FastQCFastQC Report
Wed 25 May 2016
SRR2049544_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049544_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71022171
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC79300.013.3922233
TTCCGAT107750.010.0746632
CCGATCT107200.010.0385154
GATCGGA167800.09.2171694
AGATCGG211450.08.38159894
CGATCTA46750.08.1411875
TAGATCG72300.07.737521694
ATCGGAA178400.07.66816394
CTTCCGA182500.06.10485461
Produced by FastQC (version 0.11.5)