0	32.0	41.0
36-37	37.45712547967198	40.0	37.0	41.0	32.0	41.0
38-39	37.35779133867117	40.0	37.0	41.0	32.0	41.0
40-41	37.254872084120905	40.0	37.0	41.0	31.5	41.0
42-43	37.1085259326352	40.0	37.0	41.0	31.0	41.0
44-45	36.96121222926877	40.0	36.0	41.0	31.0	41.0
46-47	36.8254535686887	40.0	36.0	41.0	31.0	41.0
48-49	36.6976737805287	39.5	36.0	41.0	30.5	41.0
50-51	36.53503490497373	39.0	35.0	41.0	30.0	41.0
52-53	36.35365982814263	39.0	35.0	41.0	30.0	41.0
54-55	36.164973489013946	39.0	35.0	41.0	30.0	41.0
56-57	35.94756470670606	39.0	35.0	41.0	29.5	41.0
58-59	35.717723031658664	38.0	35.0	40.0	29.0	41.0
60-61	35.47770670014579	38.0	35.0	40.0	29.0	41.0
62-63	35.21740073194252	37.0	34.0	40.0	28.0	41.0
64-65	34.9591909423321	37.0	34.0	40.0	28.0	41.0
66-67	34.67829210951054	36.5	34.0	39.5	28.0	41.0
68-69	34.36939522384202	36.0	34.0	39.0	28.0	41.0
70-71	34.0746568901269	35.0	34.0	39.0	28.0	41.0
72-73	33.75084116939742	35.0	34.0	38.5	27.0	40.0
74-75	33.4397654182053	35.0	33.0	37.0	27.0	39.5
76-77	33.14179644961031	35.0	33.0	37.0	27.0	39.0
78-79	32.838304352668246	35.0	33.0	36.5	26.0	39.0
80-81	32.56796618870555	35.0	33.0	36.0	26.0	38.0
82-83	32.30595156233959	35.0	33.0	36.0	26.0	37.0
84-85	32.06134232857501	35.0	33.0	35.0	26.0	37.0
86-87	31.874875992111875	35.0	33.0	35.0	25.0	36.5
88-89	31.700110010155914	35.0	33.0	35.0	25.0	36.0
90-91	31.55131643505873	35.0	33.0	35.0	25.0	36.0
92-93	31.388482002399115	35.0	33.0	35.0	24.5	35.5
94-95	31.21809801512652	35.0	32.5	35.0	24.0	35.0
96-97	31.063709338564877	35.0	32.0	35.0	24.0	35.0
98-99	30.92621141642945	35.0	32.0	35.0	23.0	35.0
100	30.809312338540657	35.0	32.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5808820963731272
1101	2	0.5253553097710864
1101	3	0.5489418527887793
1101	4	0.4699289737870771
1101	5	0.45633874927492
1101	6	0.5146780712702039
1101	7	0.5064753948417717
1101	8	0.5088236787556326
1101	9	0.5855207182330489
1101	10-11	0.5759752903011659
1101	12-13	0.6035256925520258
1101	14-15	0.7277547559443533
1101	16-17	0.7652019525763691
1101	18-19	0.7380151852742145
1101	20-21	0.7531287121681203
1101	22-23	0.7657351808102035
1101	24-25	0.7525761748069968
1101	26-27	0.7671802057219352
1101	28-29	0.7750726326539166
1101	30-31	0.7649569452959639
1101	32-33	0.7732285825401561
1101	34-35	0.8108741767338117
1101	36-37	0.7852850797562567
1101	38-39	0.797114767589143
1101	40-41	0.804266030127252
1101	42-43	0.8055460572998925
1101	44-45	0.8088560828165541
1101	46-47	0.7755304580467879
1101	48-49	0.7879710051754714
1101	50-51	0.7975175910934524
1101	52-53	0.7677911225512233
1101	54-55	0.754945975969072
1101	56-57	0.7834738983897154
1101	58-59	0.7652214087940621
1101	60-61	0.797270411343753
1101	62-63	0.7549341425267428
1101	64-65	0.804579818060553
1101	66-67	0.7793357832588867
1101	68-69	0.7650886942898865
1101	70-71	0.7563392733679848
1101	72-73	0.7722661756947176
1101	74-75	0.747495738662387
1101	76-77	0.7366672145368298
1101	78-79	0.7381378586058531
1101	80-81	0.7147489687765329
1101	82-83	0.7731793894672236
1101	84-85	0.7422996599582845
1101	86-87	0.7022236814733525
1101	88-89	0.7223823029235881
1101	90-91	0.73181214197038
1101	92-93	0.7347416637566013
1101	94-95	0.7551936356001896
1101	96-97	0.728103069492299
1101	98-99	0.753328119774352
1101	100	0.7265681904327188
1102	1	0.4869451597002481
1102	2	0.4389716193667397
1102	3	0.4674197918554128
1102	4	0.42621987545643947
1102	5	0.3745854182260473
1102	6	0.40917032268483666
1102	7	0.42097065370417397
1102	8	0.4207474829341109
1102	9	0.5014745549862454
1102	10-11	0.5306050840015573
1102	12-13	0.5229447056744121
1102	14-15	0.6464338649467578
1102	16-17	0.651416882974182
1102	18-19	0.6015298150139827
1102	20-21	0.667046623889
1102	22-23	0.6820767759401534
1102	24-25	0.6380134331770648
1102	26-27	0.6397032917871641
1102	28-29	0.6681246893761283
1102	30-31	0.6614807076102238
1102	32-33	0.668446630567999
1102	34-35	0.679170309081158
1102	36-37	0.6815792445599058
1102	38-39	0.6858323167454756
1102	40-41	0.6590715216193175
1102	42-43	0.6304906824962799
1102	44-45	0.6496733884141719
1102	46-47	0.6702106677126523
1102	48-49	0.6630541681074433
1102	50-51	0.6988351494507867
1102	52-53	0.6965953946559225
1102	54-55	0.6632219092473406
1102	56-57	0.6965699367100413
1102	58-59	0.6960963636498718
1102	60-61	0.6932155952130614
1102	62-63	0.6458012405959863
1102	64-65	0.6483308498822353
1102	66-67	0.6661899876921282
1102	68-69	0.6403847523599495
1102	70-71	0.6549142317840193
1102	72-73	0.6629825562921354
1102	74-75	0.6048302237454521
1102	76-77	0.6128898086403112
1102	78-79	0.6097696850007637
1102	80-81	0.6192370597351271
1102	82-83	0.62184923573475
1102	84-85	0.6499034661815415
1102	86-87	0.631767844018345
1102	88-89	0.6585153647180668
1102	90-91	0.6265782396212032
1102	92-93	0.6458124745603797
1102	94-95	0.6613094618115838
1102	96-97	0.6361307166362273
1102	98-99	0.6246514218589923
1102	100	0.638586961896948
1103	1	0.41664076119828053
1103	2	0.3596339625546854
1103	3	0.3790533680630759
1103	4	0.34940490316655826
1103	5	0.3287142815759907
1103	6	0.3640575835147217
1103	7	0.363970984130205
1103	8	0.33311652805042513
1103	9	0.41621508653416583
1103	10-11	0.4255312110002265
1103	12-13	0.45274417078771734
1103	14-15	0.5418007744332698
1103	16-17	0.5732527311744917
1103	18-19	0.5394909849913176
1103	20-21	0.5396843985287987
1103	22-23	0.5637016827691355
1103	24-25	0.5536107568839057
1103	26-27	0.5324023449173225
1103	28-29	0.5781744956418322
1103	30-31	0.5480498653835468
1103	32-33	0.5539205694267366
1103	34-35	0.5890753256927823
1103	36-37	0.5598494814106161
1103	38-39	0.5775927456231997
1103	40-41	0.5784390514396023
1103	42-43	0.5576644282530694
1103	44-45	0.5673798071563994
1103	46-47	0.5575496505106372
1103	48-49	0.5629856527869279
1103	50-51	0.5419526909239067
1103	52-53	0.5455797371712308
1103	54-55	0.5359055426433486
1103	56-57	0.5455600862774617
1103	58-59	0.5933328899553914
1103	60-61	0.5928036330513464
1103	62-63	0.5681508475735484
1103	64-65	0.6034882087655475
1103	66-67	0.5842079588015068
1103	68-69	0.5938454791384586
1103	70-71	0.5646517523535692
1103	72-73	0.5459049286622246
1103	74-75	0.5336107367805951
1103	76-77	0.5375829578454656
1103	78-79	0.5543317322492953
1103	80-81	0.5187800586611644
1103	82-83	0.5709531965972943
1103	84-85	0.5544674440117561
1103	86-87	0.5526073233967388
1103	88-89	0.5545327092218919
1103	90-91	0.547652805185983
1103	92-93	0.5613527248402335
1103	94-95	0.5777588452972786
1103	96-97	0.567404969171708
1103	98-99	0.5397675311797983
1103	100	0.4834310989402475
1104	1	0.28223621646037245
1104	2	0.2707019356835012
1104	3	0.27600168795894575
1104	4	0.23119139378751186
1104	5	0.2487770563043057
1104	6	0.28987600439541694
1104	7	0.2922926463546105
1104	8	0.2944161728046808
1104	9	0.3689642723739297
1104	10-11	0.35092294045413297
1104	12-13	0.39129270408137273
1104	14-15	0.4639942481836812
1104	16-17	0.4838611491535971
1104	18-19	0.4634469417355831
1104	20-21	0.4886696116853315
1104	22-23	0.5170344293770128
1104	24-25	0.4902838257948332
1104	26-27	0.48118935767441684
1104	28-29	0.4735796736931164
1104	30-31	0.4693699063782333
1104	32-33	0.44555606322348495
1104	34-35	0.4735378123798384
1104	36-37	0.4874259822145177
1104	38-39	0.45659269288685067
1104	40-41	0.4327966497098501
1104	42-43	0.45010271596247975
1104	44-45	0.4477232248878664
1104	46-47	0.449008465257009
1104	48-49	0.42905313891776586
1104	50-51	0.46150641968784356
1104	52-53	0.46629830105843695
1104	54-55	0.4543848958714136
1104	56-57	0.4817474016156851
1104	58-59	0.4993137654395383
1104	60-61	0.47020114974936433
1104	62-63	0.4387912917882062
1104	64-65	0.4382866111668804
1104	66-67	0.4553117413833476
1104	68-69	0.48446838361211064
1104	70-71	0.49248743372577763
1104	72-73	0.4766082776147087
1104	74-75	0.4639009420895661
1104	76-77	0.47546417717231293
1104	78-79	0.46757662102832853
1104	80-81	0.4248130872763056
1104	82-83	0.4513009927461624
1104	84-85	0.4629288134022005
1104	86-87	0.4408840218665553
1104	88-89	0.45465360693405543
1104	90-91	0.4501545556419515
1104	92-93	0.4620436399982921
1104	94-95	0.4563886829282069
1104	96-97	0.4368934127456363
1104	98-99	0.4205946724648335
1104	100	0.43989991189948796
1105	1	0.2819675979793175
1105	2	0.2710975334221466
1105	3	0.2910092597759828
1105	4	0.2940706560524049
1105	5	0.25159882709283465
1105	6	0.29303004414613554
1105	7	0.2989358906084405
1105	8	0.28018719352718335
1105	9	0.32007928252189544
1105	10-11	0.3541738487106869
1105	12-13	0.3601510409334736
1105	14-15	0.40399253841739124
1105	16-17	0.4572087088414918
1105	18-19	0.43059822236413936
1105	20-21	0.42066583690296255
1105	22-23	0.4688531378255121
1105	24-25	0.4494444325758167
1105	26-27	0.4452411559474925
1105	28-29	0.4566155316353928
1105	30-31	0.44373081923711766
1105	32-33	0.4358761790251293
1105	34-35	0.480679736751938
1105	36-37	0.4671226416632024
1105	38-39	0.4553646739775772
1105	40-41	0.47760996543261314
1105	42-43	0.45020666622786365
1105	44-45	0.4160134635312005
1105	46-47	0.4041302326209504
1105	48-49	0.3920447800211022
1105	50-51	0.40542112524904184
1105	52-53	0.4319856360788279
1105	54-55	0.4723377529124164
1105	56-57	0.44463825192517703
1105	58-59	0.4456649025318882
1105	60-61	0.43882437558971077
1105	62-63	0.4041253121769941
1105	64-65	0.41766168466833165
1105	66-67	0.45727460144343723
1105	68-69	0.45147235710044953
1105	70-71	0.44065529002947557
1105	72-73	0.42980370713711125
1105	74-75	0.412804173403579
1105	76-77	0.4091586932810287
1105	78-79	0.41123907830662176
1105	80-81	0.3905002813828702
1105	82-83	0.40440570154816413
1105	84-85	0.4173058639984788
1105	86-87	0.42039073486292367
1105	88-89	0.37979864278544895
1105	90-91	0.4044533298435198
1105	92-93	0.4444335631047096
1105	94-95	0.43891499067359874
1105	96-97	0.43855399742846757
1105	98-99	0.4206674780877151
1105	100	0.4129373361761637
1106	1	0.2300722741714587
1106	2	0.2251250453262763
1106	3	0.20596628007683648
1106	4	0.18865393644667705
1106	5	0.16740625359022943
1106	6	0.2336028994727144
1106	7	0.2436730350312999
1106	8	0.22787004484016649
1106	9	0.27489438893564966
1106	10-11	0.29092138770128173
1106	12-13	0.3150462332455177
1106	14-15	0.37723325757503545
1106	16-17	0.41467055860243107
1106	18-19	0.38535839434674557
1106	20-21	0.374731379272049
1106	22-23	0.3567499813229418
1106	24-25	0.3648704526975166
1106	26-27	0.37596128030074993
1106	28-29	0.3880339912716124
1106	30-31	0.36557518002749134
1106	32-33	0.3662536250559043
1106	34-35	0.3814025873281395
1106	36-37	0.3323088857519778
1106	38-39	0.36795593956416894
1106	40-41	0.3685693197007254
1106	42-43	0.3684327355474579
1106	44-45	0.3811705057006236
1106	46-47	0.3774525398941364
1106	48-49	0.37637811604268023
1106	50-51	0.3652765358664709
1106	52-53	0.3785424595086013
1106	54-55	0.4054373418843227
1106	56-57	0.38388127291211305
1106	58-59	0.40212318332408614
1106	60-61	0.4024532059028516
1106	62-63	0.3851610955566187
1106	64-65	0.38709787924507566
1106	66-67	0.40028379231363687
1106	68-69	0.3748294656828506
1106	70-71	0.39109363927698837
1106	72-73	0.38354099379932904
1106	74-75	0.3803467649974053
1106	76-77	0.39176642667812445
1106	78-79	0.3614207025292586
1106	80-81	0.4055501950834781
1106	82-83	0.39924112774664167
1106	84-85	0.39239391364316134
1106	86-87	0.38370679294665777
1106	88-89	0.3946371329751379
1106	90-91	0.35713034210688477
1106	92-93	0.4262492951950634
1106	94-95	0.3956048052318373
1106	96-97	0.3595803773270525
1106	98-99	0.36641648906845603
1106	100	0.3524403641991114
1107	1	0.2153075097509216
1107	2	0.20853340877378912
1107	3	0.1971962835487382
1107	4	0.18708600244398355
1107	5	0.1581375795626485
1107	6	0.16183657439477628
1107	7	0.19745536817226395
1107	8	0.16279267136139453
1107	9	0.23314079086805606
1107	10-11	0.22212537574090874
1107	12-13	0.24262793853172582
1107	14-15	0.2870611991573213
1107	16-17	0.30221968766126395
1107	18-19	0.29239194760958753
1107	20-21	0.2546051617713445
1107	22-23	0.28795106466773035
1107	24-25	0.244004661351525
1107	26-27	0.2827066768011761
1107	28-29	0.3148781808798873
1107	30-31	0.25758883006332667
1107	32-33	0.28260381560431824
1107	34-35	0.2401674810411265
1107	36-37	0.26003621068603877
1107	38-39	0.25824752247167027
1107	40-41	0.28925732245233604
1107	42-43	0.29038857441499744
1107	44-45	0.303742746512242
1107	46-47	0.28652611360460867
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2216	100	-1.1136959484044056
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	565343.0
3	122415.0
4	94082.0
5	82640.0
6	83374.0
7	85759.0
8	90074.0
9	103099.0
10	113498.0
11	123238.0
12	130977.0
13	135634.0
14	140801.0
15	144087.0
16	147357.0
17	152172.0
18	156087.0
19	161125.0
20	168203.0
21	178887.0
22	199500.0
23	232065.0
24	284847.0
25	358269.0
26	453762.0
27	573950.0
28	722615.0
29	895307.0
30	1120184.0
31	1428131.0
32	1844440.0
33	2451144.0
34	3432451.0
35	5211050.0
36	8809282.0
37	1.4446598E7
38	1.6507796E7
39	4683885.0
40	8810.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.618771466302892	27.804235157067808	25.025932496843424	23.551060879785883
2	24.288153536632493	27.511174424779206	24.89805161166055	23.302620426927753
3	24.962633587120713	25.384539937605645	24.87254782587217	24.78027864940147
4	24.987027103794095	25.38357905354311	25.076585856652933	24.55280798600986
5	24.930447370295113	25.96200808286313	25.254704610246385	23.85283993659537
6	24.915071691396903	25.63783718818034	24.544090694156207	24.90300042626655
7	24.611548470627874	25.611030388594862	25.474316257028573	24.303104883748688
8	24.260465391474995	25.823962640016035	25.521022461957525	24.394549506551442
9	24.16572187846882	25.560209588699678	25.6214186806545	24.652649852177003
10-11	24.203078445570195	25.818869294554965	25.66145749163329	24.316594768241558
12-13	24.107195418090264	25.5436943269258	25.909049084533237	24.440061170450697
14-15	24.069544435994782	25.45259417050005	25.983593396337934	24.49426799716723
16-17	24.086723055323503	25.355404761918702	25.992891341616907	24.564980841140883
18-19	24.1308020656427	25.20045535569102	25.9148335788283	24.753908999837982
20-21	24.170629353912567	25.156801328134833	25.891597310161185	24.78097200779142
22-23	24.25651249860927	25.192758841261504	25.80550393286019	24.745224727269036
24-25	24.307391575662276	25.138200602445536	25.710381342594285	24.844026479297902
26-27	24.354701320136996	25.13954554427798	25.67734144512616	24.828411690458868
28-29	24.384649686851347	25.20117361484255	25.635972289248244	24.778204409057857
30-31	24.45118910750447	25.11235060366392	25.584283391733887	24.85217689709772
32-33	24.489791291238525	25.116043177699606	25.547320618468618	24.846844912593262
34-35	24.514988102576787	25.159093048298274	25.51615578725529	24.809763061869642
36-37	24.527448144437145	25.076197620968983	25.490803030050902	24.905551204542974
38-39	24.54519513049726	25.05414113039303	25.492688942392387	24.90797479671732
40-41	24.564837423801716	25.105651159559635	25.462725576491707	24.866785840146942
42-43	24.61521469810742	25.03245108937732	25.40004149468172	24.952292717833544
44-45	24.62421979288642	25.015500447975132	25.416495285384766	24.94378447375368
46-47	24.647610436650705	25.055629270955947	25.37583150628674	24.920928786106604
48-49	24.66195216085346	24.993430173962057	25.332890271338826	25.011727393845657
50-51	24.667592301545767	25.005829961802878	25.347602320354085	24.97897541629727
52-53	24.680706788404365	25.036553203064184	25.32105235526207	24.961687653269383
54-55	24.721082778309505	24.98571817381074	25.27939825815532	25.01380078972444
56-57	24.717775037312723	24.984333383819887	25.278269677678107	25.019621901189282
58-59	24.72204662395665	25.03306344828226	25.26780548264404	24.977084445117047
60-61	24.751200103864203	24.99353589506171	25.200897863687842	25.054366137386243
62-63	24.755792882171875	24.97666952068938	25.225940711657863	25.04159688548088
64-65	24.768226179826115	25.043846381229727	25.20019973936851	24.98772769957565
66-67	24.801591449046903	24.98961205778088	25.154464143480865	25.054332349691354
68-69	24.787074732635954	24.990624207575056	25.165219427038444	25.05708163275054
70-71	24.830304661037196	25.03272001754399	25.13737706583361	24.9995982555852
72-73	24.85344312716821	25.01864092341351	25.095173617639194	25.03274233177909
74-75	24.853815684471403	25.02415327220721	25.10584480006772	25.016186243253667
76-77	24.864053887624337	25.07463108456794	25.090034281669677	24.971280746138046
78-79	24.908881008469557	25.030953827789276	25.052451820832992	25.00771334290818
80-81	24.894585766997913	25.053946052648246	25.067155614442605	24.984312565911235
82-83	24.920438766929912	25.096383261610715	25.05798451625361	24.92519345520576
84-85	24.94232494686078	25.057006583286395	25.005406719365826	24.995261750487003
86-87	24.930325784679766	25.078584389805336	25.00443143290887	24.986658392606024
88-89	24.954811727916283	25.116466130212974	24.985347849996675	24.943374291874072
90-91	24.974188717069335	25.089092689003767	24.948406346560446	24.98831224736645
92-93	24.96672172484581	25.10358824594256	24.961764235318505	24.967925793893126
94-95	24.98926989316301	25.142334427895847	24.940216581600776	24.92817909734037
96-97	25.013018898623102	25.12694740211775	24.893446719845766	24.96658697941338
98-99	25.014633409447462	25.152083262445764	24.88881744081173	24.94446588729504
100	25.0421331340789	25.192245451297246	24.85210082809115	24.913520586532705
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1351.0
1	2715.0
2	5634.5
3	7515.0
4	6929.0
5	5252.0
6	4054.5
7	3382.0
8	2933.5
9	2549.5
10	2189.5
11	1940.5
12	1867.0
13	1897.5
14	2063.5
15	2515.0
16	3407.0
17	5020.0
18	8053.5
19	13458.5
20	22203.0
21	35351.5
22	54302.0
23	81176.0
24	116410.5
25	160014.5
26	213001.0
27	275670.5
28	348356.5
29	431221.0
30	521698.0
31	620778.5
32	730363.0
33	850079.0
34	979547.0
35	1118652.0
36	1271526.0
37	1442658.5
38	1629846.0
39	1814979.0
40	1978966.5
41	2113607.0
42	2204837.5
43	2247791.5
44	2249859.5
45	2211436.5
46	2136890.5
47	2046962.5
48	1957542.5
49	1876496.0
50	1807999.5
51	1754838.5
52	1718252.0
53	1704398.5
54	1717483.0
55	1752372.5
56	1797716.0
57	1826487.5
58	1824121.5
59	1794963.0
60	1744850.0
61	1670865.0
62	1576378.0
63	1464403.0
64	1335188.0
65	1189482.5
66	1038191.0
67	898439.0
68	767906.5
69	644992.5
70	536940.5
71	442644.5
72	359847.0
73	289914.5
74	232187.0
75	186889.5
76	151344.0
77	123138.5
78	100267.5
79	80886.0
80	64961.0
81	52230.0
82	41637.5
83	32666.0
84	25099.5
85	18837.5
86	13914.5
87	10034.0
88	7141.0
89	4950.5
90	3420.0
91	2412.5
92	1626.5
93	1120.5
94	769.5
95	545.5
96	395.0
97	288.0
98	219.0
99	190.0
100	457.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08493022921648503
2	0.03444325938931444
3	0.006326251702768565
4	0.006005137408557828
5	0.10391348592704601
6	0.15719445022066703
7	0.07388179674791648
8	0.07788222061878484
9	0.06505565525937647
10-11	0.05037968164008615
12-13	0.0421567548537551
14-15	0.056089213833879895
16-17	0.0789205902056719
18-19	0.0647232869595275
20-21	0.06915211331169104
22-23	0.06852714086524817
24-25	0.08354073465368528
26-27	0.09946590289881876
28-29	0.08251962120877684
30-31	0.07261309517896705
32-33	0.06556958818352215
34-35	0.08409068039587331
36-37	0.08730407413910833
38-39	0.08935380369935071
40-41	0.08189539902937652
42-43	0.0877834947792968
44-45	0.09756997808229884
46-47	0.10177372432169782
48-49	0.08450932940561534
50-51	0.0902856353661959
52-53	0.09340449546206982
54-55	0.1019515376107818
56-57	0.10122227804542472
58-59	0.09395894280651312
60-61	0.08401940502683121
62-63	0.08436302733231839
64-65	0.09930234468354321
66-67	0.11129686389276536
68-69	0.10088390760923537
70-71	0.08750514570651131
72-73	0.09143654500946521
74-75	0.11229321852526969
76-77	0.09394018613044941
78-79	0.10744949449857687
80-81	0.08792304444921081
82-83	0.09985154015868868
84-85	0.10953148554164883
86-87	0.08844823137899473
88-89	0.0819104043702275
90-91	0.0938719118295775
92-93	0.1155231181434408
94-95	0.08891864881467261
96-97	0.08271544090688199
98-99	0.09237963068194863
100	0.1122144404858021
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6.6642938E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.66571598878646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.31630365797848	60.04175493534135
2	15.839675180256862	24.28720077448405
3	4.123158567328698	9.48314711098661
4	1.1687571312575453	3.5841440913943914
5	0.34180266757946426	1.3102273118428396
6	0.11210050968294176	0.515655950253037
7	0.04134430773346438	0.22187836681127376
8	0.016054000424191986	0.0984633149603968
9	0.010054937410299591	0.06937820782047473
>10	0.030580534606989392	0.37975501163715925
>50	1.6850574103743267E-4	0.008394924468408144
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1254005638226815E-4	6.002136340387634E-6	3.001068170193817E-6	4.501602255290726E-6	0.0
2	1.2004272680775268E-4	7.502670425484543E-6	6.002136340387634E-6	1.050373859567836E-5	1.5005340850969086E-6
3	1.2604486314814033E-4	1.5005340850969085E-5	7.502670425484543E-6	1.5005340850969085E-5	9.003204510581452E-6
4	1.3204699948852794E-4	1.6505874936065993E-5	9.003204510581452E-6	2.7009613531744355E-5	1.5005340850969085E-5
5	1.395496699140125E-4	2.5509079446647444E-5	1.050373859567836E-5	3.4512283957228896E-5	1.8006409021162904E-5
6	1.4405127216930323E-4	2.8510147616841263E-5	1.050373859567836E-5	4.051442029761653E-5	2.250801127645363E-5
7	1.4855287442459394E-4	3.3011749872131985E-5	1.8006409021162904E-5	4.501602255290726E-5	2.250801127645363E-5
8	1.605571471053692E-4	3.4512283957228896E-5	1.950694310625981E-5	5.25186929783918E-5	2.5509079446647444E-5
9	1.6956035161595065E-4	4.051442029761653E-5	1.950694310625981E-5	5.7020295233682526E-5	3.001068170193817E-5
10-11	1.815646242967259E-4	4.8017090723101074E-5	2.0257210148808267E-5	6.752403382936087E-5	3.4512283957228896E-5
12-13	1.9131809584985583E-4	6.002136340387634E-5	2.250801127645363E-5	8.177910763778152E-5	4.1264687340164985E-5
14-15	2.0257210148808265E-4	7.127536904210315E-5	2.62593464891959E-5	9.52839144036537E-5	4.576628959545571E-5
16-17	2.2883144797727854E-4	9.003204510581452E-5	3.4512283957228896E-5	1.0878872116952587E-4	5.026789185074644E-5
18-19	2.4833839108353835E-4	9.753471553129907E-5	4.201495438271344E-5	1.2079299385030113E-4	5.101815889329489E-5
20-21	2.715966694025405E-4	1.0578765299933205E-4	5.026789185074644E-5	1.2979619836088257E-4	5.101815889329489E-5
22-23	3.001068170193817E-4	1.1028925525462279E-4	5.777056227623098E-5	1.4555180625440013E-4	5.25186929783918E-5
24-25	3.2561589646602914E-4	1.2904593131833413E-4	6.152189748897326E-5	1.575560789351754E-4	5.25186929783918E-5
26-27	3.5637684521051577E-4	1.4180047104165786E-4	6.827430087190934E-5	1.7181115274359602E-4	5.476949410603716E-5
28-29	3.796351235295179E-4	1.4705234033949704E-4	7.502670425484542E-5	1.7931382316908057E-4	5.927109636132789E-5
30-31	4.1564794157184366E-4	1.5230420963733621E-4	8.02785735526846E-5	1.9281862993495276E-4	6.37726986166186E-5
32-33	4.614142311672994E-4	1.6130741414791767E-4	8.402990876542687E-5	2.0932450487101873E-4	7.277590312720007E-5
34-35	5.034291855500128E-4	1.7106088570104756E-4	8.402990876542687E-5	2.2432984572198783E-4	8.02785735526846E-5
36-37	5.446938728901778E-4	1.7931382316908057E-4	8.553044285052379E-5	2.363341184027631E-4	8.252937468032997E-5
38-39	6.024644351664088E-4	1.8981756176475893E-4	8.928177806326605E-5	2.528399933388291E-4	8.853151102071761E-5
40-41	6.564836622298975E-4	2.085742378284703E-4	9.378338031855679E-5	2.700961353174435E-4	9.228284623345987E-5
42-43	7.127536904210315E-4	2.3183251614747238E-4	9.82849825738475E-5	2.8210040799821884E-4	9.453364736110524E-5
44-45	8.057868036970399E-4	2.4683785699844147E-4	1.0578765299933205E-4	3.0460841927467243E-4	9.453364736110524E-5
46-47	8.838145761220792E-4	2.62593464891959E-4	1.0953898821207433E-4	3.151121578703508E-4	9.67844484887506E-5
48-49	9.92603297291605E-4	2.8435120912586416E-4	1.117897893397197E-4	3.263661635085776E-4	9.753471553129905E-5
50-51	0.001124650296780133	3.0310788518957555E-4	1.147908575099135E-4	3.458731066148374E-4	9.903524961639597E-5
52-53	0.001289709046140793	3.2411536238093226E-4	1.1629139159501041E-4	3.571271122530642E-4	1.0128605074404134E-4
54-55	0.0015087870225649414	3.338688339340622E-4	1.1854219272265578E-4	3.68381117891291E-4	1.0428711891423515E-4
56-57	0.0017301158001167356	3.481239077424828E-4	1.2229352793539802E-4	3.818859246571632E-4	1.050373859567836E-4
58-59	0.0019799547252853707	3.608784474658065E-4	1.2604486314814033E-4	3.9914206663577763E-4	1.1103952229717123E-4
60-61	0.0022575535310282993	3.6763085084874257E-4	1.2904593131833413E-4	4.201495438271344E-4	1.1404059046736505E-4
62-63	0.002583169427494328	3.886383280400993E-4	1.3054646540343104E-4	4.3590515172065193E-4	1.1554112455246196E-4
64-65	0.003022075647385174	4.0664473706126223E-4	1.3729886878636712E-4	4.471591573588788E-4	1.1554112455246196E-4
66-67	0.003472986139956795	4.2390087903987665E-4	1.433010051267548E-4	4.644152993374932E-4	1.1629139159501041E-4
68-69	0.004019930813974618	4.396564869333942E-4	1.4405127216930323E-4	4.8392224244375303E-4	1.1779192568010731E-4
70-71	0.004682416612544904	4.531612936992664E-4	1.463020732969486E-4	5.086810548478519E-4	1.1854219272265578E-4
72-73	0.005494205552582331	4.6591583342259007E-4	1.5005340850969085E-4	5.244366627413695E-4	1.2229352793539805E-4
74-75	0.006488309383959032	4.8167144131610766E-4	1.5230420963733621E-4	5.349404013370478E-4	1.327972665310764E-4
76-77	0.00773675374275966	5.026789185074644E-4	1.605571471053692E-4	5.529468103582108E-4	1.395496699140125E-4
78-79	0.009434608060046812	5.229361286562726E-4	1.6805981753085375E-4	5.64951083038986E-4	1.4180047104165786E-4
80-81	0.011689910789947466	5.439436058476293E-4	1.740619538712414E-4	5.784558898048582E-4	1.463020732969486E-4
82-83	0.014874044118523106	5.589489466985984E-4	1.785635561265321E-4	6.032147022089571E-4	1.5230420963733621E-4
84-85	0.019383899311281864	5.777056227623098E-4	1.8081435725417747E-4	6.249724464428624E-4	1.5455501076498158E-4
86-87	0.025627621639370102	5.964622988260212E-4	1.8381542542437128E-4	6.39227520251283E-4	1.560555448500785E-4
88	0.031824827410820336	6.06215770379151E-4	1.905678288073074E-4	6.482307247618645E-4	1.590566130202723E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	11550	0.0	6.388598	94
AGATCGG	15375	0.0	6.2970915	94
>>END_MODULE