##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049545_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	69743960
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.831264900931924	33.0	31.0	34.0	30.0	34.0
2	32.15599542956838	34.0	31.0	34.0	30.0	34.0
3	32.254087880871694	34.0	31.0	34.0	30.0	34.0
4	35.78311978556996	37.0	35.0	37.0	33.0	37.0
5	35.84471000786305	37.0	35.0	37.0	35.0	37.0
6	35.87075554643011	37.0	35.0	37.0	35.0	37.0
7	35.87199657145938	37.0	35.0	37.0	35.0	37.0
8	35.87014551510984	37.0	35.0	37.0	35.0	37.0
9	37.58035305136101	39.0	37.0	39.0	35.0	39.0
10-11	37.57127164846963	39.0	37.0	39.0	35.0	39.0
12-13	37.557426069583656	39.0	37.0	39.0	35.0	39.0
14-15	38.89339841041432	40.0	38.0	41.0	35.0	41.0
16-17	38.884336321023355	40.0	38.0	41.0	35.0	41.0
18-19	38.89608543162734	40.0	38.0	41.0	35.0	41.0
20-21	38.898332966754396	40.0	38.0	41.0	35.0	41.0
22-23	38.86402226085241	40.0	38.0	41.0	35.0	41.0
24-25	38.83640821370051	40.0	38.0	41.0	35.0	41.0
26-27	38.72916713504653	40.0	38.0	41.0	34.5	41.0
28-29	38.675484859190675	40.0	38.0	41.0	34.0	41.0
30-31	38.6189668897493	40.0	38.0	41.0	34.0	41.0
32-33	38.555048508860125	40.0	38.0	41.0	34.0	41.0
34-35	38.47639056486038	40.0	38.0	41.0	34.0	41.0
36-37	38.41431327530011	40.0	38.0	41.0	34.0	41.0
38-39	38.34018651220837	40.0	38.0	41.0	34.0	41.0
40-41	38.24720666133669	40.0	38.0	41.0	33.5	41.0
42-43	38.16339982702445	40.0	38.0	41.0	33.0	41.0
44-45	38.076199243633425	40.0	37.0	41.0	33.0	41.0
46-47	37.95541731499043	40.0	37.0	41.0	33.0	41.0
48-49	37.84489169384703	40.0	37.0	41.0	33.0	41.0
50-51	37.71287202504705	40.0	36.5	41.0	33.0	41.0
52-53	37.55748478434548	40.0	36.0	41.0	33.0	41.0
54-55	37.387061560599655	39.0	36.0	41.0	32.0	41.0
56-57	37.21227167198421	39.0	35.0	41.0	32.0	41.0
58-59	37.020120523698395	39.0	35.0	41.0	32.0	41.0
60-61	36.80636832207405	39.0	35.0	41.0	31.5	41.0
62-63	36.558743072518396	38.0	35.0	40.0	31.0	41.0
64-65	36.312958950137045	37.5	35.0	40.0	31.0	41.0
66-67	36.026054162969814	37.0	35.0	40.0	31.0	41.0
68-69	35.73266271373177	36.5	35.0	39.5	31.0	41.0
70-71	35.43271516271803	36.0	34.0	39.0	30.0	41.0
72-73	35.13311060197901	35.5	34.0	39.0	30.0	41.0
74-75	34.81124929671329	35.0	34.0	38.5	30.0	40.0
76-77	34.49364428833694	35.0	34.0	37.0	30.0	39.0
78-79	34.18185078679215	35.0	34.0	37.0	30.0	39.0
80-81	33.9069638575154	35.0	34.0	36.0	30.0	39.0
82-83	33.643664204040036	35.0	34.0	36.0	29.0	37.0
84-85	33.40689896300698	35.0	34.0	35.5	29.0	37.0
86-87	33.19433865670949	35.0	34.0	35.0	29.0	37.0
88-89	33.01060115456593	35.0	33.0	35.0	29.0	36.0
90-91	32.85279173995856	35.0	33.0	35.0	29.0	36.0
92-93	32.69748745267691	35.0	33.0	35.0	29.0	36.0
94-95	32.503385712540556	35.0	33.0	35.0	29.0	35.5
96-97	32.38327450147655	35.0	33.0	35.0	29.0	35.0
98-99	32.25387310241632	35.0	33.0	35.0	29.0	35.0
100	32.14450406888281	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5654732118448571
1101	2	0.4677141000905465
1101	3	0.4159924272722364
1101	4	0.3034645161334524
1101	5	0.25485894449079183
1101	6	0.28790620971239633
1101	7	0.2835692822580782
1101	8	0.3019451636811681
1101	9	0.37021396756553315
1101	10-11	0.35480621068874285
1101	12-13	0.3934748547404894
1101	14-15	0.5212582774918033
1101	16-17	0.5163726179351968
1101	18-19	0.5025200601613236
1101	20-21	0.529541547032828
1101	22-23	0.49950785604399783
1101	24-25	0.5154264892408449
1101	26-27	0.5495610193928755
1101	28-29	0.5516732438705674
1101	30-31	0.532801628136788
1101	32-33	0.5270107371703361
1101	34-35	0.5303794127688022
1101	36-37	0.5523207006423334
1101	38-39	0.5703724191259525
1101	40-41	0.5672635829173771
1101	42-43	0.5747491034344208
1101	44-45	0.5643817558916808
1101	46-47	0.5784909428904044
1101	48-49	0.5809912720336854
1101	50-51	0.5605379181555818
1101	52-53	0.576412013839608
1101	54-55	0.5627699739557315
1101	56-57	0.5547600492501488
1101	58-59	0.5318242744470751
1101	60-61	0.5267722576723486
1101	62-63	0.5484062232543678
1101	64-65	0.5622941787399469
1101	66-67	0.5365358864413565
1101	68-69	0.5433082205668711
1101	70-71	0.5321229126993501
1101	72-73	0.49775479646378784
1101	74-75	0.4889956527949266
1101	76-77	0.48908733972128005
1101	78-79	0.458617922540256
1101	80-81	0.45580336576897906
1101	82-83	0.47002726533820294
1101	84-85	0.4417168498669426
1101	86-87	0.44972538882745994
1101	88-89	0.44251062847931877
1101	90-91	0.4425119975312981
1101	92-93	0.47340604880078274
1101	94-95	0.42289932767466354
1101	96-97	0.4941764305768217
1101	98-99	0.49452943777654923
1101	100	0.4731588428198563
1102	1	0.5533206366895875
1102	2	0.4495789109987385
1102	3	0.4067710519607317
1102	4	0.28617955673837514
1102	5	0.27049168695194936
1102	6	0.2782498085949996
1102	7	0.28486751045119973
1102	8	0.2831187847862324
1102	9	0.35037307793770367
1102	10-11	0.3409872295440266
1102	12-13	0.36404516818625154
1102	14-15	0.4674390573540208
1102	16-17	0.45641147583167196
1102	18-19	0.469007517646169
1102	20-21	0.45450160726591093
1102	22-23	0.4390591642238135
1102	24-25	0.47045106888616317
1102	26-27	0.4748129271794923
1102	28-29	0.4984865375043839
1102	30-31	0.4833050358366364
1102	32-33	0.47184491779916016
1102	34-35	0.4758583542231065
1102	36-37	0.4675169991297494
1102	38-39	0.49588830354471014
1102	40-41	0.48520600104737355
1102	42-43	0.5148893945040882
1102	44-45	0.505867836788596
1102	46-47	0.5331424235549349
1102	48-49	0.5054878732960759
1102	50-51	0.5197861681775535
1102	52-53	0.5093541795026866
1102	54-55	0.5098866251593819
1102	56-57	0.5136854273079265
1102	58-59	0.5075477612380226
1102	60-61	0.4913603513372493
1102	62-63	0.5076399590195209
1102	64-65	0.5188998896331469
1102	66-67	0.47913357004486556
1102	68-69	0.50090388736146
1102	70-71	0.513379278389678
1102	72-73	0.47422427618220553
1102	74-75	0.4600383429453814
1102	76-77	0.47491897136701766
1102	78-79	0.4546070838506324
1102	80-81	0.42230476264693095
1102	82-83	0.4467160951578677
1102	84-85	0.43118046746318583
1102	86-87	0.40714985476084564
1102	88-89	0.43606973855153086
1102	90-91	0.44568731197546185
1102	92-93	0.46072730051385236
1102	94-95	0.4325567881044847
1102	96-97	0.4564342919980007
1102	98-99	0.467953471583634
1102	100	0.449599754756953
1103	1	0.5023949680592743
1103	2	0.3950369143417376
1103	3	0.3539608113351562
1103	4	0.2523093809527168
1103	5	0.24892240324898296
1103	6	0.23279543256522572
1103	7	0.25524437410962975
1103	8	0.23642553567378144
1103	9	0.29636009367037275
1103	10-11	0.30252407111530033
1103	12-13	0.3063824324261759
1103	14-15	0.4128162939453901
1103	16-17	0.4079349489656465
1103	18-19	0.40765061931873703
1103	20-21	0.4008600935459228
1103	22-23	0.3920389245506115
1103	24-25	0.38848741209805837
1103	26-27	0.42581771350554476
1103	28-29	0.43211862949413415
1103	30-31	0.4166204562322946
1103	32-33	0.42746012432058933
1103	34-35	0.4435733076416639
1103	36-37	0.4358053255078076
1103	38-39	0.458604123209426
1103	40-41	0.4635991268312267
1103	42-43	0.47054303038975576
1103	44-45	0.45917765765357643
1103	46-47	0.47208651238343435
1103	48-49	0.4538275660279183
1103	50-51	0.4604992597385049
1103	52-53	0.4465074809537981
1103	54-55	0.45723034979778987
1103	56-57	0.4455337868860241
1103	58-59	0.42531114480159715
1103	60-61	0.438542695252238
1103	62-63	0.48306050118875277
1103	64-65	0.46712367406438204
1103	66-67	0.457850301821324
1103	68-69	0.46593065355816776
1103	70-71	0.4549746180730594
1103	72-73	0.4196483210996149
1103	74-75	0.4350650351968852
1103	76-77	0.38892285994180753
1103	78-79	0.3983047717437529
1103	80-81	0.374067103493843
1103	82-83	0.39575130016282856
1103	84-85	0.36289584375287376
1103	86-87	0.3946326335579613
1103	88-89	0.37796793395271067
1103	90-91	0.4058691761266715
1103	92-93	0.4026834997935538
1103	94-95	0.34510570082757397
1103	96-97	0.412103460980056
1103	98-99	0.40834523395123057
1103	100	0.42216031458439573
1104	1	0.4457179801215183
1104	2	0.3078942661245634
1104	3	0.288167624056193
1104	4	0.2242321151085065
1104	5	0.1739132169163895
1104	6	0.17475972597739542
1104	7	0.16988122525621918
1104	8	0.17053715950114423
1104	9	0.22602515856384287
1104	10-11	0.26043516710713277
1104	12-13	0.26181353463479695
1104	14-15	0.3336841945280682
1104	16-17	0.34393106703385
1104	18-19	0.35761701390959644
1104	20-21	0.3447685846459265
1104	22-23	0.337782879920006
1104	24-25	0.3233397945295451
1104	26-27	0.3589917021731779
1104	28-29	0.36660510197797436
1104	30-31	0.37093071631974794
1104	32-33	0.3480191340329952
1104	34-35	0.3649175926650159
1104	36-37	0.34334053202120174
1104	38-39	0.3535411765148453
1104	40-41	0.37389909129841925
1104	42-43	0.38344957843194294
1104	44-45	0.3865409757454401
1104	46-47	0.3961839756757968
1104	48-49	0.381586103098563
1104	50-51	0.4033660593114732
1104	52-53	0.37108428304318153
1104	54-55	0.36612421405791196
1104	56-57	0.3505243391257977
1104	58-59	0.38216085714638837
1104	60-61	0.37442896210797727
1104	62-63	0.3842884406033349
1104	64-65	0.3682684850259079
1104	66-67	0.3610770372016603
1104	68-69	0.35482468135467116
1104	70-71	0.3561905851937581
1104	72-73	0.300307589518205
1104	74-75	0.3353413092770978
1104	76-77	0.3121968606516248
1104	78-79	0.29642088452443716
1104	80-81	0.26199616667592807
1104	82-83	0.2986775423206893
1104	84-85	0.2705932292579476
1104	86-87	0.2982101492970486
1104	88-89	0.30733921173527534
1104	90-91	0.3144537598438433
1104	92-93	0.2905146422403817
1104	94-95	0.24489984129945697
1104	96-97	0.2975909760547708
1104	98-99	0.30761352520453755
1104	100	0.2907433199694509
1105	1	0.39024311350270224
1105	2	0.30032847688440256
1105	3	0.2359612209307258
1105	4	0.18599977721376604
1105	5	0.15960735140197357
1105	6	0.1774496293503276
1105	7	0.17583512673335377
1105	8	0.16260628370439179
1105	9	0.21276392191415283
1105	10-11	0.19906337361379656
1105	12-13	0.21356556768703427
1105	14-15	0.3111301651381311
1105	16-17	0.2995554404447631
1105	18-19	0.3118083554098732
1105	20-21	0.28632134388904973
1105	22-23	0.28351433672155935
1105	24-25	0.2836278256149072
1105	26-27	0.344448777044569
1105	28-29	0.3270259690452164
1105	30-31	0.3267344782513888
1105	32-33	0.31401217071586984
1105	34-35	0.3241784041620406
1105	36-37	0.3072004311225598
1105	38-39	0.2774351520990095
1105	40-41	0.3090949859007637
1105	42-43	0.343318818199279
1105	44-45	0.35562818505298566
1105	46-47	0.3616125765974303
1105	48-49	0.36419591485783087
1105	50-51	0.3471944159278735
1105	52-53	0.3457155301903114
1105	54-55	0.3480089820569674
1105	56-57	0.3543660828330317
1105	58-59	0.3462940054019157
1105	60-61	0.35531790217130776
1105	62-63	0.3572382716666951
1105	64-65	0.3401419834676318
1105	66-67	0.3263011537830991
1105	68-69	0.32557724542979827
1105	70-71	0.32675153694674464
1105	72-73	0.2923655031102683
1105	74-75	0.3017293381499613
1105	76-77	0.2923353678759639
1105	78-79	0.265626267984743
1105	80-81	0.2637441557701905
1105	82-83	0.2507172529362691
1105	84-85	0.25385684718865065
1105	86-87	0.2594140736808157
1105	88-89	0.27403888902143336
1105	90-91	0.2814618220009777
1105	92-93	0.30860847168197836
1105	94-95	0.25597143384402443
1105	96-97	0.32514248214203434
1105	98-99	0.31084644133382255
1105	100	0.2776986698723434
1106	1	0.28743715467973985
1106	2	0.20251261032341716
1106	3	0.15539032516598894
1106	4	0.10256363737507002
1106	5	0.11496282987162942
1106	6	0.11855980477853478
1106	7	0.11618188977928412
1106	8	0.143530953874631
1106	9	0.12165914044330606
1106	10-11	0.15768963615138887
1106	12-13	0.15516376053537329
1106	14-15	0.1913068970659353
1106	16-17	0.20379273902324258
1106	18-19	0.2087151804127032
1106	20-21	0.20784082631070788
1106	22-23	0.20632159274673256
1106	24-25	0.21520030278082913
1106	26-27	0.21596280040765237
1106	28-29	0.23782596473377993
1106	30-31	0.23659024794893924
1106	32-33	0.21716370762459292
1106	34-35	0.2283781870585173
1106	36-37	0.23517828994071266
1106	38-39	0.24576271585336684
1106	40-41	0.2459460024057165
1106	42-43	0.2293614775761128
1106	44-45	0.25771697044341835
1106	46-47	0.23477126987676655
1106	48-49	0.27742952205338867
1106	50-51	0.23403729451531063
1106	52-53	0.26205130296120416
1106	54-55	0.20936923895229143
1106	56-57	0.23647005921726816
1106	58-59	0.2537714971170857
1106	60-61	0.22742785456380687
1106	62-63	0.22694340316629535
1106	64-65	0.22203139498135727
1106	66-67	0.2356525223038446
1106	68-69	0.24633650531067985
1106	70-71	0.21716838032956787
1106	72-73	0.17301172104017581
1106	74-75	0.19856516726734696
1106	76-77	0.20296564608315748
1106	78-79	0.18360624631667122
1106	80-81	0.16692930243554827
1106	82-83	0.1690416965245305
1106	84-85	0.13049041374735282
1106	86-87	0.220619472107785
1106	88-89	0.20499461509786698
1106	90-91	0.22457785381647
1106	92-93	0.19623955836480889
1106	94-95	0.15843126724112722
1106	96-97	0.23208331437894714
1106	98-99	0.20674706227701023
1106	100	0.194046001502727
1107	1	0.285791986163769
1107	2	0.20941772334923314
1107	3	0.15930795088738847
1107	4	0.1616245682379187
1107	5	0.12929548229438836
1107	6	0.09862574541360658
1107	7	0.09110136883482767
1107	8	0.09119723505254029
1107	9	0.10492194506563379
1107	10-11	0.12614222754440618
1107	12-13	0.13871011728816995
1107	14-15	0.19897558850176722
1107	16-17	0.1721911785520973
1107	18-19	0.16834062452334564
1107	20-21	0.14031453553412376
1107	22-23	0.1713066135049175
1107	24-25	0.18764751032304616
1107	26-27	0.19960390311731402
1107	28-29	0.19034191447580895
1107	30-31	0.20202942778454513
1107	32-33	0.21083528792429718
1107	34-35	0.21269935488751202
1107	36-37	0.18618109336949118
1107	38-39	0.20312550251596662
1107	40-41	0.22433895361290723
1107	42-43	0.2196339266757903
1107	44-45	0.22299905676199927
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2216	84-85	-0.6951914303527218
2216	86-87	-0.6621965773320397
2216	88-89	-0.6934822175925817
2216	90-91	-0.7202193506485344
2216	92-93	-0.7859894515488648
2216	94-95	-0.751825982221316
2216	96-97	-0.7893291706877577
2216	98-99	-0.7929562333624958
2216	100	-0.8284761735244537
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	13485.0
3	2127.0
4	1873.0
5	2538.0
6	4433.0
7	8672.0
8	16505.0
9	25217.0
10	32892.0
11	40500.0
12	46267.0
13	51732.0
14	57010.0
15	63081.0
16	69757.0
17	76715.0
18	84021.0
19	93500.0
20	105435.0
21	120377.0
22	140243.0
23	170801.0
24	215135.0
25	281361.0
26	375883.0
27	502283.0
28	667254.0
29	877404.0
30	1138482.0
31	1467613.0
32	1891128.0
33	2483298.0
34	3432372.0
35	5167938.0
36	8828733.0
37	1.5356491E7
38	1.9534954E7
39	6278821.0
40	17629.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.629460242577643	27.271626157617924	25.334733477222382	23.76418012258205
2	23.576286749418873	26.562863651562086	25.90924719502592	23.951602403993117
3	23.37162673298161	25.66306960488048	26.506080239779905	24.459223422358008
4	23.8440977541281	25.61899697120726	26.468273381666314	24.068631892998333
5	23.940475174682703	25.845352918479094	26.341510702731018	23.87266120410719
6	23.853642114823554	25.681913423175693	26.026246238360677	24.43819822364007
7	23.707870903803	25.910255167615947	26.414070838535697	23.96780309004536
8	23.405330009939213	26.045227429013206	26.46733566605624	24.082106894991337
9	23.331620401250515	25.837427355716535	26.67093896016228	24.16001328287066
10-11	23.34622955163429	26.103022397925212	26.678223461931328	23.87252458850917
12-13	23.274959084983358	25.945311437440616	26.768319808435688	24.011409669140342
14-15	23.350611894104095	25.909747969315543	26.73937812064258	24.00026201593778
16-17	23.423802577312788	25.845942071542826	26.71821904004304	24.012036311101348
18-19	23.445904849681607	25.7130000934848	26.6624450346668	24.178650022166796
20-21	23.507961836408487	25.68947762644966	26.614867437983158	24.187693099158693
22-23	23.595904218802602	25.75442733679017	26.518991752117316	24.130676692289914
24-25	23.644739988953884	25.716794687310557	26.45450803195001	24.183957291785553
26-27	23.721236218878307	25.704107567164243	26.417808079724754	24.1568481342327
28-29	23.748402729067863	25.765531524163528	26.3759858201341	24.110079926634505
30-31	23.79788879261097	25.707475083087438	26.3361677736222	24.158468350679392
32-33	23.849770646805833	25.717378967296955	26.27272598229295	24.160124403604268
34-35	23.871403344461655	25.7426643110027	26.26029909973566	24.125633244799978
36-37	23.892310098514375	25.693282455282713	26.228580665788257	24.185826780414654
38-39	23.912384544363064	25.663983612538548	26.236176006253555	24.187455836844837
40-41	23.946776203251112	25.7141397688347	26.200477328680012	24.13860669923418
42-43	24.00440849754448	25.67167022573247	26.13199204874881	24.191929227974242
44-45	24.01473525145885	25.654434054392983	26.132246406358096	24.198584287790073
46-47	24.050295287294134	25.696945132237893	26.091137320539247	24.161622259928723
48-49	24.06944096175406	25.64465772907529	26.05034936865906	24.235551940511588
50-51	24.065857353387052	25.662973498782073	26.052779103982065	24.21839004384881
52-53	24.09617794736642	25.708267692767112	26.027641041309877	24.167913318556586
54-55	24.12537284628087	25.662389207140585	25.996194075208596	24.21604387136995
56-57	24.12267640410865	25.66358771265881	25.997537417015575	24.216198466216962
58-59	24.147364674397963	25.703073977188538	25.98221706899904	24.167344279414458
60-61	24.150974261145457	25.676648432660926	25.949636968176215	24.2227403380174
62-63	24.162111557355406	25.674100366762353	25.95459142107058	24.209196654811656
64-65	24.194678106854546	25.732972616789844	25.91471882235192	24.15763045400369
66-67	24.20219065988996	25.697508704175604	25.888993039885612	24.211307596048826
68-69	24.19246649361741	25.694422175047304	25.896187752357076	24.216923578978207
70-71	24.245668180129584	25.734692614315207	25.860647641839275	24.158991563715933
72-73	24.25300626542025	25.74301059196195	25.821079757125798	24.182903385491997
74-75	24.272147724462627	25.740635401928113	25.825994611005854	24.161222262603406
76-77	24.277672260897802	25.787209705240667	25.808988177059966	24.126129856801565
78-79	24.29217965532731	25.765816577880194	25.792395630743243	24.14960813604925
80-81	24.291917629979825	25.77787786633753	25.793404226842124	24.13680027684052
82-83	24.299610484876503	25.831357989146458	25.78247718197867	24.086554343998376
84-85	24.30293657918925	25.82338772112058	25.7445650627105	24.129110636979675
86-87	24.30164890196278	25.844074484857043	25.74515972460706	24.109116888573116
88-89	24.33681229150602	25.89189255690714	25.706107641629362	24.065187509957475
90-91	24.341575085003424	25.877991710819988	25.68045733606712	24.09997586810947
92-93	24.34020529855626	25.92004234343414	25.67019990242484	24.06955245558476
94-95	24.38509591263311	25.979247201922185	25.627080249754115	24.00857663569059
96-97	24.378440659064893	26.009459771916948	25.575082636324954	24.03701693269321
98-99	24.3880167747242	26.067547510771373	25.54857726731889	23.99585844718554
100	24.431935529476675	26.13503061128217	25.48518789038382	23.947845968857333
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	41.0
1	40.0
2	40.0
3	42.5
4	45.5
5	59.5
6	90.5
7	114.0
8	139.0
9	193.5
10	262.5
11	354.0
12	517.5
13	790.0
14	1263.5
15	2181.0
16	3743.5
17	6368.5
18	11045.5
19	18673.5
20	30352.5
21	46997.5
22	69978.0
23	101921.5
24	143334.5
25	194723.0
26	257940.0
27	332499.5
28	419048.0
29	518054.5
30	626078.5
31	744561.5
32	877218.5
33	1023249.0
34	1181184.0
35	1351486.0
36	1538170.5
37	1746983.5
38	1970932.5
39	2189736.5
40	2382443.5
41	2527510.5
42	2606354.0
43	2621259.5
44	2582668.5
45	2495566.0
46	2365788.0
47	2219097.5
48	2078260.5
49	1952209.0
50	1849828.0
51	1769673.5
52	1710113.0
53	1678050.5
54	1675610.5
55	1695203.5
56	1722449.0
57	1732400.0
58	1712878.5
59	1666748.0
60	1601437.5
61	1519972.0
62	1420794.5
63	1305932.0
64	1178095.5
65	1038427.5
66	899227.5
67	774439.0
68	657737.5
69	548310.5
70	454161.5
71	373032.0
72	302719.0
73	243562.0
74	194757.0
75	156648.0
76	127615.5
77	105472.0
78	86423.0
79	68671.5
80	54656.5
81	43841.5
82	35166.0
83	27530.5
84	20853.0
85	15337.5
86	11245.0
87	8039.0
88	5499.0
89	3576.0
90	2464.5
91	1688.5
92	859.0
93	419.0
94	217.5
95	133.0
96	79.0
97	49.5
98	43.0
99	81.0
100	130.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1606863160623515
2	0.0
3	0.0
4	0.0
5	3.140056859404026E-4
6	1.419477758360724E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0022001905254591223
14-15	1.5915356684650543E-4
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	3.828288499821347E-4
32-33	0.0
34-35	0.0
36-37	0.0017865346332499618
38-39	0.0026647468827408136
40-41	0.002380134423109901
42-43	6.75327297159496E-4
44-45	7.670915158818054E-5
46-47	0.00172488054879591
48-49	3.2332548940438715E-4
50-51	3.1830713369301086E-4
52-53	0.0011764459603383576
54-55	1.0036711422752594E-4
56-57	7.506026328301405E-4
58-59	4.860635960447328E-4
60-61	7.885987546448467E-6
62-63	3.5128489979634076E-5
64-65	7.169079587680425E-7
66-67	1.9356514886737145E-4
68-69	0.0019922872174163897
70-71	0.001165692340956837
72-73	5.018355711376297E-5
74-75	0.0023392706694601224
76-77	0.0011104904281316978
78-79	0.0015320323078873068
80-81	0.0018345674664874204
82-83	0.0012044053707303112
84-85	7.885987546448466E-5
86-87	0.005176075462305267
88-89	0.00521478849207874
90-91	2.1435547967164467E-4
92-93	0.03788356726517967
94-95	0.010454668762714362
96-97	5.993350535300835E-4
98-99	0.0035637494630359388
100	0.002437487059811344
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6.974396E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.90509559506418
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.0457659867169	54.712187432664116
2	17.3336413583994	25.274215604893335
3	5.0549846291007565	11.056024128485122
4	1.6373639216250717	4.774886929199402
5	0.5591925332882327	2.0383992547712353
6	0.20840322532177064	0.9116194238642035
7	0.07669546398318974	0.3914043093381575
8	0.0328895344988953	0.19182517253752987
9	0.014857807630732143	0.0974888897086441
>10	0.03548629676603336	0.5132772066267829
>50	6.731698308501126E-4	0.02920672828426152
>100	4.409314724524632E-5	0.004411941720573668
>500	0.0	0.0
>1k	1.9796909822866266E-6	0.00505297790653338
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.176421872230943E-5	2.8676318350721696E-6	2.8676318350721696E-6	1.0036711422752594E-5	1.4338159175360848E-6
2	9.749948239245376E-5	2.8676318350721696E-6	5.735263670144339E-6	2.1507238763041272E-5	1.4338159175360848E-6
3	9.893329830998985E-5	5.735263670144339E-6	7.169079587680424E-6	2.7242502433185613E-5	1.4338159175360848E-6
4	1.0466856198013419E-4	7.169079587680424E-6	7.169079587680424E-6	4.1580661608546464E-5	2.8676318350721696E-6
5	1.1183764156781461E-4	1.4338159175360849E-5	8.60289550521651E-6	5.018355711376297E-5	2.8676318350721696E-6
6	1.132714574853507E-4	2.1507238763041272E-5	8.60289550521651E-6	5.305118894883514E-5	2.8676318350721696E-6
7	1.1757290523795895E-4	2.437487059811344E-5	1.0036711422752594E-5	6.308790037158774E-5	2.8676318350721696E-6
8	1.2187435299056722E-4	2.7242502433185613E-5	1.0036711422752594E-5	7.312461179434033E-5	2.8676318350721696E-6
9	1.2760961666071155E-4	3.297776610332995E-5	1.1470527340288678E-5	9.319803463984551E-5	4.301447752608255E-6
10-11	1.3549560420716002E-4	4.444829344361863E-5	1.3621251216592807E-5	1.032347460625981E-4	5.018355711376297E-6
12-13	1.5485211909389715E-4	5.663572874267535E-5	1.8639606927969104E-5	1.2617580074317545E-4	6.452171628912381E-6
14-15	1.6703955439295386E-4	7.59922436294125E-5	2.4374870598113444E-5	1.4911685542375283E-4	7.169079587680424E-6
16-17	1.770762658157065E-4	9.534875851614964E-5	3.154395018579387E-5	1.706240941867941E-4	8.60289550521651E-6
18-19	1.9643278070244363E-4	1.1183764156781463E-4	3.441158202086604E-5	1.8998060907353124E-4	1.1470527340288678E-5
20-21	2.143554796716447E-4	1.319110644133198E-4	3.5845397938402126E-5	2.1578929558918077E-4	1.5055067134128891E-5
22-23	2.4518252189867053E-4	1.4266468379484044E-4	3.727921385593821E-5	2.4088107414606226E-4	1.6488883051664975E-5
24-25	3.132887779816345E-4	1.5556902705266522E-4	3.942993773224233E-5	2.5450232536265504E-4	2.0790330804273232E-5
26-27	3.6633996693046964E-4	1.6345501459911368E-4	4.1580661608546464E-5	2.724250243318561E-4	2.5808686515649525E-5
28-29	4.036191807864079E-4	1.7420863398063432E-4	4.373138548485059E-5	2.889139073835211E-4	2.7959410391953657E-5
30-31	5.828461704784185E-4	1.8137771356831474E-4	5.161737303129905E-5	3.0898733022902624E-4	3.0827042227025826E-5
32-33	7.742605954694858E-4	1.9356514886737145E-4	5.5918820783907314E-5	3.2834384511576343E-4	3.5845397938402126E-5
34-35	8.423668515524499E-4	2.1077093987780448E-4	5.8786452618979476E-5	3.4913417592003664E-4	3.727921385593821E-5
36-37	9.083223837591098E-4	2.229583751768612E-4	6.0220268536515564E-5	3.7207523060061405E-4	4.301447752608255E-5
38-39	9.914837069762027E-4	2.3729653435222204E-4	6.093717649528361E-5	3.907148375285831E-4	4.659901731992276E-5
40-41	0.0010904170052861925	2.5235160148635096E-4	6.738934812419599E-5	4.079206285390162E-4	4.73159252786908E-5
42-43	0.0012481367562151618	2.738588402493922E-4	7.097388791803619E-5	4.301447752608254E-4	4.874974119622688E-5
44-45	0.0014065734151028993	2.8317864371337674E-4	7.312461179434033E-5	4.5165201402386674E-4	5.0900465072531014E-5
46-47	0.0015829327729598376	3.0181825064134587E-4	8.101059934078879E-5	4.745930687044441E-4	5.305118894883514E-5
48-49	0.001825964570982204	3.2189167348685107E-4	8.316132321709293E-5	4.90365043797341E-4	5.663572874267535E-5
50-51	0.0021414040728401427	3.362298326622119E-4	8.602895505216509E-5	5.183244541892947E-4	5.8786452618979476E-5
52-53	0.002556493780966839	3.4770036000250057E-4	9.033040280477335E-5	5.355302451997277E-4	6.237099241281969E-5
54-55	0.0031529612026618505	3.591708873427892E-4	9.176421872230943E-5	5.520191282513926E-4	6.738934812419599E-5
56-57	0.0039035638354919908	3.7637667835322227E-4	9.176421872230943E-5	5.835630784371865E-4	6.810625608296404E-5
58-59	0.004816904574962477	4.036191807864079E-4	9.46318505573816E-5	6.065041331177639E-4	7.312461179434033E-5
60-61	0.006100886729116042	4.158066160854646E-4	9.749948239245378E-5	6.26577555963269E-4	7.59922436294125E-5
62-63	0.007913230048881652	4.322954991371296E-4	1.0251783810383006E-4	6.452171628912382E-4	7.59922436294125E-5
64-65	0.010236011835290111	4.459167503537224E-4	1.0897000973274245E-4	6.789118369533362E-4	7.670915158818054E-5
66-67	0.013383237774281815	4.6885780503429973E-4	1.1183764156781461E-4	7.004190757163775E-4	8.172750729955684E-5
68-69	0.017501157089445454	4.961003074674854E-4	1.132714574853507E-4	7.183417746855784E-4	8.4595139134629E-5
70-71	0.022973315538721922	5.211920860243668E-4	1.1757290523795895E-4	7.319630259021713E-4	8.602895505216509E-5
72-73	0.03042485686215695	5.376809690760319E-4	1.1900672115549504E-4	7.506026328301405E-4	8.817967892846921E-5
74-75	0.04046013446899201	5.613389317153773E-4	1.2187435299056722E-4	7.713929636344137E-4	8.96134948460053E-5
76-77	0.05393872100178998	5.778278147670422E-4	1.2402507686687134E-4	8.000692819851354E-4	9.391494259861357E-5
78-79	0.07207935999045653	6.007688694476196E-4	1.3047724849578373E-4	8.230103366657128E-4	9.749948239245376E-5
80-81	0.09665998317273639	6.208422922931247E-4	1.3621251216592805E-4	8.43800667469986E-4	1.0108402218629399E-4
82-83	0.12958183045528243	6.409157151386299E-4	1.3908014400100023E-4	8.638740903154911E-4	1.046685619801342E-4
84-85	0.1738315403943223	6.638567698192073E-4	1.4266468379484044E-4	8.846644211197643E-4	1.0753619381520636E-4
86-87	0.23260939011779658	6.746103892007279E-4	1.491168554237528E-4	9.12623831511718E-4	1.0968691769151049E-4
88	0.2872320413122513	6.954007200050011E-4	1.505506713412889E-4	9.276788986458469E-4	1.132714574853507E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	47795	0.0	10.277866	94
AGATCGG	55740	0.0	9.748999	94
ATCGGAA	45815	0.0	9.53185	94
TAGATCG	19115	0.0	9.099061	94
TCGGAAG	49035	0.0	7.822636	94
AAGATCG	26875	0.0	6.9090414	94
CGGAAGA	53265	0.0	6.8130965	94
CAGATCG	25440	0.0	6.2824783	94
GAGATCG	33635	0.0	5.2409377	94
ACACGTC	20765	0.0	5.20673	94
>>END_MODULE