FastQCFastQC Report
Wed 25 May 2016
SRR2049545_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049545_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69743960
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA477950.010.27786694
AGATCGG557400.09.74899994
ATCGGAA458150.09.5318594
TAGATCG191150.09.09906194
TCGGAAG490350.07.82263694
AAGATCG268750.06.909041494
CGGAAGA532650.06.813096594
CAGATCG254400.06.282478394
GAGATCG336350.05.240937794
ACACGTC207650.05.2067394
Produced by FastQC (version 0.11.5)