##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049546_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	66642938
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.844007267506722	33.0	31.0	34.0	30.0	34.0
2	32.166191967707064	34.0	31.0	34.0	30.0	34.0
3	32.264347949365614	34.0	31.0	34.0	30.0	34.0
4	35.78873982116455	37.0	35.0	37.0	33.0	37.0
5	35.84832502432591	37.0	35.0	37.0	35.0	37.0
6	35.87291945322099	37.0	35.0	37.0	35.0	37.0
7	35.87304567814822	37.0	35.0	37.0	35.0	37.0
8	35.8707060303974	37.0	35.0	37.0	35.0	37.0
9	37.58026460358035	39.0	37.0	39.0	35.0	39.0
10-11	37.57081426992309	39.0	37.0	39.0	35.0	39.0
12-13	37.55661332037912	39.0	37.0	39.0	35.0	39.0
14-15	38.89558531318052	40.0	38.0	41.0	35.0	41.0
16-17	38.88706278975876	40.0	38.0	41.0	35.0	41.0
18-19	38.896548641057805	40.0	38.0	41.0	35.0	41.0
20-21	38.89615718172569	40.0	38.0	41.0	35.0	41.0
22-23	38.860818118492915	40.0	38.0	41.0	35.0	41.0
24-25	38.83134316497271	40.0	38.0	41.0	35.0	41.0
26-27	38.72198981533497	40.0	38.0	41.0	34.5	41.0
28-29	38.666254194855576	40.0	38.0	41.0	34.0	41.0
30-31	38.60751690599235	40.0	38.0	41.0	34.0	41.0
32-33	38.54045475906239	40.0	38.0	41.0	34.0	41.0
34-35	38.45975746897594	40.0	38.0	41.0	34.0	41.0
36-37	38.393251750095416	40.0	38.0	41.0	34.0	41.0
38-39	38.313220487067966	40.0	38.0	41.0	34.0	41.0
40-41	38.21290592710663	40.0	38.0	41.0	33.0	41.0
42-43	38.12076658445041	40.0	37.5	41.0	33.0	41.0
44-45	38.02490191083713	40.0	37.0	41.0	33.0	41.0
46-47	37.8936994344397	40.0	37.0	41.0	33.0	41.0
48-49	37.77076482582446	40.0	37.0	41.0	33.0	41.0
50-51	37.62585875940823	40.0	36.0	41.0	33.0	41.0
52-53	37.45671310289471	39.5	36.0	41.0	32.5	41.0
54-55	37.270688441136855	39.0	35.0	41.0	32.0	41.0
56-57	37.080162462225175	39.0	35.0	41.0	32.0	41.0
58-59	36.872657820097906	39.0	35.0	41.0	31.5	41.0
60-61	36.64460156303433	38.0	35.0	40.5	31.0	41.0
62-63	36.38284446583073	38.0	35.0	40.0	31.0	41.0
64-65	36.12513203574548	37.0	35.0	40.0	31.0	41.0
66-67	35.82975995896219	37.0	35.0	40.0	31.0	41.0
68-69	35.53028170066572	36.0	34.5	39.0	30.5	41.0
70-71	35.22927498334482	36.0	34.0	39.0	30.0	41.0
72-73	34.93366821552796	35.0	34.0	39.0	30.0	40.5
74-75	34.62079207852451	35.0	34.0	37.5	30.0	40.0
76-77	34.31605270764023	35.0	34.0	37.0	30.0	39.0
78-79	34.020277407337595	35.0	34.0	36.5	30.0	39.0
80-81	33.76210291779152	35.0	34.0	36.0	29.0	38.5
82-83	33.51591152088763	35.0	34.0	36.0	29.0	37.0
84-85	33.295261239833096	35.0	34.0	35.0	29.0	37.0
86-87	33.09637760117958	35.0	33.0	35.0	29.0	36.5
88-89	32.92485021443682	35.0	33.0	35.0	29.0	36.0
90-91	32.77666317772484	35.0	33.0	35.0	29.0	36.0
92-93	32.628001837193914	35.0	33.0	35.0	29.0	36.0
94-95	32.43864806800685	35.0	33.0	35.0	29.0	35.0
96-97	32.321004049971506	35.0	33.0	35.0	29.0	35.0
98-99	32.191984355491655	35.0	33.0	35.0	29.0	35.0
100	32.08247172416078	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5708783207433861
1101	2	0.48965762382250233
1101	3	0.43772270411716363
1101	4	0.3138307930693003
1101	5	0.2604679269550587
1101	6	0.2878729921081842
1101	7	0.29058409941105623
1101	8	0.3003554645913411
1101	9	0.369606132853022
1101	10-11	0.37626496335758475
1101	12-13	0.3881113450521383
1101	14-15	0.5353679570227357
1101	16-17	0.5208626094538147
1101	18-19	0.512553215566534
1101	20-21	0.5320348448440981
1101	22-23	0.5061492035468191
1101	24-25	0.5247124180225669
1101	26-27	0.559668170080414
1101	28-29	0.5596291891693141
1101	30-31	0.5363843100212122
1101	32-33	0.534517684671421
1101	34-35	0.5445154861061496
1101	36-37	0.5592980748152598
1101	38-39	0.5890577851365677
1101	40-41	0.5830338050608148
1101	42-43	0.592170593644255
1101	44-45	0.5784156216961733
1101	46-47	0.5971404853045428
1101	48-49	0.5871472656127921
1101	50-51	0.5767005037766353
1101	52-53	0.5969621148868427
1101	54-55	0.5738535121545354
1101	56-57	0.5727185991145589
1101	58-59	0.553353259298845
1101	60-61	0.5520858038099448
1101	62-63	0.5693209746483703
1101	64-65	0.5613976313191174
1101	66-67	0.5533657095277391
1101	68-69	0.5499555402267973
1101	70-71	0.5355413410224443
1101	72-73	0.5083831066005047
1101	74-75	0.4844399947396951
1101	76-77	0.49024236225170625
1101	78-79	0.4643346701952993
1101	80-81	0.44835285908462197
1101	82-83	0.47989601446820274
1101	84-85	0.46508749791743753
1101	86-87	0.4810406450960514
1101	88-89	0.4713488132869088
1101	90-91	0.4849244014525169
1101	92-93	0.5107423190604337
1101	94-95	0.461813335721196
1101	96-97	0.5273338405022017
1101	98-99	0.5424441512932958
1101	100	0.5353898630154816
1102	1	0.5292483889378694
1102	2	0.4290178425232085
1102	3	0.3965142408587923
1102	4	0.28820348738605617
1102	5	0.2613097448141275
1102	6	0.2709586589970243
1102	7	0.27726932185852604
1102	8	0.2799401414913092
1102	9	0.342905448238767
1102	10-11	0.3366301559950955
1102	12-13	0.3650358750130209
1102	14-15	0.46757102066871425
1102	16-17	0.44830209173808555
1102	18-19	0.4560205065023837
1102	20-21	0.4586378552649464
1102	22-23	0.4233277988523554
1102	24-25	0.44582412952464523
1102	26-27	0.4874201637777631
1102	28-29	0.501288737393466
1102	30-31	0.472236075411125
1102	32-33	0.4775467873249113
1102	34-35	0.4922822073237043
1102	36-37	0.47712287817946475
1102	38-39	0.5106351065126589
1102	40-41	0.4936586129948708
1102	42-43	0.5121682540046422
1102	44-45	0.5005045051218175
1102	46-47	0.527797254666126
1102	48-49	0.5136124583757677
1102	50-51	0.5109306944427203
1102	52-53	0.5058815200231734
1102	54-55	0.4996044767821175
1102	56-57	0.5031724659553092
1102	58-59	0.49358857571075987
1102	60-61	0.4872222989090318
1102	62-63	0.5143750344472764
1102	64-65	0.5131163803753154
1102	66-67	0.4777359721616321
1102	68-69	0.4924388074255077
1102	70-71	0.49714142912394976
1102	72-73	0.4596337798216723
1102	74-75	0.45635640280823964
1102	76-77	0.45740878939611207
1102	78-79	0.4434378757549098
1102	80-81	0.42319700377859704
1102	82-83	0.4117838354988308
1102	84-85	0.4244348438557637
1102	86-87	0.3836796894989831
1102	88-89	0.42682480681862955
1102	90-91	0.4306307223088197
1102	92-93	0.4477777816443407
1102	94-95	0.4009459308639123
1102	96-97	0.42816595797633994
1102	98-99	0.436165319817448
1102	100	0.4182323040311502
1103	1	0.496666407780463
1103	2	0.3820034198990143
1103	3	0.34613327733801214
1103	4	0.2604304270657778
1103	5	0.24756822438124004
1103	6	0.23172103102083952
1103	7	0.2375580849918606
1103	8	0.23380282379243056
1103	9	0.28277279883383954
1103	10-11	0.3018620208017495
1103	12-13	0.30684439264730656
1103	14-15	0.40390045207546166
1103	16-17	0.40178259456758525
1103	18-19	0.39074696698611433
1103	20-21	0.3927037106944411
1103	22-23	0.3961106750220722
1103	24-25	0.3904543501796738
1103	26-27	0.42316224048711604
1103	28-29	0.44227117257217685
1103	30-31	0.429438518694937
1103	32-33	0.4435898690352005
1103	34-35	0.4563274944238813
1103	36-37	0.4387760941243215
1103	38-39	0.4577248829612941
1103	40-41	0.45804898376927383
1103	42-43	0.4606976420396123
1103	44-45	0.46099059147362453
1103	46-47	0.4362727763546914
1103	48-49	0.4437995273362034
1103	50-51	0.46481245906162627
1103	52-53	0.4381203866709811
1103	54-55	0.4411950571807708
1103	56-57	0.4213708907372151
1103	58-59	0.42008866246203524
1103	60-61	0.4341869391193285
1103	62-63	0.4514645022771049
1103	64-65	0.47492168848128813
1103	66-67	0.45359865349357875
1103	68-69	0.4386913344116934
1103	70-71	0.42432467923664063
1103	72-73	0.39883286624507264
1103	74-75	0.4349494809979504
1103	76-77	0.4043388673192112
1103	78-79	0.385755983312734
1103	80-81	0.38486090363682735
1103	82-83	0.39332936562036735
1103	84-85	0.37043745944417594
1103	86-87	0.3889547331390588
1103	88-89	0.38411789902490057
1103	90-91	0.38300619639683475
1103	92-93	0.39393874503280557
1103	94-95	0.3394117780314687
1103	96-97	0.39160325836389376
1103	98-99	0.3982583055163431
1103	100	0.4030298239210168
1104	1	0.41900524813480544
1104	2	0.29088773952668845
1104	3	0.26894896557158177
1104	4	0.2143177980991311
1104	5	0.17089124036497338
1104	6	0.17034143535053659
1104	7	0.17190021859229176
1104	8	0.17596672631091081
1104	9	0.21646722766341497
1104	10-11	0.2523897989167949
1104	12-13	0.2653337654074335
1104	14-15	0.3443323063907897
1104	16-17	0.3409720666060352
1104	18-19	0.3618351477697246
1104	20-21	0.3377582108994659
1104	22-23	0.33645952371918497
1104	24-25	0.3036989344323615
1104	26-27	0.34057135123816806
1104	28-29	0.3675858636686158
1104	30-31	0.35968858592834607
1104	32-33	0.3500025558394029
1104	34-35	0.35985908842642544
1104	36-37	0.34756345815032574
1104	38-39	0.34869432386372523
1104	40-41	0.3645271625221014
1104	42-43	0.3815460665749626
1104	44-45	0.4047526813810194
1104	46-47	0.3976078546398085
1104	48-49	0.3877530131109381
1104	50-51	0.4013734996079492
1104	52-53	0.3818084893542206
1104	54-55	0.3736704808853446
1104	56-57	0.3729407096535269
1104	58-59	0.41048897972806486
1104	60-61	0.4046753910631651
1104	62-63	0.4324610850754098
1104	64-65	0.3991740723952617
1104	66-67	0.40661961113877965
1104	68-69	0.40256122828103713
1104	70-71	0.3855295165091803
1104	72-73	0.3330695508466377
1104	74-75	0.3438774688119466
1104	76-77	0.32436033165116385
1104	78-79	0.31117424858699394
1104	80-81	0.28702320699986217
1104	82-83	0.33120358952850637
1104	84-85	0.2761736039826985
1104	86-87	0.30464620398608844
1104	88-89	0.32619212733813185
1104	90-91	0.31109844603190595
1104	92-93	0.2909141208152235
1104	94-95	0.2525238057442962
1104	96-97	0.2997881537521323
1104	98-99	0.31171793350443977
1104	100	0.29487714948674437
1105	1	0.3938713878918527
1105	2	0.29340864071573236
1105	3	0.24230629020633643
1105	4	0.17568639046636747
1105	5	0.16156574389390954
1105	6	0.17522214074857345
1105	7	0.17794103139961948
1105	8	0.14871352132308857
1105	9	0.20655039407682096
1105	10-11	0.18392187260657522
1105	12-13	0.2077653852760335
1105	14-15	0.28501080824294434
1105	16-17	0.28972853696090084
1105	18-19	0.3056556642039183
1105	20-21	0.2793477002096125
1105	22-23	0.27147307584704805
1105	24-25	0.28941595469405
1105	26-27	0.3284345053284454
1105	28-29	0.31460592112755137
1105	30-31	0.31101165163467215
1105	32-33	0.29090617928007134
1105	34-35	0.3128623778111006
1105	36-37	0.31352202494223746
1105	38-39	0.28224886841586994
1105	40-41	0.3025226258175806
1105	42-43	0.3372915982591209
1105	44-45	0.346757551251514
1105	46-47	0.34439437802240747
1105	48-49	0.3572557374352314
1105	50-51	0.3246479410190233
1105	52-53	0.3458584436501084
1105	54-55	0.3361536063546211
1105	56-57	0.32052601344059184
1105	58-59	0.31604931131371927
1105	60-61	0.3333306029336853
1105	62-63	0.32745465851807865
1105	64-65	0.31931497197575
1105	66-67	0.26701015469075884
1105	68-69	0.29658612361024694
1105	70-71	0.2889529646200373
1105	72-73	0.2498144757617169
1105	74-75	0.27659602342217227
1105	76-77	0.2686080710072787
1105	78-79	0.2332277174518822
1105	80-81	0.2312016216852868
1105	82-83	0.24066481665504824
1105	84-85	0.2309462930907813
1105	86-87	0.2366491894826126
1105	88-89	0.26246175993987464
1105	90-91	0.2724065249867138
1105	92-93	0.3083073320123262
1105	94-95	0.2567011296178592
1105	96-97	0.29752864030574955
1105	98-99	0.28182726337107056
1105	100	0.25440486925224093
1106	1	0.30414068722808807
1106	2	0.2215418794914541
1106	3	0.15869095725195592
1106	4	0.10616145444846836
1106	5	0.12876822689415945
1106	6	0.14046664283034005
1106	7	0.12108245878884105
1106	8	0.1551323062430754
1106	9	0.14679652476544902
1106	10-11	0.16877605553732167
1106	12-13	0.16048600924663958
1106	14-15	0.20556428487254408
1106	16-17	0.23083735092734514
1106	18-19	0.2371789613579196
1106	20-21	0.2421239255932548
1106	22-23	0.2499468591231846
1106	24-25	0.2469121273199164
1106	26-27	0.23626454598603175
1106	28-29	0.2589293900336145
1106	30-31	0.24922137721236481
1106	32-33	0.2298426861678493
1106	34-35	0.23187704479882854
1106	36-37	0.2628247585438146
1106	38-39	0.25450092504889454
1106	40-41	0.24944323615697783
1106	42-43	0.2459374017695879
1106	44-45	0.27514847359241656
1106	46-47	0.25768719386854144
1106	48-49	0.29365828846843556
1106	50-51	0.2499734214100826
1106	52-53	0.2694146679871565
1106	54-55	0.2310131240807891
1106	56-57	0.2717314729435074
1106	58-59	0.25393695930237214
1106	60-61	0.2408281053961403
1106	62-63	0.2604631413276266
1106	64-65	0.2663893726379172
1106	66-67	0.2702774615622232
1106	68-69	0.28322476409837094
1106	70-71	0.2521157446321567
1106	72-73	0.20398157556805074
1106	74-75	0.22344947340990018
1106	76-77	0.2131844470797688
1106	78-79	0.20451444698124988
1106	80-81	0.19325966634411884
1106	82-83	0.1949466682269403
1106	84-85	0.15305511973308228
1106	86-87	0.23256400768756436
1106	88-89	0.2326712612305002
1106	90-91	0.24215564473887952
1106	92-93	0.21906550696451177
1106	94-95	0.16719111985626967
1106	96-97	0.23026864849054363
1106	98-99	0.2094112002010462
1106	100	0.1996146829131007
1107	1	0.28176709531849653
1107	2	0.20893965739658427
1107	3	0.17935681792573632
1107	4	0.15400810395071574
1107	5	0.11757347907830962
1107	6	0.08956582004423552
1107	7	0.08962019696045331
1107	8	0.08357488351069264
1107	9	0.10481690201625327
1107	10-11	0.1223111821439744
1107	12-13	0.14385335221267326
1107	14-15	0.19616696184741755
1107	16-17	0.1713661137466005
1107	18-19	0.17457205431704637
1107	20-21	0.14475495570930974
1107	22-23	0.17821847578815664
1107	24-25	0.1929871900519018
1107	26-27	0.20003569019985434
1107	28-29	0.20178957587117452
1107	30-31	0.21855219614482024
1107	32-33	0.21678662606922927
1107	34-35	0.22903715414054915
1107	36-37	0.20670609199187595
1107	38-39	0.21577652339716735
1107	40-41	0.220195220058109
1107	42-43	0.22088137033762933
1107	44-45	0.22415482015636456
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2216	84-85	-0.6883916207469554
2216	86-87	-0.6564850648254605
2216	88-89	-0.6956327969119513
2216	90-91	-0.7167728976916834
2216	92-93	-0.7672407403165238
2216	94-95	-0.7372043503350696
2216	96-97	-0.7743612652237104
2216	98-99	-0.7828195830493385
2216	100	-0.8253832236736471
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12140.0
3	1897.0
4	1757.0
5	2499.0
6	4355.0
7	8573.0
8	16678.0
9	25026.0
10	32994.0
11	39945.0
12	45615.0
13	51430.0
14	56929.0
15	63058.0
16	68875.0
17	76090.0
18	83471.0
19	92914.0
20	103338.0
21	117788.0
22	137095.0
23	167181.0
24	212323.0
25	276502.0
26	368500.0
27	492347.0
28	652411.0
29	856631.0
30	1112347.0
31	1425669.0
32	1835982.0
33	2411136.0
34	3344170.0
35	5076459.0
36	8837203.0
37	1.5241062E7
38	1.7956579E7
39	5321161.0
40	12808.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	24.333313203960078	26.845582227292407	24.625831873402696	24.195272695344823
2	23.83951619900071	26.32244844907648	25.276019493618364	24.562015858304445
3	24.13111348722351	25.196437167881164	25.853966702368375	24.818482642526956
4	24.654244685310843	25.044944747183866	25.762743233198993	24.538067334306298
5	24.67050617403234	25.301724114000034	25.67806061907618	24.349709092891437
6	24.541526509697466	25.0519039262418	25.326598106062075	25.079971457998663
7	24.356756300269954	25.298468984065497	25.77304740076135	24.571727314903193
8	24.012422741626427	25.435797863533566	25.84321987725091	24.708559517589094
9	23.984245712576477	25.226653422752758	25.971785637662016	24.81731522700875
10-11	23.9673129356932	25.468405969736807	26.072474475840185	24.491806618729804
12-13	23.88513225592144	25.291399497206385	26.170215797422863	24.65325244944932
14-15	23.938792296922724	25.259437922053152	26.15109135311064	24.65067842791349
16-17	24.004325109436202	25.21024883386744	26.12301471462738	24.66241134206898
18-19	24.03532764416839	25.074281689081594	26.062021755403403	24.828368911346615
20-21	24.084323083114974	25.053966708370513	26.031903035247332	24.82980717326718
22-23	24.183007958022497	25.119434260236247	25.920598668684146	24.77695911305711
24-25	24.257807331363452	25.063985024189662	25.846989969139717	24.831217675307172
26-27	24.320492893035418	25.064592740494124	25.805614992544296	24.809299373926162
28-29	24.369395298868728	25.109434701093157	25.75327111178682	24.767898888251295
30-31	24.438875858786922	25.040049775898172	25.699795003788488	24.821279361526422
32-33	24.473712428464665	25.046986974073683	25.65007188008428	24.829228717377376
34-35	24.500547229775492	25.081926910245162	25.61494437715216	24.802581482827183
36-37	24.528260125890334	25.019897254321833	25.587228693371678	24.864613926416155
38-39	24.545045154647813	24.998668052627217	25.58012113432052	24.87616565840445
40-41	24.590070001296567	25.045581832202725	25.541246625962295	24.823101540538417
42-43	24.641624730091547	24.986728064520626	25.47727536584273	24.8943718395451
44-45	24.6486538121119	24.973452906580924	25.486865522891723	24.891027758415454
46-47	24.689036861957238	25.006846493141843	25.443465223089813	24.860651421811106
48-49	24.71796961622116	24.950232756817844	25.40004550533801	24.93175212162299
50-51	24.700825721855484	24.967608802338866	25.409124398052214	24.922441077753437
52-53	24.728563793338207	25.008119482529516	25.38800577616176	24.87531094797052
54-55	24.772281709124524	24.956934626522976	25.34545482046181	24.925328843890693
56-57	24.771523434819827	24.961759545220787	25.342302291643776	24.924414728315615
58-59	24.787966590000266	25.008281299254094	25.326092171717885	24.87765993902776
60-61	24.801594491647	24.962655830288863	25.286724700426976	24.949024977637162
62-63	24.811597943796475	24.96307090641809	25.29420587407816	24.931125275707284
64-65	24.845694077893143	25.01491530880586	25.259768709476766	24.87962190382423
66-67	24.861522187406095	24.96506299569679	25.231902750313363	24.94151206658375
68-69	24.86490581401236	24.96567987343171	25.229378310448297	24.94003600210763
70-71	24.90937500150055	25.005550536380788	25.192892768498908	24.892181693619754
72-73	24.918826318888314	24.993162813158236	25.166210489080417	24.921800378873034
74-75	24.929873508139384	24.990356214653055	25.176802731360702	24.902967545846863
76-77	24.950694894672274	25.02884509220631	25.154924389785172	24.865535623336246
78-79	24.95151758655436	25.0076969163049	25.12957210427303	24.911213392867708
80-81	24.953030362518472	25.012529875897638	25.14003708622683	24.894402675357057
82-83	24.96815997618204	25.062495174318776	25.12014487888899	24.84919997061019
84-85	24.97732222328035	25.027011697640077	25.089955399816006	24.90571067926357
86-87	24.97184176538243	25.039810487105267	25.095360143588163	24.892987603924137
88-89	25.009871400112754	25.075139763614313	25.073276753962816	24.84171208231012
90-91	25.013551913983566	25.048198567225576	25.04511721412428	24.89313230466658
92-93	24.996959519868632	25.06729811181906	25.05563155463269	24.880110813679618
94-95	25.0322415037121	25.10538347347746	25.02333192668965	24.839043096120786
96-97	25.03305864076068	25.095417438298796	25.00208369091575	24.86944023002477
98-99	25.0216467171134	25.123967990079322	25.00307920443081	24.851306088376468
100	25.059069167339697	25.155737353883172	24.969311103256796	24.815882375520335
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	28.0
1	26.0
2	23.5
3	24.0
4	24.0
5	32.5
6	40.5
7	43.5
8	58.0
9	78.0
10	96.0
11	141.5
12	238.0
13	362.5
14	554.5
15	975.0
16	1810.5
17	3344.0
18	6206.5
19	11142.5
20	18919.0
21	31012.5
22	48826.0
23	73139.0
24	105305.0
25	146489.5
26	197242.0
27	257627.0
28	327495.0
29	406599.0
30	494336.5
31	592577.0
32	701787.5
33	822920.5
34	955976.0
35	1099735.0
36	1257623.5
37	1436315.0
38	1630650.0
39	1825709.5
40	2003221.5
41	2145402.5
42	2241752.5
43	2288364.5
44	2291068.0
45	2250939.5
46	2169856.0
47	2074885.5
48	1984079.0
49	1897643.0
50	1827134.5
51	1776497.0
52	1739713.5
53	1728403.0
54	1747828.0
55	1785915.5
56	1829942.5
57	1858862.5
58	1857435.5
59	1825942.5
60	1769064.5
61	1688747.5
62	1588521.0
63	1468472.0
64	1329651.5
65	1176625.5
66	1021938.5
67	878735.0
68	746027.5
69	623058.0
70	513763.0
71	419698.0
72	339404.5
73	271139.0
74	215144.5
75	172277.0
76	138477.0
77	111581.5
78	90183.0
79	71429.5
80	56337.5
81	44917.5
82	35485.0
83	26991.0
84	20080.0
85	14604.5
86	10318.5
87	7120.0
88	4775.5
89	3003.0
90	1950.0
91	1252.5
92	648.5
93	362.5
94	206.5
95	106.5
96	61.0
97	39.0
98	35.5
99	89.0
100	139.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16006497192545743
2	0.0
3	0.0
4	0.0
5	2.805998739131219E-4
6	1.4855287442459394E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0022200401789008763
14-15	1.755624879563383E-4
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	3.6462978267854876E-4
32-33	0.0
34-35	0.0
36-37	0.0018088938395843233
38-39	0.0026882068134511114
40-41	0.002295066883155722
42-43	6.527323270171553E-4
44-45	7.352617016974852E-5
46-47	0.0017353676694145748
48-49	3.106105556150601E-4
50-51	3.278666975936745E-4
52-53	0.0011404059046736504
54-55	1.0503738595678359E-4
56-57	7.360119687400337E-4
58-59	4.7341850384807467E-4
60-61	7.502670425484543E-6
62-63	3.226148282958353E-5
64-65	0.0
66-67	1.9807049923279194E-4
68-69	0.0019266857652644306
70-71	0.0010953898821207432
72-73	4.8017090723101074E-5
74-75	0.002363341184027631
76-77	0.0011284016319928751
78-79	0.0014847784772033911
80-81	0.0018253997145203894
82-83	0.0011891732624393
84-85	7.727750538249079E-5
86-87	0.005194849002605498
88-89	0.005184345264009819
90-91	2.063234367008249E-4
92-93	0.03776844292188919
94-95	0.010170620028786846
96-97	5.649510830389861E-4
98-99	0.0035232540318075414
100	0.0023588395817723405
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6.6642938E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.08835760421515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.6589895569968	59.08944968594783
2	16.308375071504553	24.817549487686126
3	4.244208425166493	9.688045452026728
4	1.2068807540760151	3.673182976071227
5	0.37389678221863915	1.4224596036258585
6	0.11250811767827419	0.5136334734768988
7	0.04599203906173486	0.24496211005534196
8	0.01270917221556322	0.07736160323130635
9	0.0074880676568416715	0.05127792926744939
>10	0.02874006761923201	0.40927954286563584
>50	1.645093307942762E-4	0.008425191245913058
>100	4.743647503596348E-5	0.004372944499667242
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1554112455246196E-4	3.001068170193817E-6	7.502670425484543E-6	7.502670425484543E-6	0.0
2	1.1854219272265578E-4	7.502670425484543E-6	9.003204510581452E-6	1.5005340850969085E-5	1.5005340850969086E-6
3	1.230437949779465E-4	1.3504806765872178E-5	1.2004272680775268E-5	2.4008545361550537E-5	6.002136340387634E-6
4	1.2454432906304342E-4	2.250801127645363E-5	1.5005340850969085E-5	2.7009613531744355E-5	7.502670425484543E-6
5	1.3054646540343104E-4	2.5509079446647444E-5	1.5005340850969085E-5	3.601281804232581E-5	9.003204510581452E-6
6	1.3354753357362487E-4	3.3011749872131985E-5	1.5005340850969085E-5	4.501602255290726E-5	9.003204510581452E-6
7	1.4255073808420632E-4	3.4512283957228896E-5	1.5005340850969085E-5	5.7020295233682526E-5	9.003204510581452E-6
8	1.5305447667988468E-4	3.751335212742271E-5	1.5005340850969085E-5	6.602349974426397E-5	1.050373859567836E-5
9	1.5455501076498158E-4	3.901388621251962E-5	2.5509079446647444E-5	7.352617016974852E-5	1.6505874936065993E-5
10-11	1.6580901640320838E-4	4.651655663800416E-5	2.7009613531744355E-5	9.153257919091142E-5	1.8756676063711356E-5
12-13	1.7181115274359605E-4	6.077163044642479E-5	3.226148282958353E-5	1.2079299385030115E-4	2.1757744233905174E-5
14-15	1.7631275499888673E-4	8.02785735526846E-5	4.1264687340164985E-5	1.3579833470127024E-4	2.250801127645363E-5
16-17	1.8756676063711358E-4	9.453364736110523E-5	4.7266823680552615E-5	1.5530527780753002E-4	2.5509079446647444E-5
18-19	1.9957103331788882E-4	1.0653792004188052E-4	5.401922706348871E-5	1.7781328908398367E-4	2.8510147616841263E-5
20-21	2.235795786794394E-4	1.1629139159501041E-4	6.002136340387634E-5	1.9431916402004967E-4	3.3011749872131985E-5
22-23	2.408357206580538E-4	1.282956642757857E-4	6.752403382936087E-5	2.0782397078592183E-4	3.5262550999777355E-5
24-25	2.6184319784941057E-4	1.312967324459795E-4	7.352617016974852E-5	2.3108224910492394E-4	3.601281804232581E-5
26-27	2.8810254433860646E-4	1.3879940287146405E-4	7.727750538249079E-5	2.5208972629628065E-4	3.676308508487426E-5
28-29	3.1136082265760854E-4	1.4780260738204548E-4	8.102884059523307E-5	2.685956012323466E-4	3.751335212742271E-5
30-31	3.421217714020951E-4	1.6956035161595065E-4	8.402990876542687E-5	2.8810254433860646E-4	3.901388621251962E-5
32-33	3.8788806099755084E-4	1.8981756176475895E-4	8.628070989307224E-5	3.12111089700157E-4	4.201495438271344E-5
34-35	4.2014954382713434E-4	1.9581969810514657E-4	9.078231214836297E-5	3.3837043618935287E-4	4.276522142526189E-5
36-37	4.531612936992664E-4	2.085742378284703E-4	9.67844484887506E-5	3.6538004972109725E-4	4.351548846781035E-5
38-39	5.056799866776582E-4	2.2282931163689093E-4	9.903524961639597E-5	3.9464046438048695E-4	4.7266823680552615E-5
40-41	5.574484126135015E-4	2.3708438544531155E-4	1.0053578370149287E-4	4.171484756569406E-4	4.8017090723101074E-5
42-43	6.189703101024748E-4	2.490886581260868E-4	1.0428711891423515E-4	4.2765221425261895E-4	5.026789185074644E-5
44-45	7.112531563359347E-4	2.655945330621528E-4	1.147908575099135E-4	4.434078221461365E-4	5.326896002094026E-5
46-47	7.840290594631347E-4	2.836009420833157E-4	1.1704165863755886E-4	4.614142311672994E-4	5.702029523368252E-5
48-49	8.815637749944338E-4	3.0085708406193013E-4	1.2229352793539802E-4	4.7566930497572E-4	6.22721645315217E-5
50-51	0.0010098594392702195	3.2036402716818996E-4	1.245443290630434E-4	4.966767821670767E-4	6.452296565916707E-5
52-53	0.0011666652511628465	3.3912070323190136E-4	1.3504806765872178E-4	5.109318559754974E-4	7.127536904210315E-5
54-55	0.0013834924264593497	3.6012818042325807E-4	1.3579833470127024E-4	5.289382649966603E-4	7.87780394675877E-5
56-57	0.00160182013584095	3.7513352127422717E-4	1.410502039991094E-4	5.484452081029201E-4	8.327964172287844E-5
58-59	0.0018509087939670367	3.9013886212519626E-4	1.4405127216930323E-4	5.64951083038986E-4	9.003204510581452E-5
60-61	0.0021337594690078038	4.0064260072087456E-4	1.4405127216930323E-4	5.792061568474067E-4	9.52839144036537E-5
62-63	0.0024608758995589303	4.1864900974203747E-4	1.4405127216930323E-4	5.979628329111181E-4	9.82849825738475E-5
64-65	0.0028975313183221304	4.321538165079097E-4	1.4780260738204548E-4	6.107173726344417E-4	1.0428711891423515E-4
66-67	0.0033717000892127535	4.4640889031633034E-4	1.5305447667988468E-4	6.257227134854109E-4	1.1779192568010731E-4
68-69	0.003932149569996449	4.644152993374932E-4	1.5455501076498158E-4	6.407280543363799E-4	1.2154326089284959E-4
70-71	0.004585632164056152	4.884238446990438E-4	1.5830634597772383E-4	6.549831281448006E-4	1.230437949779465E-4
72-73	0.00538766763254045	5.019286514649159E-4	1.6130741414791767E-4	6.782414064638027E-4	1.230437949779465E-4
74-75	0.0063855227991298944	5.221858616137241E-4	1.6205768119046613E-4	6.992488836551594E-4	1.2379406202049496E-4
76-77	0.007627214754547586	5.469446740178232E-4	1.6205768119046613E-4	7.262584971869038E-4	1.2679513019068876E-4
78-79	0.009335572810430418	5.627002819113407E-4	1.6505874936065994E-4	7.435146391655182E-4	1.282956642757857E-4
80-81	0.011612633284564976	5.882093613579881E-4	1.6655928344575684E-4	7.630215822717779E-4	1.2904593131833413E-4
82-83	0.014816273556246876	6.099671055918933E-4	1.688100845734022E-4	7.870301276333286E-4	1.3054646540343104E-4
84-85	0.01935613943070757	6.257227134854109E-4	1.7331168682869293E-4	8.035360025693946E-4	1.3654860174381868E-4
86-87	0.025644127514306166	6.414783213789284E-4	1.740619538712414E-4	8.320461501862358E-4	1.433010051267548E-4
88	0.03182932901307563	6.512317929320583E-4	1.740619538712414E-4	8.538038944201409E-4	1.4405127216930323E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	11310	0.0	6.8162227	94
AGATCGG	15075	0.0	6.2364173	94
>>END_MODULE