##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049545_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 69743960 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.391675092724874 31.0 31.0 34.0 30.0 34.0 2 31.601649490507853 33.0 31.0 34.0 30.0 34.0 3 31.712275299538483 33.0 31.0 34.0 30.0 34.0 4 35.26302792385176 37.0 35.0 37.0 33.0 37.0 5 35.25047307322383 37.0 35.0 37.0 33.0 37.0 6 35.240762827347346 37.0 35.0 37.0 33.0 37.0 7 35.25763666129655 37.0 35.0 37.0 33.0 37.0 8 35.28225196848587 37.0 35.0 37.0 33.0 37.0 9 36.90728923336157 39.0 37.0 39.0 33.0 39.0 10-11 36.88808450939695 39.0 37.0 39.0 33.0 39.0 12-13 36.865353652129876 39.0 37.0 39.0 33.0 39.0 14-15 38.135672565767706 40.0 38.0 41.0 33.0 41.0 16-17 38.0878209740313 40.0 38.0 41.0 33.0 41.0 18-19 38.107785699292094 40.0 38.0 41.0 33.0 41.0 20-21 38.08903816187093 40.0 38.0 41.0 33.0 41.0 22-23 38.01829345508916 40.0 38.0 41.0 33.0 41.0 24-25 38.004539439687676 40.0 38.0 41.0 33.0 41.0 26-27 37.93307096413797 40.0 38.0 41.0 33.0 41.0 28-29 37.860602466507494 40.0 38.0 41.0 33.0 41.0 30-31 37.80051488329599 40.0 38.0 41.0 33.0 41.0 32-33 37.72345635378318 40.0 38.0 41.0 33.0 41.0 34-35 37.626102231648446 40.0 38.0 41.0 32.0 41.0 36-37 37.56601082731752 40.0 38.0 41.0 32.0 41.0 38-39 37.47428627511257 40.0 37.0 41.0 32.0 41.0 40-41 37.38120310346588 40.0 37.0 41.0 32.0 41.0 42-43 37.24696445398283 40.0 37.0 41.0 31.5 41.0 44-45 37.11048170336184 40.0 37.0 41.0 31.0 41.0 46-47 36.986709917245875 40.0 36.0 41.0 31.0 41.0 48-49 36.87199935306226 40.0 36.0 41.0 31.0 41.0 50-51 36.72394251057726 39.5 36.0 41.0 31.0 41.0 52-53 36.559054189065264 39.0 35.0 41.0 30.0 41.0 54-55 36.38655498626691 39.0 35.0 41.0 30.0 41.0 56-57 36.185830041769925 39.0 35.0 41.0 30.0 41.0 58-59 35.97251742659866 39.0 35.0 41.0 30.0 41.0 60-61 35.74960827432225 38.0 35.0 40.0 29.0 41.0 62-63 35.50456605131111 38.0 35.0 40.0 29.0 41.0 64-65 35.25896074011284 37.0 34.0 40.0 29.0 41.0 66-67 34.98796311537228 37.0 34.0 40.0 29.0 41.0 68-69 34.68626177808085 36.0 34.0 39.5 28.0 41.0 70-71 34.39362174875072 36.0 34.0 39.0 28.0 41.0 72-73 34.06699426014812 35.0 34.0 39.0 28.0 40.5 74-75 33.749700776956175 35.0 34.0 38.0 28.0 40.0 76-77 33.44079643599245 35.0 33.5 37.0 27.0 39.0 78-79 33.12261780088197 35.0 33.0 37.0 27.0 39.0 80-81 32.836841713605025 35.0 33.0 36.0 27.0 39.0 82-83 32.558593805112295 35.0 33.0 36.0 26.0 37.0 84-85 32.29883022845276 35.0 33.0 35.5 26.0 37.0 86-87 32.09952049611178 35.0 33.0 35.0 26.0 37.0 88-89 31.91313204756369 35.0 33.0 35.0 26.0 36.0 90-91 31.75548337088975 35.0 33.0 35.0 25.5 36.0 92-93 31.586269979507904 35.0 33.0 35.0 25.0 36.0 94-95 31.41433643142718 35.0 33.0 35.0 25.0 35.5 96-97 31.261177412352268 35.0 33.0 35.0 24.5 35.0 98-99 31.12444046767634 35.0 33.0 35.0 24.0 35.0 100 31.008100414716917 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5513276958188804 1101 2 0.5142676407507274 1101 3 0.5177547333070898 1101 4 0.4511064701096785 1101 5 0.4364946875224547 1101 6 0.4893011484752279 1101 7 0.4861082719338441 1101 8 0.4722328244058076 1101 9 0.5517881642333364 1101 10-11 0.5536753684067364 1101 12-13 0.5760974541190365 1101 14-15 0.6975086541088089 1101 16-17 0.7254668947255354 1101 18-19 0.7043506211984507 1101 20-21 0.707668129809889 1101 22-23 0.7290957451168723 1101 24-25 0.7100659586225717 1101 26-27 0.7070676976585091 1101 28-29 0.7131563607705331 1101 30-31 0.7086228284332492 1101 32-33 0.7182957549091142 1101 34-35 0.7414941207391976 1101 36-37 0.7197049862805969 1101 38-39 0.7320468097169623 1101 40-41 0.7526736216078618 1101 42-43 0.7355675955418306 1101 44-45 0.7272831086006022 1101 46-47 0.7080489222849238 1101 48-49 0.7219357130799935 1101 50-51 0.7343302167723209 1101 52-53 0.7073309077143293 1101 54-55 0.6955790221279585 1101 56-57 0.7154346703283565 1101 58-59 0.6941287701438412 1101 60-61 0.7242771763093074 1101 62-63 0.6985638299270462 1101 64-65 0.7262694882682155 1101 66-67 0.7025748392620201 1101 68-69 0.6882244890030549 1101 70-71 0.7041623035284843 1101 72-73 0.6913902940637726 1101 74-75 0.6720485515587313 1101 76-77 0.6626139943911866 1101 78-79 0.6581645525757267 1101 80-81 0.6525206057776103 1101 82-83 0.6931397326082944 1101 84-85 0.6863170434944053 1101 86-87 0.6235602917305272 1101 88-89 0.639403396854604 1101 90-91 0.6367352723733894 1101 92-93 0.6474596069946124 1101 94-95 0.6804254654429442 1101 96-97 0.6451858302881099 1101 98-99 0.6643434502795635 1101 100 0.6415912572282387 1102 1 0.4580634508316628 1102 2 0.4308955322133379 1102 3 0.42341304132098045 1102 4 0.39330843793965187 1102 5 0.3601010880448072 1102 6 0.3954205468067471 1102 7 0.4084557139958278 1102 8 0.40215439821813703 1102 9 0.4955865861815596 1102 10-11 0.5100144815478131 1102 12-13 0.5049600710707836 1102 14-15 0.6215514186153683 1102 16-17 0.6273331139248199 1102 18-19 0.5837749754025765 1102 20-21 0.6524744214708988 1102 22-23 0.6697030096967396 1102 24-25 0.6204814132122749 1102 26-27 0.6138403845396283 1102 28-29 0.6529803811452268 1102 30-31 0.6350957606871006 1102 32-33 0.6126295640866104 1102 34-35 0.6214161947298891 1102 36-37 0.6270987675758946 1102 38-39 0.650434072183451 1102 40-41 0.6152570682962661 1102 42-43 0.5929110686116488 1102 44-45 0.6142146348528144 1102 46-47 0.6260883393520373 1102 48-49 0.6142942224114023 1102 50-51 0.6530515280312486 1102 52-53 0.6327655080881485 1102 54-55 0.6084884773817691 1102 56-57 0.6476909672594005 1102 58-59 0.6586065371056904 1102 60-61 0.6589108234568073 1102 62-63 0.5989097770995286 1102 64-65 0.5984546669565063 1102 66-67 0.618708083462316 1102 68-69 0.6112267626441792 1102 70-71 0.6334233921731638 1102 72-73 0.6272380464021694 1102 74-75 0.5868964409401514 1102 76-77 0.6003312846403759 1102 78-79 0.5922313762470282 1102 80-81 0.5882431399494763 1102 82-83 0.6114328585674969 1102 84-85 0.6347611411970249 1102 86-87 0.6202480483371531 1102 88-89 0.6275790214410506 1102 90-91 0.5929874442839456 1102 92-93 0.6081043134979431 1102 94-95 0.636161977966232 1102 96-97 0.6170089060548598 1102 98-99 0.6067328749505556 1102 100 0.6088321494703557 1103 1 0.3610680847920342 1103 2 0.3207142719048903 1103 3 0.3229073909590774 1103 4 0.2910480807471174 1103 5 0.26972688652906385 1103 6 0.3123260162338326 1103 7 0.32197414675837166 1103 8 0.28937485857480283 1103 9 0.3928306704373057 1103 10-11 0.3928771970726217 1103 12-13 0.40076390121592453 1103 14-15 0.5056476227113791 1103 16-17 0.5279926062799163 1103 18-19 0.4917616561067746 1103 20-21 0.4845553192128307 1103 22-23 0.5215459711305428 1103 24-25 0.4819549464649171 1103 26-27 0.47668842750509555 1103 28-29 0.5185873429911894 1103 30-31 0.5073914596933093 1103 32-33 0.5085521252779444 1103 34-35 0.5429294351522458 1103 36-37 0.5058216914801434 1103 38-39 0.5255809517215226 1103 40-41 0.5299570474756976 1103 42-43 0.5116449881200822 1103 44-45 0.5195244264503316 1103 46-47 0.5181645962337669 1103 48-49 0.5201035711817852 1103 50-51 0.5159136182861488 1103 52-53 0.5169522355621936 1103 54-55 0.5188502055395361 1103 56-57 0.5458038548966542 1103 58-59 0.5643595347923949 1103 60-61 0.567685482923217 1103 62-63 0.5310576004644005 1103 64-65 0.5439088892860866 1103 66-67 0.5399376425544489 1103 68-69 0.5369170987955769 1103 70-71 0.5425858174568248 1103 72-73 0.5274614078817521 1103 74-75 0.5012492228719267 1103 76-77 0.5134674493589912 1103 78-79 0.5268958346314321 1103 80-81 0.489861266953028 1103 82-83 0.5338973958342805 1103 84-85 0.5344342727566058 1103 86-87 0.5282009949536288 1103 88-89 0.5184565469925388 1103 90-91 0.526166826312096 1103 92-93 0.5255863439557054 1103 94-95 0.549093709338905 1103 96-97 0.5351402348400036 1103 98-99 0.49560739939634146 1103 100 0.44123137280920943 1104 1 0.2808692923148506 1104 2 0.27667333357884516 1104 3 0.28298832122527884 1104 4 0.2371628813563902 1104 5 0.2434615868641572 1104 6 0.2876697113440585 1104 7 0.2850765643029476 1104 8 0.2943697842190076 1104 9 0.3722620773649794 1104 10-11 0.343389256657602 1104 12-13 0.3848057513630252 1104 14-15 0.454656208722902 1104 16-17 0.48177102442212316 1104 18-19 0.4597274174408952 1104 20-21 0.49707906293510007 1104 22-23 0.5176905098948694 1104 24-25 0.48237901416511875 1104 26-27 0.4832044642246771 1104 28-29 0.4808274067041012 1104 30-31 0.472987801357732 1104 32-33 0.4568403888050412 1104 34-35 0.4648481810901046 1104 36-37 0.4987782544641348 1104 38-39 0.4362920239275425 1104 40-41 0.415648033960224 1104 42-43 0.43615645570381645 1104 44-45 0.4338687015382803 1104 46-47 0.4253495686153457 1104 48-49 0.42510610425279083 1104 50-51 0.45318992181803 1104 52-53 0.45745159373684174 1104 54-55 0.4613776982926936 1104 56-57 0.47123058223807135 1104 58-59 0.4707659180722601 1104 60-61 0.4672864581374796 1104 62-63 0.45205475563239617 1104 64-65 0.4395732894075124 1104 66-67 0.4454731318845262 1104 68-69 0.46536939894271256 1104 70-71 0.4860617100371414 1104 72-73 0.4745652131265885 1104 74-75 0.4332302898665361 1104 76-77 0.46263544195461037 1104 78-79 0.4521008789926526 1104 80-81 0.3986391825963551 1104 82-83 0.4081749737475917 1104 84-85 0.4142508325897012 1104 86-87 0.40948435796267546 1104 88-89 0.40595377055419135 1104 90-91 0.40636260450838435 1104 92-93 0.4292308549043433 1104 94-95 0.40293852741476854 1104 96-97 0.3686393625764879 1104 98-99 0.3742294251401681 1104 100 0.4136536954195762 1105 1 0.26219851201324573 1105 2 0.23480594835956836 1105 3 0.2515186033971588 1105 4 0.2479562421783541 1105 5 0.21309525291777476 1105 6 0.28159623380047805 1105 7 0.2878281939052485 1105 8 0.2665950521989231 1105 9 0.2917805321915594 1105 10-11 0.32200872175508266 1105 12-13 0.31950089197737697 1105 14-15 0.3827769455674783 1105 16-17 0.4090765824020153 1105 18-19 0.39902668145872155 1105 20-21 0.38840099574396447 1105 22-23 0.4317109835646775 1105 24-25 0.39539246684226725 1105 26-27 0.4128274455305885 1105 28-29 0.4141769322877167 1105 30-31 0.3874985361668024 1105 32-33 0.3799764287072449 1105 34-35 0.4149440571842291 1105 36-37 0.42179209905032167 1105 38-39 0.4058089706113819 1105 40-41 0.4090504080294224 1105 42-43 0.38976641993571803 1105 44-45 0.36360952594437634 1105 46-47 0.35767754122702655 1105 48-49 0.35609185326393344 1105 50-51 0.36672889887774573 1105 52-53 0.3938455250158839 1105 54-55 0.41781669105226626 1105 56-57 0.41417279965495624 1105 58-59 0.41151932810237213 1105 60-61 0.41557804286263433 1105 62-63 0.38759466776281926 1105 64-65 0.37964085830179783 1105 66-67 0.42576244729074375 1105 68-69 0.43654514904332586 1105 70-71 0.4190490579072801 1105 72-73 0.39804261268793795 1105 74-75 0.38349081691081466 1105 76-77 0.38526473727317523 1105 78-79 0.3688225441950834 1105 80-81 0.37243239622125657 1105 82-83 0.4092490955142196 1105 84-85 0.39958869068892255 1105 86-87 0.3886092060016253 1105 88-89 0.3532294546738939 1105 90-91 0.36754333734781497 1105 92-93 0.40181802613181006 1105 94-95 0.4149687573495804 1105 96-97 0.4048196655561256 1105 98-99 0.39678222531928853 1105 100 0.37277224187378266 1106 1 0.21881709158162366 1106 2 0.19344531155763534 1106 3 0.1643389819563481 1106 4 0.1702267712926897 1106 5 0.1430712663484215 1106 6 0.22109552657758513 1106 7 0.22881262306030692 1106 8 0.21410899720682153 1106 9 0.2501710110234896 1106 10-11 0.269179648429855 1106 12-13 0.2782969137543745 1106 14-15 0.3243087347335063 1106 16-17 0.3699278522032259 1106 18-19 0.33413543200015283 1106 20-21 0.3257949475315485 1106 22-23 0.3006756898149021 1106 24-25 0.30839176824188996 1106 26-27 0.31552864389688295 1106 28-29 0.3540402494589614 1106 30-31 0.3347149675877219 1106 32-33 0.3315837201970524 1106 34-35 0.32874501733992645 1106 36-37 0.2789769066794108 1106 38-39 0.321424127523791 1106 40-41 0.3253726944313726 1106 42-43 0.2936494718445246 1106 44-45 0.3264033608257577 1106 46-47 0.3295013766701942 1106 48-49 0.3162605046553608 1106 50-51 0.31752192614520425 1106 52-53 0.34442883031172045 1106 54-55 0.3646268926601266 1106 56-57 0.33791548754754075 1106 58-59 0.3714977478994044 1106 60-61 0.3741803122381171 1106 62-63 0.3613545138981564 1106 64-65 0.3471172980332611 1106 66-67 0.36162340947067406 1106 68-69 0.33000662930285785 1106 70-71 0.3314018739898401 1106 72-73 0.3263889157592459 1106 74-75 0.2999587196218627 1106 76-77 0.3166727336805977 1106 78-79 0.26592093247514015 1106 80-81 0.3104514248827144 1106 82-83 0.3135713092731294 1106 84-85 0.3322449940471657 1106 86-87 0.3108119228747199 1106 88-89 0.31099829126699774 1106 90-91 0.2682810122331567 1106 92-93 0.34223231560516965 1106 94-95 0.31284078784455005 1106 96-97 0.27541362223709953 1106 98-99 0.2606542232955249 1106 100 0.2542661053677122 1107 1 0.18521806190955203 1107 2 0.16799004747270274 1107 3 0.16998816899984703 1107 4 0.15618968416197987 1107 5 0.12536618973348368 1107 6 0.12045585756750654 1107 7 0.1664781885605109 1107 8 0.1418730607469172 1107 9 0.19773339183977612 1107 10-11 0.2008927361445103 1107 12-13 0.21007499822292885 1107 14-15 0.24007042933519074 1107 16-17 0.25104693476088613 1107 18-19 0.24074134627150556 1107 20-21 0.23100304056988108 1107 22-23 0.24471274319522252 1107 24-25 0.21511498121455475 1107 26-27 0.258564420665202 1107 28-29 0.2672578327960906 1107 30-31 0.2069085840249727 1107 32-33 0.2337458191368711 1107 34-35 0.1902000435057971 1107 36-37 0.22539899656056406 1107 38-39 0.22952955690514187 1107 40-41 0.2408806711169973 1107 42-43 0.23797174821141454 1107 44-45 0.26256546496229305 1107 46-47 0.24278489810051695 1107 48-49 0.2521706046612877 1107 50-51 0.20913835782866386 1107 52-53 0.2328305047942152 1107 54-55 0.22691108808149352 1107 56-57 0.2536474501744763 1107 58-59 0.23107849420890858 1107 60-61 0.29270493953002585 1107 62-63 0.2840217170689172 1107 64-65 0.26881401680912376 1107 66-67 0.2738431597240023 1107 68-69 0.2604696941053817 1107 70-71 0.23996142573270873 1107 72-73 0.25182504323223753 1107 74-75 0.26908737876068756 1107 76-77 0.2758966145296924 1107 78-79 0.28742723762685074 1107 80-81 0.2802730423544517 1107 82-83 0.2599140244521223 1107 84-85 0.2594793806722535 1107 86-87 0.2517439898651972 1107 88-89 0.24436550406015556 1107 90-91 0.21453838538697667 1107 92-93 0.25043986180246236 1107 94-95 0.2686614717720417 1107 96-97 0.23373227104436367 1107 98-99 0.2090889728770975 1107 100 0.22429188388680998 1108 1 0.11272110314560635 1108 2 0.07221813169583768 1108 3 0.1033157829010456 1108 4 0.09181061030292881 1108 5 0.07804715260525086 1108 6 0.1237545227362915 1108 7 0.141130638456616 1108 8 0.12075949969391075 1108 9 0.08819613213913158 1108 10-11 0.11691913115882357 1108 12-13 0.1376355912448517 1108 14-15 0.17860141048859646 1108 16-17 0.15823284985783914 1108 18-19 0.14926469084797844 1108 20-21 0.17228193249373192 1108 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-0.679137815581484 2215 48-49 -0.6988024904901948 2215 50-51 -0.7586301744023984 2215 52-53 -0.726678508406529 2215 54-55 -0.6733830583091702 2215 56-57 -0.7422129237737991 2215 58-59 -0.733289794297427 2215 60-61 -0.7572592202409396 2215 62-63 -0.6930246704994261 2215 64-65 -0.7300580040847038 2215 66-67 -0.7271211194422165 2215 68-69 -0.7668956707191796 2215 70-71 -0.7166494682681233 2215 72-73 -0.6959140922291809 2215 74-75 -0.7365597623320284 2215 76-77 -0.7427683525730515 2215 78-79 -0.6296043643067293 2215 80-81 -0.6693912344081525 2215 82-83 -0.6235540546494391 2215 84-85 -0.5914128064988091 2215 86-87 -0.6366801121092678 2215 88-89 -0.6515691714720617 2215 90-91 -0.6584597126511476 2215 92-93 -0.671713133351524 2215 94-95 -0.6427732618140674 2215 96-97 -0.6287027263963125 2215 98-99 -0.6898126446412682 2215 100 -0.7258399473780841 2216 1 -0.5652048596979498 2216 2 -0.5379183986121667 2216 3 -0.521639726531383 2216 4 -0.4736708120943405 2216 5 -0.4469408483559434 2216 6 -0.6043863056465568 2216 7 -0.6234171895928426 2216 8 -0.6148587436062698 2216 9 -0.7305571749506186 2216 10-11 -0.7268073992722179 2216 12-13 -0.7682216247769986 2216 14-15 -0.8823799411827054 2216 16-17 -0.9597889038662686 2216 18-19 -0.9470342053009801 2216 20-21 -0.9414692980448649 2216 22-23 -0.9194094532985488 2216 24-25 -0.9462314947692079 2216 26-27 -0.9789417513972936 2216 28-29 -1.0972646586112162 2216 30-31 -0.9528421109537959 2216 32-33 -0.9947739094800241 2216 34-35 -0.9469598239043222 2216 36-37 -0.9580130132104614 2216 38-39 -0.9092641244568043 2216 40-41 -0.9474034691679165 2216 42-43 -0.8969491753200671 2216 44-45 -0.8561666424699794 2216 46-47 -0.8676791396458796 2216 48-49 -0.9314731726736554 2216 50-51 -0.9170838948918458 2216 52-53 -1.0309737468724052 2216 54-55 -1.0338987302104528 2216 56-57 -1.0739495940589947 2216 58-59 -1.020504771660157 2216 60-61 -1.0624655755331673 2216 62-63 -1.0062199014604616 2216 64-65 -0.9151253611168713 2216 66-67 -0.8975165178898763 2216 68-69 -0.9853182848780477 2216 70-71 -0.9752630028746765 2216 72-73 -0.9425345517931092 2216 74-75 -0.9221400120634797 2216 76-77 -0.8884401645539768 2216 78-79 -0.8596940487908853 2216 80-81 -0.9116394374398453 2216 82-83 -0.9548088130843446 2216 84-85 -1.1186464517337669 2216 86-87 -1.1030948834734247 2216 88-89 -1.0290443731869772 2216 90-91 -1.014953041828317 2216 92-93 -0.9988208664199085 2216 94-95 -1.023790043939556 2216 96-97 -1.0007285551080436 2216 98-99 -1.0540257990462827 2216 100 -1.0461595624779427 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 590719.0 3 121789.0 4 91120.0 5 80686.0 6 81930.0 7 83790.0 8 86151.0 9 99168.0 10 108928.0 11 119629.0 12 124594.0 13 130911.0 14 134525.0 15 138480.0 16 142432.0 17 145892.0 18 151459.0 19 156698.0 20 163817.0 21 174786.0 22 194028.0 23 225971.0 24 276796.0 25 347906.0 26 441460.0 27 562430.0 28 711789.0 29 884015.0 30 1116823.0 31 1430271.0 32 1864432.0 33 2488650.0 34 3496840.0 35 5277961.0 36 8840859.0 37 1.4744409E7 38 1.8268101E7 39 5631139.0 40 12576.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.750532031971083 27.939635032195437 25.659472387649046 23.650360548184434 2 23.12984519584571 27.54641393688338 26.172310629083572 23.151430238187334 3 23.596325861948543 25.805119881988187 26.178564132537936 24.419990123525334 4 23.498719159134147 25.74555741416446 26.3595603384372 24.39616308826419 5 23.717674950491407 26.248205518492355 26.552458755613635 23.481660775402602 6 23.983944973495618 26.084950457615015 25.78562763328462 24.14547693560475 7 23.902846669883665 26.1008725352912 26.45966678389624 23.536614010928897 8 23.360220338365295 26.47693802682138 26.508023196714326 23.654818438099 9 23.220759251970968 26.190878570931226 26.697018526029993 23.891343651067814 10-11 23.27222132244496 26.36374832319853 26.71265787683903 23.651372477517484 12-13 23.267659505163973 26.195230661772445 26.814902434830973 23.72220739823261 14-15 23.32991016687897 26.150030638638107 26.819192181207207 23.70086701327572 16-17 23.37605147241833 26.05527289727579 26.797261703620475 23.771413926685412 18-19 23.429416566733853 25.90063925914956 26.704139403643357 23.965804770473234 20-21 23.47071771938695 25.86183797256935 26.659726750410684 24.00771755763301 22-23 23.56581604220144 25.876875465921323 26.567818404908085 23.98949008696916 24-25 23.630969618191834 25.825672755618147 26.44860400500647 24.09475362118355 26-27 23.66702442708202 25.840382889878555 26.400593059273795 24.091999623765624 28-29 23.706955294185178 25.885266973877513 26.350206730536385 24.057571001400923 30-31 23.759633842078433 25.79638175656378 26.304432143095486 24.139552258262306 32-33 23.80922852195287 25.80974725995535 26.250518173065156 24.130506045026618 34-35 23.828046875211893 25.849518754982498 26.215609460426375 24.106824909379238 36-37 23.84057769480778 25.783898264343357 26.185892885070338 24.189631155778518 38-39 23.84460434628132 25.764612503420487 26.203569630973767 24.18721351932443 40-41 23.87359656056301 25.798085645586426 26.172627944989337 24.155689848861233 42-43 23.92603140244031 25.727502787617794 26.098330094258465 24.24813571568343 44-45 23.93592208814455 25.720735928850885 26.111463042310806 24.231878940693754 46-47 23.97900240549983 25.75779602949431 26.061354785059855 24.20184677994601 48-49 23.97714290486796 25.684973130185913 26.045737624357386 24.292146340588744 50-51 23.977311096252073 25.71342023656962 26.046266072810702 24.263002594367606 52-53 23.993827543070648 25.742098814033575 26.019377018110497 24.244696624785284 54-55 24.038220246157056 25.703601956318288 25.970341904449647 24.28783589307501 56-57 24.022862304252826 25.716982053898068 25.968577744252418 24.291577897596685 58-59 24.035343332119155 25.756968364813254 25.962339225024866 24.24534907804273 60-61 24.06996763972411 25.723223530231248 25.896384578695407 24.310424251349236 62-63 24.068138811601298 25.708482796616355 25.915127289563582 24.308251102218765 64-65 24.082858683363543 25.76688801872738 25.890735022711336 24.259518275197745 66-67 24.115474093544137 25.717643136417266 25.84617979188578 24.320702978152816 68-69 24.10133503364899 25.730873018678057 25.855369710113287 24.31242223755967 70-71 24.147413485978188 25.772824516552156 25.818187334100045 24.261574663369615 72-73 24.17308359462642 25.74520992612616 25.78577811352748 24.295928365719945 74-75 24.172580962831297 25.76958339374701 25.791463659129953 24.26637198429174 76-77 24.20316433828001 25.80854307470463 25.766351180051977 24.22194140696338 78-79 24.239499564332117 25.78306646029049 25.72912488421124 24.24830909116615 80-81 24.22614305152126 25.805758400212746 25.738162582432473 24.22993596583352 82-83 24.24370418033936 25.8607091286842 25.718526158297305 24.177060532679135 84-85 24.260824555119772 25.82879834902548 25.67024943453155 24.240127661323204 86-87 24.26319825702008 25.85091363998776 25.67717518955601 24.20871291343615 88-89 24.299331424369107 25.894186255687174 25.641569743280552 24.16491257666317 90-91 24.336380358003574 25.87706325730566 25.60142672713786 24.18512965755291 92-93 24.336938935652412 25.91583114016831 25.588214887110322 24.159015037068958 94-95 24.38169559924156 25.98008515485999 25.535520394960876 24.10269885093758 96-97 24.43871513249665 25.982107124736803 25.468099221544744 24.111078521221803 98-99 24.474308503243876 26.023154634561052 25.451353619012494 24.051183243182575 100 24.54376577768421 26.078584045776825 25.385229350215422 23.992420826323542 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1611.0 1 3209.5 2 6303.5 3 8061.5 4 7365.0 5 5616.5 6 4430.5 7 3718.0 8 3199.5 9 2807.0 10 2567.0 11 2462.5 12 2512.0 13 2799.5 14 3343.0 15 4427.5 16 6387.0 17 9582.0 18 15271.5 19 24471.5 20 38274.0 21 57962.0 22 85124.5 23 120752.5 24 166513.0 25 223710.0 26 291791.0 27 371233.0 28 462915.0 29 565018.5 30 675055.5 31 796254.0 32 926976.5 33 1069736.5 34 1223820.5 35 1385713.5 36 1563108.0 37 1760369.5 38 1968311.0 39 2171238.0 40 2348204.5 41 2478784.0 42 2547691.5 43 2557915.0 44 2519610.5 45 2434891.5 46 2312406.0 47 2173034.0 48 2038846.5 49 1920797.5 50 1823307.0 51 1744402.0 52 1685214.0 53 1651879.5 54 1647169.0 55 1662339.0 56 1684048.5 57 1694141.0 58 1678045.0 59 1636802.5 60 1575870.5 61 1497146.0 62 1404572.5 63 1295772.0 64 1172765.0 65 1043020.0 66 911130.5 67 787830.5 68 673323.5 69 565705.5 70 472385.5 71 390863.5 72 319359.0 73 259304.0 74 208878.5 75 169666.5 76 139407.5 77 115651.0 78 95484.0 79 77166.0 80 62237.5 81 50703.5 82 41451.5 83 33258.5 84 25896.5 85 19511.5 86 14697.0 87 10908.0 88 7800.0 89 5587.5 90 4098.5 91 2934.0 92 1931.0 93 1266.5 94 882.5 95 622.0 96 463.0 97 342.0 98 267.0 99 239.5 100 463.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0849363873230026 2 0.034962167333199894 3 0.006453605444829917 4 0.00606073988342503 5 0.10426852733914163 6 0.15692254927881927 7 0.07405659214073877 8 0.07854443596262672 9 0.06545226281960474 10-11 0.0506853926849006 12-13 0.04248970090026434 14-15 0.05662282439941752 16-17 0.0793975564335607 18-19 0.06518987450669562 20-21 0.06967556760470728 22-23 0.06888840266597997 24-25 0.08341941008224942 26-27 0.09937132907279712 28-29 0.08292474359069947 30-31 0.07308303113273178 32-33 0.06590176410975229 34-35 0.08426679528951324 36-37 0.08737387438281394 38-39 0.0898536590121926 40-41 0.0823562355793964 42-43 0.08782767712071411 44-45 0.09763426108870216 46-47 0.10197585568700142 48-49 0.08483100185306369 50-51 0.0906207505280744 52-53 0.09375077067605567 54-55 0.1018640180454336 56-57 0.10132490326044004 58-59 0.09444043613239053 60-61 0.08435282424456542 62-63 0.08468690335335131 64-65 0.09933906821465258 66-67 0.11160894792896761 68-69 0.10125966463619215 70-71 0.08794955147370469 72-73 0.09139931257129649 74-75 0.11247282201928309 76-77 0.09446696172686495 78-79 0.10762939184984621 80-81 0.08802841134916915 82-83 0.09986886319618216 84-85 0.10968835150742802 86-87 0.0887790139819993 88-89 0.08216338733848781 90-91 0.09406549326995484 92-93 0.11577991843308008 94-95 0.08936042633656019 96-97 0.08325667197560907 98-99 0.09244599819109783 100 0.1122935950295911 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6.974396E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.77663350465387 #Duplication Level Percentage of deduplicated Percentage of total 1 75.94309388600301 56.02825804837165 2 16.843399366114053 24.852986040126318 3 4.817920878942967 10.663499488205854 4 1.5312569794053688 4.518839398841329 5 0.5208378443356954 1.9212831378454283 6 0.18511115833142391 0.8194126851503651 7 0.08259694334753702 0.4265607092569128 8 0.025526165261304076 0.15065876314099666 9 0.012109966070381629 0.0804089275675505 >10 0.03751575688010973 0.5053436743993296 >50 5.628720614176201E-4 0.026146874458839616 >100 6.818324674357945E-5 0.006602252635334194 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 9.319803463984551E-5 7.169079587680424E-6 2.8676318350721696E-6 4.301447752608255E-6 4.301447752608255E-6 2 9.749948239245376E-5 1.1470527340288678E-5 4.301447752608255E-6 1.2904343257824764E-5 7.169079587680424E-6 3 1.0036711422752594E-4 1.720579101043302E-5 1.0036711422752594E-5 2.2941054680577357E-5 7.169079587680424E-6 4 1.0180093014506203E-4 2.0073422845505188E-5 1.0036711422752594E-5 3.297776610332995E-5 8.60289550521651E-6 5 1.1040382565027853E-4 2.437487059811344E-5 1.1470527340288678E-5 4.0146845691010376E-5 1.0036711422752594E-5 6 1.1470527340288679E-4 3.0110134268257782E-5 1.1470527340288678E-5 4.874974119622688E-5 2.0073422845505188E-5 7 1.1757290523795895E-4 3.441158202086604E-5 1.1470527340288678E-5 5.7352636701443395E-5 2.0073422845505188E-5 8 1.1900672115549504E-4 3.871302977347429E-5 1.2904343257824764E-5 6.308790037158774E-5 2.2941054680577357E-5 9 1.233081689081033E-4 4.3014477526082545E-5 1.4338159175360849E-5 6.59555322066599E-5 2.437487059811344E-5 10-11 1.3406178828962394E-4 4.9466649154994926E-5 1.9356514886737144E-5 7.45584277118764E-5 3.154395018579386E-5 12-13 1.5915356684650543E-4 6.523862424789186E-5 2.652559447441757E-5 8.316132321709293E-5 3.656230589717016E-5 14-15 1.770762658157065E-4 7.814296750571661E-5 3.3694674062097995E-5 9.678257443368573E-5 3.871302977347429E-5 16-17 1.942820568261395E-4 8.602895505216509E-5 3.942993773224233E-5 1.1398836544411874E-4 4.086375364977842E-5 18-19 2.1292166375410862E-4 1.0323474606259812E-4 4.73159252786908E-5 1.2474198482563938E-4 4.3014477526082545E-5 20-21 2.3873035026975814E-4 1.1040382565027853E-4 4.73159252786908E-5 1.4123086787730437E-4 4.73159252786908E-5 22-23 2.717081163730881E-4 1.1613908932042288E-4 5.161737303129905E-5 1.5413521113512912E-4 4.874974119622688E-5 24-25 3.312114769508356E-4 1.2474198482563938E-4 5.5201912825139263E-5 1.7062409418679408E-4 5.0900465072531014E-5 26-27 3.8139503406459856E-4 1.3262797237208787E-4 5.591882078390731E-5 1.8424534540338692E-4 5.4485004866371226E-5 28-29 4.1867424792053676E-4 1.4409849971237651E-4 5.591882078390731E-5 2.1363857171287663E-4 6.165408445405165E-5 30-31 5.964674216950113E-4 1.5270139521759301E-4 5.950336057774753E-5 2.3729653435222204E-4 6.738934812419599E-5 32-33 7.986354660675992E-4 1.6130429072280957E-4 6.452171628912383E-5 2.559361412801911E-4 7.312461179434033E-5 34-35 8.817967892846922E-4 1.756424498981704E-4 7.240770383557229E-5 2.7027430045555197E-4 7.384151975310836E-5 36-37 9.549214010790325E-4 1.8998060907353124E-4 7.384151975310836E-5 2.8389555167214475E-4 7.59922436294125E-5 38-39 0.00104740252776011 2.0360186029012405E-4 7.885987546448466E-5 2.9536607901243345E-4 7.957678342325271E-5 40-41 0.001148486549946404 2.179400194654849E-4 8.316132321709293E-5 3.097042381877943E-4 8.316132321709293E-5 42-43 0.0013133753804630537 2.315612706820777E-4 8.817967892846921E-5 3.2691002919822736E-4 8.602895505216509E-5 44-45 0.0014746796711858634 2.4159798210483032E-4 9.104731076354139E-5 3.412481883735882E-4 8.602895505216509E-5 46-47 0.0016603588325067864 2.616714049503355E-4 9.391494259861357E-5 3.584539793840212E-4 8.817967892846921E-5 48-49 0.0018990891827765444 2.889139073835211E-4 1.0180093014506203E-4 3.7207523060061405E-4 9.391494259861357E-5 50-51 0.0022216977642221638 3.0325206655888194E-4 1.0825310177397442E-4 3.9143174548735113E-4 9.606566647491769E-5 52-53 0.002652559447441757 3.1687331777547473E-4 1.0968691769151049E-4 4.0075154895133575E-4 1.0108402218629399E-4 54-55 0.0032483099611780005 3.2834384511576343E-4 1.1685599727919092E-4 4.0935444445655225E-4 1.0897000973274245E-4 56-57 0.0040075154895133575 3.520018077551088E-4 1.2187435299056722E-4 4.1795733996176875E-4 1.1542218136165483E-4 58-59 0.004928742216530292 3.6849069080677384E-4 1.233081689081033E-4 4.408983946423461E-4 1.2044053707303113E-4 60-61 0.006211290554766319 3.806781261058305E-4 1.2474198482563938E-4 4.616887254466193E-4 1.2259126094933527E-4 62-63 0.008020766242696858 3.871302977347429E-4 1.2545889278440744E-4 4.7746070053951623E-4 1.2760961666071155E-4 64-65 0.010357886188280676 4.000346409925677E-4 1.2689270870194352E-4 4.939495835911813E-4 1.333448803308559E-4 66-67 0.0134957923238084 4.158066160854646E-4 1.3047724849578373E-4 5.104384666428462E-4 1.3621251216592808E-4 68-69 0.017576432425116098 4.287109593432894E-4 1.319110644133198E-4 5.22625901941903E-4 1.3908014400100023E-4 70-71 0.023009160936660323 4.408983946423461E-4 1.3477869624839198E-4 5.326626133646556E-4 1.4696613154744872E-4 72-73 0.030390445280136087 4.530858299414028E-4 1.405139599185363E-4 5.527360362101607E-4 1.5485211909389715E-4 74-75 0.040355465907011875 4.695747129930678E-4 1.5055067134128893E-4 5.685080113030577E-4 1.6130429072280957E-4 76-77 0.05377956743494347 4.925157676736451E-4 1.5413521113512914E-4 5.799785386433464E-4 1.6488883051664975E-4 78-79 0.0718105195059185 5.161737303129906E-4 1.6632264643418585E-4 6.014857774063875E-4 1.6632264643418585E-4 80-81 0.09629149248192961 5.383978770347998E-4 1.670395543929539E-4 6.179746604580525E-4 1.7277481806309822E-4 82-83 0.12910293593882538 5.491514964163205E-4 1.7134100214556214E-4 6.351804514684856E-4 1.7851008173324257E-4 84-85 0.17319134158714244 5.613389317153773E-4 1.7205791010433018E-4 6.473678867675424E-4 1.8424534540338692E-4 86-87 0.23172257497280052 5.735263670144339E-4 1.756424498981704E-4 6.638567698192073E-4 1.8854679315599514E-4 88 0.2861208339761608 5.849968943547226E-4 1.7779317377447453E-4 6.796287449121043E-4 1.9213133294983538E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 47455 0.0 10.399072 94 AGATCGG 56265 0.0 9.7480955 94 ATCGGAA 45710 0.0 9.438842 94 TAGATCG 18385 0.0 9.228494 94 AGCGTCG 17545 0.0 9.000635 94 GCGTCGT 15785 0.0 8.545246 94 TCGGAAG 49345 0.0 7.6101093 94 AAGAGCG 35455 0.0 7.0654035 94 AGAGCGT 28310 0.0 6.8896236 94 AAGATCG 25600 0.0 6.7928033 94 CGTCGTG 15680 0.0 6.77407 94 GAGCGTC 25990 0.0 6.654705 94 CGGAAGA 54660 0.0 6.629366 94 GTCGTGT 16230 0.0 6.3128467 94 CAGATCG 26835 0.0 6.1474185 94 TCGTGTA 14115 0.0 5.9601803 94 GAGATCG 33970 0.0 5.1329336 94 >>END_MODULE