FastQCFastQC Report
Wed 25 May 2016
SRR2049545_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049545_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69743960
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA474550.010.39907294
AGATCGG562650.09.748095594
ATCGGAA457100.09.43884294
TAGATCG183850.09.22849494
AGCGTCG175450.09.00063594
GCGTCGT157850.08.54524694
TCGGAAG493450.07.610109394
AAGAGCG354550.07.065403594
AGAGCGT283100.06.889623694
AAGATCG256000.06.792803394
CGTCGTG156800.06.7740794
GAGCGTC259900.06.65470594
CGGAAGA546600.06.62936694
GTCGTGT162300.06.312846794
CAGATCG268350.06.147418594
TCGTGTA141150.05.960180394
GAGATCG339700.05.132933694