##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049544_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 71022171 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.633769193003126 33.0 31.0 34.0 30.0 34.0 2 31.840749785021355 34.0 31.0 34.0 30.0 34.0 3 31.896468287346497 34.0 31.0 34.0 30.0 34.0 4 35.38892484714386 37.0 35.0 37.0 33.0 37.0 5 35.37387306845351 37.0 35.0 37.0 33.0 37.0 6 35.367698559932784 37.0 35.0 37.0 33.0 37.0 7 35.35477908722334 37.0 35.0 37.0 33.0 37.0 8 35.354289395067916 37.0 35.0 37.0 33.0 37.0 9 37.009303967911656 39.0 37.0 39.0 34.0 39.0 10-11 36.99573031919849 39.0 37.0 39.0 34.0 39.0 12-13 36.97829701657529 39.0 37.0 39.0 34.0 39.0 14-15 38.30540808165382 40.0 38.0 41.0 34.0 41.0 16-17 38.247432115247506 40.0 38.0 41.0 34.0 41.0 18-19 38.28768727725881 40.0 38.0 41.0 34.0 41.0 20-21 38.2772637223382 40.0 38.0 41.0 34.0 41.0 22-23 38.21088849142615 40.0 38.0 41.0 34.0 41.0 24-25 38.173067055356555 40.0 38.0 41.0 34.0 41.0 26-27 38.10449626356817 40.0 38.0 41.0 34.0 41.0 28-29 38.02122423855503 40.0 38.0 41.0 33.0 41.0 30-31 37.95899381898647 40.0 38.0 41.0 33.0 41.0 32-33 37.900030231404784 40.0 38.0 41.0 33.0 41.0 34-35 37.81056952623991 40.0 38.0 41.0 33.0 41.0 36-37 37.72501789617217 40.0 38.0 41.0 33.0 41.0 38-39 37.63038310952224 40.0 38.0 41.0 33.0 41.0 40-41 37.56255223456912 40.0 38.0 41.0 33.0 41.0 42-43 37.429361192014255 40.0 37.5 41.0 32.0 41.0 44-45 37.321140056391684 40.0 37.0 41.0 32.0 41.0 46-47 37.192196377663535 40.0 37.0 41.0 32.0 41.0 48-49 37.07146424741086 40.0 37.0 41.0 31.5 41.0 50-51 36.93373771269256 40.0 36.0 41.0 31.0 41.0 52-53 36.772693628303756 40.0 36.0 41.0 31.0 41.0 54-55 36.60546785453799 39.5 35.0 41.0 31.0 41.0 56-57 36.41207462526033 39.0 35.0 41.0 31.0 41.0 58-59 36.215989792821176 39.0 35.0 41.0 30.0 41.0 60-61 35.96519077824304 39.0 35.0 41.0 30.0 41.0 62-63 35.7320925602232 38.0 35.0 40.5 30.0 41.0 64-65 35.46687223909278 37.5 35.0 40.0 29.0 41.0 66-67 35.19868690017938 37.0 35.0 40.0 29.0 41.0 68-69 34.90170536324495 36.5 34.5 40.0 29.0 41.0 70-71 34.571767758268045 36.0 34.0 39.0 29.0 41.0 72-73 34.27054012471683 35.5 34.0 39.0 29.0 41.0 74-75 33.95990335891028 35.0 34.0 38.5 28.5 40.0 76-77 33.62441739495685 35.0 34.0 37.0 28.0 39.5 78-79 33.33896046348681 35.0 34.0 37.0 28.0 39.0 80-81 33.05162420337729 35.0 34.0 36.5 27.5 39.0 82-83 32.79303858509197 35.0 34.0 36.0 27.0 37.5 84-85 32.54265838339411 35.0 33.5 36.0 27.0 37.0 86-87 32.310397220890366 35.0 33.0 35.0 27.0 37.0 88-89 32.156652645833645 35.0 33.0 35.0 26.5 36.0 90-91 31.9521273645662 35.0 33.0 35.0 26.0 36.0 92-93 31.79889127861214 35.0 33.0 35.0 26.0 36.0 94-95 31.643519254008723 35.0 33.0 35.0 25.5 36.0 96-97 31.51643865547281 35.0 33.0 35.0 25.0 35.0 98-99 31.348058643546675 35.0 33.0 35.0 25.0 35.0 100 31.23748722071591 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26149794644903324 1101 2 0.2924405400447725 1101 3 0.31387065168387096 1101 4 0.3170267343384694 1101 5 0.2945849188690204 1101 6 0.22530452666670442 1101 7 0.20813909003671682 1101 8 0.2266072706185298 1101 9 0.31928805858900233 1101 10-11 0.31680651720677844 1101 12-13 0.3068556074635538 1101 14-15 0.3814852277282412 1101 16-17 0.42798179201552244 1101 18-19 0.42021046261749717 1101 20-21 0.39906433990861956 1101 22-23 0.38986079237206184 1101 24-25 0.4268234585014028 1101 26-27 0.40033626067667427 1101 28-29 0.41262459393170303 1101 30-31 0.41244959876826925 1101 32-33 0.38799999939034535 1101 34-35 0.3800839392105999 1101 36-37 0.408714627912822 1101 38-39 0.45445136927372687 1101 40-41 0.44729293305435647 1101 42-43 0.41463124525392914 1101 44-45 0.4176370586180269 1101 46-47 0.40101977133597444 1101 48-49 0.3911780564250762 1101 50-51 0.4052108345286811 1101 52-53 0.414674684140941 1101 54-55 0.41136441074858965 1101 56-57 0.4499159972655491 1101 58-59 0.41939674007339534 1101 60-61 0.39450133036924484 1101 62-63 0.40222214301249437 1101 64-65 0.42258229538994385 1101 66-67 0.4247204586650426 1101 68-69 0.4204488595795013 1101 70-71 0.39803317213322487 1101 72-73 0.4440773530837845 1101 74-75 0.4234341273248887 1101 76-77 0.41429968863670297 1101 78-79 0.3968727078642331 1101 80-81 0.36679054012628853 1101 82-83 0.39931116811253276 1101 84-85 0.40958471239012084 1101 86-87 0.4403062138840639 1101 88-89 0.39689040302094725 1101 90-91 0.39632731816145395 1101 92-93 0.4094395571270617 1101 94-95 0.38685942272222107 1101 96-97 0.4145789075158497 1101 98-99 0.4200034186643862 1101 100 0.46122645774904214 1102 1 0.2713438922455289 1102 2 0.30202409815066744 1102 3 0.24669984948511114 1102 4 0.2558149891830652 1102 5 0.2302527840853088 1102 6 0.18425473686460236 1102 7 0.19063452836493155 1102 8 0.1630025645320643 1102 9 0.19716775944008447 1102 10-11 0.21284074647176254 1102 12-13 0.1934907227935483 1102 14-15 0.28611809783590303 1102 16-17 0.32900705155484644 1102 18-19 0.29909501273596106 1102 20-21 0.2849592410023547 1102 22-23 0.25270998877819295 1102 24-25 0.24175902602598853 1102 26-27 0.18028506687899437 1102 28-29 0.277023661714793 1102 30-31 0.29894446159975985 1102 32-33 0.19562176374387263 1102 34-35 0.27834095826762706 1102 36-37 0.2682184693936236 1102 38-39 0.30869251654927155 1102 40-41 0.2902972642936774 1102 42-43 0.2799302861388071 1102 44-45 0.22884869330205504 1102 46-47 0.24362179853943644 1102 48-49 0.23446344334049485 1102 50-51 0.2757705246767088 1102 52-53 0.2288041549823916 1102 54-55 0.24124264392983719 1102 56-57 0.3032492293329554 1102 58-59 0.2768230889732095 1102 60-61 0.2562640904049118 1102 62-63 0.27459138192624266 1102 64-65 0.24550905563331327 1102 66-67 0.25020309413027775 1102 68-69 0.2843626627049929 1102 70-71 0.32899862596402585 1102 72-73 0.3141611910419755 1102 74-75 0.30753172369670523 1102 76-77 0.2926325143619408 1102 78-79 0.2614270245253607 1102 80-81 0.252899862078209 1102 82-83 0.2548683860535448 1102 84-85 0.23566042292557654 1102 86-87 0.21934613727493968 1102 88-89 0.18511289748440163 1102 90-91 0.22747942400126675 1102 92-93 0.2423005303592909 1102 94-95 0.2758868873727316 1102 96-97 0.22873704946302809 1102 98-99 0.3150912702541788 1102 100 0.34602918767423674 1103 1 0.1912925320675889 1103 2 0.20869420291296592 1103 3 0.1764771837479273 1103 4 0.13821591359951668 1103 5 0.12143432613217442 1103 6 0.08173838592924199 1103 7 0.05526344796894023 1103 8 0.022877646688414188 1103 9 0.11775571381298278 1103 10-11 0.09038107158072961 1103 12-13 0.022851771982324465 1103 14-15 0.08933375589380432 1103 16-17 0.19337396752779057 1103 18-19 0.1167835743710981 1103 20-21 0.11276665279342524 1103 22-23 0.10089114136183497 1103 24-25 0.08077081724881907 1103 26-27 0.03987057662109805 1103 28-29 0.08192384198906666 1103 30-31 0.10657793878566935 1103 32-33 0.10947767678299414 1103 34-35 0.09934848636468985 1103 36-37 0.07396179913865097 1103 38-39 0.09204225033728619 1103 40-41 0.1286757235739202 1103 42-43 0.14118353614490786 1103 44-45 0.09421688975047005 1103 46-47 0.10291928505175463 1103 48-49 0.15662222147425808 1103 50-51 0.15205691992957782 1103 52-53 0.1186683566094544 1103 54-55 0.10866751319492352 1103 56-57 0.1403700074502794 1103 58-59 0.11394999750203283 1103 60-61 0.14345463412020365 1103 62-63 0.1296634097835323 1103 64-65 0.1168669293127067 1103 66-67 0.12216411461604793 1103 68-69 0.1827590904261669 1103 70-71 0.1535886246880409 1103 72-73 0.12144697289120643 1103 74-75 0.1405512572482479 1103 76-77 0.17182225409717944 1103 78-79 0.09546333017237174 1103 80-81 0.07450930001331812 1103 82-83 0.10031135944899461 1103 84-85 0.06924139005576535 1103 86-87 0.08757875904282031 1103 88-89 0.0900569504624329 1103 90-91 0.1154013484226013 1103 92-93 0.1128766952634841 1103 94-95 0.13496810604170761 1103 96-97 0.1416555072699346 1103 98-99 0.16386985262163734 1103 100 0.18857149932271255 1104 1 0.1295620660204797 1104 2 0.10934616654419216 1104 3 0.10894787405457151 1104 4 0.12499322160903148 1104 5 0.07880520588852846 1104 6 0.06803568960955175 1104 7 0.05378459174373518 1104 8 0.0027215751568334667 1104 9 -0.034079909499695304 1104 10-11 0.02975120716904911 1104 12-13 0.04167261983933912 1104 14-15 0.07918621414249571 1104 16-17 0.06375463231282197 1104 18-19 -7.448823382461001E-4 1104 20-21 0.0022389801612519022 1104 22-23 -0.052562997840922776 1104 24-25 -0.026199634867325017 1104 26-27 -0.03265862696466826 1104 28-29 -0.05522795149618531 1104 30-31 -0.06181340578439887 1104 32-33 -0.03518642150354623 1104 34-35 -0.05507049893640925 1104 36-37 -0.037927545575279 1104 38-39 7.763304420009831E-4 1104 40-41 -0.03386784058930914 1104 42-43 -0.057653658140033315 1104 44-45 -0.02958475025985763 1104 46-47 -0.0377540121151867 1104 48-49 -0.06392329164666677 1104 50-51 -0.07699218062858648 1104 52-53 -0.012729935647591617 1104 54-55 -0.03444012740302327 1104 56-57 -0.015592702051847596 1104 58-59 -0.02346121516849564 1104 60-61 -0.025829419473559767 1104 62-63 -0.03883218077244521 1104 64-65 -0.06310006363630549 1104 66-67 -0.07277894960769515 1104 68-69 0.016266640355269146 1104 70-71 -0.06550031407325463 1104 72-73 -0.044939898058729 1104 74-75 -0.05169503953889176 1104 76-77 -0.051872621588373136 1104 78-79 -0.08981962227814932 1104 80-81 -0.03802130437050266 1104 82-83 -0.03303484918659194 1104 84-85 -0.05001395368805106 1104 86-87 -0.01613466736838376 1104 88-89 -0.11361258946775621 1104 90-91 -0.07173490791731396 1104 92-93 -0.0955952217737881 1104 94-95 -0.06380911235016384 1104 96-97 -0.0494918296257687 1104 98-99 -0.020382811416993718 1104 100 0.03497952644483604 1105 1 0.07941717448728625 1105 2 0.05376614476632113 1105 3 0.07718649062476857 1105 4 0.08912956379388959 1105 5 0.06086661569079865 1105 6 -0.004780154633031941 1105 7 0.021122063459955598 1105 8 0.06189626336504972 1105 9 0.08019748505111579 1105 10-11 0.06532782082992128 1105 12-13 0.04452256076229588 1105 14-15 0.07380053498878425 1105 16-17 0.10528322383752453 1105 18-19 0.06663857854545796 1105 20-21 0.0692896436052024 1105 22-23 5.388332443203581E-4 1105 24-25 0.07771880531193887 1105 26-27 0.001354553639430378 1105 28-29 0.031573588506425665 1105 30-31 0.05948515728908177 1105 32-33 0.035057561250006586 1105 34-35 0.10678745695476977 1105 36-37 0.08785129374209788 1105 38-39 0.08646278361730708 1105 40-41 0.08467567694493283 1105 42-43 -0.04821634388214591 1105 44-45 0.0541049393596893 1105 46-47 -0.01113233822899673 1105 48-49 0.015415692959706462 1105 50-51 -0.06404147953034567 1105 52-53 0.019449811709840503 1105 54-55 0.05750765632139121 1105 56-57 0.02501486045322565 1105 58-59 0.04587307721584466 1105 60-61 0.07171544161157328 1105 62-63 0.06377205929722862 1105 64-65 0.01987227673294001 1105 66-67 0.05283969843612368 1105 68-69 0.07617926424497057 1105 70-71 0.13186427196528427 1105 72-73 0.11735126178376731 1105 74-75 0.09716275972190402 1105 76-77 0.1370747951312694 1105 78-79 0.03260679704271752 1105 80-81 0.07615599158948072 1105 82-83 0.0867979506144323 1105 84-85 0.04176512596922066 1105 86-87 0.057416288995995046 1105 88-89 0.003752585579462675 1105 90-91 0.05237306432694311 1105 92-93 -0.005972043368881685 1105 94-95 0.03494846859213041 1105 96-97 0.07266865439589054 1105 98-99 0.13395575544425853 1105 100 0.1068835466194038 1106 1 0.10037237381743225 1106 2 0.05644510404519565 1106 3 0.051563687726069674 1106 4 0.07853022456254877 1106 5 0.05838271693871633 1106 6 0.008961811688763532 1106 7 0.005349940445725565 1106 8 0.0035874301479381643 1106 9 0.006099006293176501 1106 10-11 -0.0031408202593823376 1106 12-13 -0.02250101928253656 1106 14-15 -0.020103604710790535 1106 16-17 -0.008421283815017944 1106 18-19 0.013038649508132494 1106 20-21 -0.00735892563686491 1106 22-23 0.010219273790276873 1106 24-25 0.04463176882835285 1106 26-27 -0.008664488347136512 1106 28-29 0.004287271310268181 1106 30-31 -0.02757948649701092 1106 32-33 -0.013479048416137118 1106 34-35 0.05235448839658119 1106 36-37 0.025549590217202933 1106 38-39 0.032577470099681705 1106 40-41 -0.023905477217965654 1106 42-43 -0.038227668422976535 1106 44-45 -0.04662262266285211 1106 46-47 -0.06896981757186893 1106 48-49 -0.004936923745731292 1106 50-51 0.008381723570629163 1106 52-53 0.02695067783601246 1106 54-55 -0.03411528350775228 1106 56-57 -0.04078003288760357 1106 58-59 -0.005577221561850365 1106 60-61 0.009541717211128287 1106 62-63 0.00852209348981603 1106 64-65 0.013399322546590042 1106 66-67 0.011727906226163043 1106 68-69 -0.002804150468605826 1106 70-71 -0.00488394185470753 1106 72-73 -0.011682590329428422 1106 74-75 6.090882392939534E-4 1106 76-77 -0.003427505362253669 1106 78-79 -0.054379680352710125 1106 80-81 -0.024548433947870762 1106 82-83 -0.03457464027910362 1106 84-85 -0.04094427557039637 1106 86-87 -0.020940859402578837 1106 88-89 -0.03464344372363115 1106 90-91 -0.01188725553487302 1106 92-93 -0.012692716603286414 1106 94-95 0.026166962693693563 1106 96-97 0.021753555177465245 1106 98-99 0.0524284124315173 1106 100 0.0609530103803948 1107 1 0.01176748161076091 1107 2 0.0032179690084106483 1107 3 -0.018534023856158655 1107 4 -0.008800775254840687 1107 5 -0.0033860510946581712 1107 6 -0.07303448992090011 1107 7 -0.04733459852402433 1107 8 -0.08338082594243446 1107 9 -0.0413298356387628 1107 10-11 -0.056578584520728725 1107 12-13 -0.05368942912020458 1107 14-15 -0.0983133397844469 1107 16-17 -0.056192444571514955 1107 18-19 -0.0636069880775807 1107 20-21 -0.07929721941070511 1107 22-23 -0.07520464508115765 1107 24-25 -0.06647516104049345 1107 26-27 -0.08686439499561516 1107 28-29 -0.09969698565300433 1107 30-31 -0.15236193777784734 1107 32-33 -0.10510823751279474 1107 34-35 -0.059544023842043714 1107 36-37 -0.09056261348571581 1107 38-39 -0.03727875275093595 1107 40-41 -0.08944460597240322 1107 42-43 -0.11517609333512269 1107 44-45 -0.12700600503168857 1107 46-47 -0.15072806940106886 1107 48-49 -0.11428868145787874 1107 50-51 -0.09711307379003387 1107 52-53 -0.13735576361082735 1107 54-55 -0.11413253631288 1107 56-57 -0.1007760574297123 1107 58-59 -0.10101430588603932 1107 60-61 -0.0787461645133476 1107 62-63 -0.09489029065437649 1107 64-65 -0.07758297755145804 1107 66-67 -0.0960754064670084 1107 68-69 -0.1323580738365493 1107 70-71 -0.05597270968861778 1107 72-73 -0.07072088650527064 1107 74-75 -0.09217367267248733 1107 76-77 -0.07380528476714687 1107 78-79 -0.07242335123179089 1107 80-81 -0.060462164456751566 1107 82-83 -0.07102629699710405 1107 84-85 -0.08639072417236093 1107 86-87 -0.06256449491770155 1107 88-89 -0.11158357181832912 1107 90-91 -0.059090812301903384 1107 92-93 -0.09120229185286277 1107 94-95 -0.06157939368401699 1107 96-97 -0.050982731236317136 1107 98-99 -0.03979020480015549 1107 100 -0.010449181905244131 1108 1 -0.06858858240274301 1108 2 -0.04783594478351105 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-0.27534072830102474 2215 12-13 -0.31253948882427807 2215 14-15 -0.36827942967580185 2215 16-17 -0.3107250175800118 2215 18-19 -0.3517863990915444 2215 20-21 -0.4870510065294056 2215 22-23 -0.4485922005105891 2215 24-25 -0.3739501078452889 2215 26-27 -0.4356505621576474 2215 28-29 -0.41102104857785093 2215 30-31 -0.4472940418621505 2215 32-33 -0.42920861008881417 2215 34-35 -0.4655837423609128 2215 36-37 -0.5682867671689493 2215 38-39 -0.49353827659545857 2215 40-41 -0.4989143373661449 2215 42-43 -0.471656028331239 2215 44-45 -0.4405305048942978 2215 46-47 -0.446450586379882 2215 48-49 -0.40074574431832843 2215 50-51 -0.4384807685114325 2215 52-53 -0.4913734624320014 2215 54-55 -0.5017056440551855 2215 56-57 -0.4326613304017286 2215 58-59 -0.45814247945810394 2215 60-61 -0.4120166675436394 2215 62-63 -0.4097025336990541 2215 64-65 -0.4742821934504775 2215 66-67 -0.4727692179112424 2215 68-69 -0.4129508993140405 2215 70-71 -0.3850829646526108 2215 72-73 -0.4394038991336444 2215 74-75 -0.4592800878860217 2215 76-77 -0.39552370179931273 2215 78-79 -0.4851788263316621 2215 80-81 -0.476574460663727 2215 82-83 -0.6101665233703386 2215 84-85 -0.5930699211889916 2215 86-87 -0.5872380678548197 2215 88-89 -0.6220605579976954 2215 90-91 -0.5941950892244208 2215 92-93 -0.528896401197418 2215 94-95 -0.553684976621934 2215 96-97 -0.5790102818998477 2215 98-99 -0.6484371893488188 2215 100 -0.6753666461954424 2216 1 -0.39463538230630846 2216 2 -0.37637394727149953 2216 3 -0.3699257257318074 2216 4 -0.3282761524349809 2216 5 -0.3383188085113389 2216 6 -0.29955527893795875 2216 7 -0.28134061799516275 2216 8 -0.314382490573486 2216 9 -0.4968672117413462 2216 10-11 -0.4109238958464445 2216 12-13 -0.28364469983666396 2216 14-15 -0.4234415218494618 2216 16-17 -0.5402523816800553 2216 18-19 -0.5052993353423219 2216 20-21 -0.44514838142455204 2216 22-23 -0.528280844541186 2216 24-25 -0.5001110588363531 2216 26-27 -0.5049200278537569 2216 28-29 -0.4101864965633908 2216 30-31 -0.525131925216094 2216 32-33 -0.5229852411953431 2216 34-35 -0.4672168422547571 2216 36-37 -0.5380102035042356 2216 38-39 -0.4967475258732179 2216 40-41 -0.5061078360143298 2216 42-43 -0.460745547052106 2216 44-45 -0.5685378512619081 2216 46-47 -0.5994991447471563 2216 48-49 -0.5115778875532726 2216 50-51 -0.5418352314667345 2216 52-53 -0.5626266957476602 2216 54-55 -0.5024893332396942 2216 56-57 -0.4800251259957804 2216 58-59 -0.5172720637491608 2216 60-61 -0.43090437439038 2216 62-63 -0.4367756573753283 2216 64-65 -0.5806286937547469 2216 66-67 -0.5320827197726956 2216 68-69 -0.4180149610953947 2216 70-71 -0.48551484909844334 2216 72-73 -0.4650995419006847 2216 74-75 -0.4362866619558119 2216 76-77 -0.4134205766285106 2216 78-79 -0.5038612499654676 2216 80-81 -0.45973050006360694 2216 82-83 -0.5001207564009462 2216 84-85 -0.5205289509151854 2216 86-87 -0.5359741579299353 2216 88-89 -0.5783142375858077 2216 90-91 -0.573988031905273 2216 92-93 -0.5341678387838407 2216 94-95 -0.6230032710449542 2216 96-97 -0.5737856852035854 2216 98-99 -0.571020877779759 2216 100 -0.5891914630828978 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 758647.0 3 169442.0 4 91234.0 5 81760.0 6 86359.0 7 90250.0 8 90672.0 9 97552.0 10 110614.0 11 118539.0 12 121329.0 13 125809.0 14 129782.0 15 132084.0 16 135986.0 17 139183.0 18 143486.0 19 146964.0 20 153681.0 21 163956.0 22 181496.0 23 208870.0 24 251634.0 25 311096.0 26 387579.0 27 487291.0 28 607700.0 29 754926.0 30 951430.0 31 1219472.0 32 1589806.0 33 2141146.0 34 3066289.0 35 4794470.0 36 8461511.0 37 1.5096877E7 38 2.0021473E7 39 7368727.0 40 33049.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.55695612253365 29.94718048124789 23.173023461382698 25.322839934835756 2 21.994560715735183 29.1465972974095 26.334480251162297 22.524361735693024 3 23.07730121246955 26.900529899407143 25.142565916949234 24.87960297117407 4 23.07777397178114 26.04633945623336 25.489686708964708 25.386199863020792 5 22.949923153306944 26.787749853981552 26.491585724933252 23.770741267778245 6 23.67258118376571 26.25388671636044 25.72181764403262 24.351714455841226 7 23.809877435136737 26.207303447122314 26.336559455364622 23.64625966237633 8 22.74002904979688 26.709624476589678 26.929052165607953 23.621294308005485 9 22.955495700709 26.303492100458147 26.878135145276254 23.8628770535566 10-11 23.0752737644045 26.536642788224402 26.784265640883646 23.603817806487452 12-13 23.084670325192334 26.35567633692156 26.910841023344766 23.648812314541342 14-15 23.182385889363516 26.31200605372458 26.923344278336923 23.582263778574976 16-17 23.292794097954776 26.196954366144237 26.84902641680032 23.661225119100667 18-19 23.371641549163353 26.03472768462904 26.753414706176315 23.840216060031295 20-21 23.42297547299195 26.00018863519402 26.722573759932533 23.854262131881494 22-23 23.511685838911127 25.98337497546727 26.63141742619442 23.87352175942718 24-25 23.61563576890859 25.92052653853516 26.48844682088599 23.975390871670257 26-27 23.659792326658867 25.954887641455343 26.4556695357588 23.92965049612699 28-29 23.71626470754944 25.99359596871058 26.34373759838996 23.946401725350018 30-31 23.79499345271848 25.868717233180703 26.29884195847521 24.037447355625606 32-33 23.80506163511213 25.903122073485264 26.27928906522854 24.01252722617407 34-35 23.831675837127808 25.918150170338823 26.215992204004856 24.034181788528514 36-37 23.86598001982701 25.855227804652674 26.187056476702093 24.091735698818223 38-39 23.871540629448145 25.828499053029063 26.224260617967765 24.075699699555024 40-41 23.91897116883439 25.84165110755648 26.16503867146809 24.074339052141045 42-43 23.954270788208813 25.7470548294776 26.105893746684416 24.19278063562917 44-45 23.938302709644272 25.75889964802045 26.143075026017815 24.159722616317463 46-47 24.008654849043083 25.795531867593365 26.058466073060526 24.137347210303027 48-49 24.01204699277633 25.72355023406957 26.02632584903156 24.238076924122538 50-51 24.005579490854938 25.75565655385126 26.05037555959993 24.18838839569387 52-53 24.00489632377731 25.755122489160332 26.03350123107182 24.206479955990538 54-55 24.06890294495222 25.696976667642108 25.979571805558425 24.254548581847242 56-57 24.076109415289817 25.727164056244174 25.988903102162613 24.207823426303396 58-59 24.073361347643935 25.771710775925843 25.95711232612603 24.197815550304192 60-61 24.108944196452168 25.711014348895834 25.88685545883359 24.29318599581841 62-63 24.080556519506167 25.71023773871123 25.936662552584224 24.27254318919838 64-65 24.121317909623475 25.766234153235462 25.881720613096633 24.23072732404443 66-67 24.163017346908834 25.711667131313405 25.83522875404791 24.29008676772985 68-69 24.149322380414155 25.736146571553654 25.856395327986505 24.258135720045686 70-71 24.198102300778036 25.766592069517458 25.810258676221597 24.225046953482906 72-73 24.204983088416757 25.717304413024472 25.77894300452896 24.298769494029813 74-75 24.2165516264693 25.72624908889282 25.81851996256093 24.238679322076944 76-77 24.240933057197566 25.786182939052555 25.76228595005269 24.210598053697193 78-79 24.28022216698893 25.747626270320833 25.721001835396347 24.251149727293893 80-81 24.266206161933464 25.76620145545499 25.742352002489373 24.225240380122177 82-83 24.26004454518886 25.789067983105955 25.73064013314293 24.22024733856225 84-85 24.2756999367622 25.75136156967204 25.68646197882535 24.286476514740414 86-87 24.276884614649887 25.789755665723256 25.693156266685275 24.240203452941586 88-89 24.318424027109153 25.816341872732895 25.64859483854888 24.216639261609068 90-91 24.34699549224733 25.770231817310158 25.61093301599731 24.271839674445204 92-93 24.319427100110012 25.791109019559816 25.636758408953252 24.252705471376924 94-95 24.359783241511572 25.838763703223982 25.58375350915029 24.21769954611415 96-97 24.389752975909637 25.832779426468015 25.533900982036638 24.243566615585706 98-99 24.413349549020786 25.888436527865277 25.531359684715117 24.166854238398823 100 24.45367165994277 25.901207436162643 25.45151406929344 24.193606834601148 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 81946.0 1 52852.5 2 24588.0 3 23982.0 4 17989.0 5 9876.0 6 5320.0 7 4180.5 8 3990.0 9 3951.5 10 4062.5 11 4289.0 12 4645.5 13 5203.5 14 6161.5 15 7860.5 16 10677.0 17 15483.5 18 23525.0 19 36006.0 20 55042.0 21 81490.5 22 116983.5 23 163122.5 24 219779.0 25 287799.5 26 368573.0 27 460262.0 28 560406.0 29 668303.0 30 780741.5 31 900300.0 32 1027675.0 33 1160750.0 34 1300996.5 35 1446259.0 36 1600202.0 37 1768276.0 38 1944400.0 39 2111978.5 40 2253351.0 41 2355230.5 42 2408324.0 43 2414090.0 44 2378410.5 45 2310447.0 46 2213171.0 47 2107290.0 48 2007811.0 49 1914006.0 50 1834737.5 51 1768478.5 52 1715807.0 53 1685062.0 54 1681905.5 55 1697783.0 56 1720481.5 57 1731293.5 58 1715899.0 59 1678118.5 60 1619070.0 61 1541244.0 62 1449196.5 63 1342941.5 64 1223512.5 65 1091395.5 66 957498.5 67 831838.0 68 713992.0 69 602786.0 70 503314.0 71 417145.0 72 341578.0 73 277937.5 74 225482.5 75 183834.5 76 150844.5 77 124181.5 78 102602.5 79 84365.5 80 69185.0 81 56645.5 82 45523.0 83 36166.5 84 28488.0 85 21922.5 86 16392.5 87 11958.0 88 8721.0 89 6167.5 90 4358.0 91 3091.5 92 2156.5 93 1534.5 94 1085.0 95 800.0 96 609.0 97 453.5 98 344.0 99 272.0 100 504.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24169635704321119 2 0.18528580321770224 3 0.17990438506871326 4 0.16817283718347614 5 0.14509412842364394 6 0.20985277963412297 7 0.23139675637344287 8 0.2568676758698351 9 0.26657731991887434 10-11 0.2587896109230454 12-13 0.2725184224514905 14-15 0.2718144169374941 16-17 0.26186259499164 18-19 0.2597090421243248 20-21 0.2633445265986026 22-23 0.2757864160474621 24-25 0.2637451057360666 26-27 0.2878185742871758 28-29 0.3131303885374047 30-31 0.31390127457523087 32-33 0.2911956887378168 34-35 0.2868942150472984 36-37 0.29380966121128566 38-39 0.279799247477242 40-41 0.28469067778848944 42-43 0.27944865273127173 44-45 0.2850264884186658 46-47 0.3009940374816196 48-49 0.30079128389358867 50-51 0.2956471156028165 52-53 0.3035376094036889 54-55 0.3022851835942892 56-57 0.29573652430309405 58-59 0.2925403392695501 60-61 0.29921149552018056 62-63 0.2952634325976884 64-65 0.30235065610709083 66-67 0.29359423552400277 68-69 0.297737307973872 70-71 0.3013967286356256 72-73 0.2959674381116849 74-75 0.2995106978636291 76-77 0.30438312002599865 78-79 0.29483821326723453 80-81 0.30344820070341133 82-83 0.30900632423641344 84-85 0.3169636985611155 86-87 0.3119075309595929 88-89 0.3219684737601164 90-91 0.3349615995264352 92-93 0.30208454202280016 94-95 0.30217254271204974 96-97 0.30313280623314093 98-99 0.29464320373985753 100 0.3365850362417111 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7.1022171E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.00841149898262 #Duplication Level Percentage of deduplicated Percentage of total 1 76.62914610382987 56.712013776679015 2 16.338788513280285 24.184155673713956 3 4.658897866585473 10.343928913259697 4 1.4998634663013908 4.440100504252951 5 0.5047751445888462 1.8678803307594887 6 0.18811428062023616 0.8353223453386518 7 0.07418816580073073 0.38433838120546604 8 0.03510913027449289 0.20786967685808466 9 0.017350052148708734 0.11556448190553989 >10 0.050870025592811015 0.7489402077927844 >50 0.0024780952361593994 0.12046319000963153 >100 4.191557409946076E-4 0.03942251822481976 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.1542568728291902E-4 1.4080110279929348E-6 4.2240330839788046E-6 9.856077195950543E-6 0.0 2 2.224657424228837E-4 2.8160220559858696E-6 8.448066167957609E-6 1.2672099251936414E-5 2.8160220559858696E-6 3 2.309138085908413E-4 8.448066167957609E-6 9.856077195950543E-6 2.2528176447886957E-5 4.2240330839788046E-6 4 2.3654585270281304E-4 1.8304143363908154E-5 1.9712154391901086E-5 3.3792264671830436E-5 7.040055139964674E-6 5 2.4921795195474944E-4 2.5344198503872827E-5 1.9712154391901086E-5 4.505635289577391E-5 7.040055139964674E-6 6 2.647060732626717E-4 2.8160220559858695E-5 1.9712154391901086E-5 5.632044111971739E-5 1.2672099251936414E-5 7 2.759701614866152E-4 4.083231981179511E-5 2.2528176447886957E-5 5.913646317570326E-5 1.4080110279929347E-5 8 2.858262386825658E-4 4.224033083978804E-5 2.3936187475879892E-5 7.040055139964674E-5 1.8304143363908154E-5 9 2.9286629382253046E-4 5.0688397007745655E-5 2.8160220559858695E-5 7.603259551161847E-5 2.2528176447886957E-5 10-11 3.104664316724421E-4 6.617651831566793E-5 3.23842536438375E-5 1.0208079952948778E-4 2.3936187475879892E-5 12-13 3.2525054746636794E-4 8.307265065158316E-5 3.731229224181277E-5 1.1334488775343125E-4 2.5344198503872824E-5 14-15 3.4425869634427256E-4 9.152071681954075E-5 4.716836943776332E-5 1.2812900354735706E-4 2.7456215045862227E-5 16-17 3.6819488382015245E-4 9.996878298749838E-5 5.702444663371386E-5 1.4784115793925814E-4 2.8160220559858695E-5 18-19 4.0832319811795107E-4 1.1686491532341358E-4 7.040055139964674E-5 1.6544129578916985E-4 2.8160220559858695E-5 20-21 4.576035840977038E-4 1.2953701457535E-4 7.251256794163614E-5 1.745933674711239E-4 3.0976242615844566E-5 22-23 4.998439149374919E-4 1.429131193412829E-4 7.462458448362554E-5 2.006415714889932E-4 3.872030326980571E-5 24-25 5.702444663371385E-4 1.4995317448124757E-4 7.814461205360787E-5 2.1824170933890487E-4 4.505635289577391E-5 26-27 6.512051004467323E-4 1.619212682191875E-4 8.307265065158316E-5 2.3372983064682716E-4 4.9984391493749184E-5 28-29 7.173816187624003E-4 1.682573178451557E-4 8.65926782215655E-5 2.541459905527247E-4 5.632044111971739E-5 30-31 8.384705671697927E-4 1.7811339504110625E-4 9.08167113055443E-5 2.6541007877666823E-4 6.195248523168913E-5 32-33 9.673035762311462E-4 1.879694722370568E-4 9.574474990351955E-5 2.830102166265799E-4 6.336049625968206E-5 34-35 0.0010447441827707575 1.9782554943300733E-4 9.856077195950544E-5 2.935702993365269E-4 7.040055139964673E-5 36-37 0.001155273048468203 2.1190565971293668E-4 9.92647774735019E-5 3.0976242615844566E-4 7.321657345563261E-5 38-39 0.0012629858921096625 2.2598576999286602E-4 1.0278480504348424E-4 3.2525054746636794E-4 7.462458448362554E-5 40-41 0.0013953389287409983 2.442899133567742E-4 1.0771284364145951E-4 3.414426742882867E-4 7.532858999762201E-5 42-43 0.001545292103222246 2.520339740107353E-4 1.1123287121144184E-4 3.541147735402231E-4 7.814461205360787E-5 44-45 0.0017269255258333346 2.675220953186576E-4 1.1897693186540298E-4 3.6889888933414896E-4 8.236864513758668E-5 46-47 0.0019170070146123806 2.7597016148661526E-4 1.2531298149137118E-4 3.9142706578203587E-4 8.588867270756901E-5 48-49 0.0021746730327350874 2.9075427728054103E-4 1.2812900354735706E-4 4.062111815759617E-4 9.152071681954075E-5 50-51 0.002447827172165717 3.104664316724421E-4 1.358730642013182E-4 4.21699302883884E-4 9.574474990351957E-5 52-53 0.002785045813370025 3.2525054746636794E-4 1.429131193412829E-4 4.4352347381777443E-4 9.856077195950544E-5 54-55 0.003172248846068082 3.3792264671830434E-4 1.4784115793925814E-4 4.6675565577965786E-4 1.0841684915545598E-4 56-57 0.0035904281213819836 3.5974681765219485E-4 1.5488121307922283E-4 4.8646781017155894E-4 1.1756892083741005E-4 58-59 0.004042399661367716 3.759389444741136E-4 1.5980925167719809E-4 5.026599369934777E-4 1.2038494289339592E-4 60-61 0.004647844403404678 3.9213107129603233E-4 1.6684930681716277E-4 5.223720913853788E-4 1.2108894840739239E-4 62-63 0.005293417459739438 4.1113922017393695E-4 1.73185356443131E-4 5.27300129983354E-4 1.2460897597737472E-4 64-65 0.006033327254949726 4.294433635378451E-4 1.7952140606909917E-4 5.470122843752552E-4 1.274249980333606E-4 66-67 0.006871093816605522 4.5830758961170026E-4 1.8374543915307797E-4 5.639084167111704E-4 1.3305704214533235E-4 68-69 0.007881341729190453 4.773157384896049E-4 1.872654667230603E-4 5.793965380190926E-4 1.3657706971531466E-4 70-71 0.0090873031746664 4.815397715735837E-4 1.8867347775105325E-4 5.941806538130184E-4 1.422091138272864E-4 72-73 0.01051854638462122 4.885798267135484E-4 1.8867347775105325E-4 6.096687751209408E-4 1.422091138272864E-4 74-75 0.012309536412228232 5.104039976474388E-4 1.9500952737702145E-4 6.272689129708524E-4 1.4643314691126523E-4 76-77 0.014678514966826345 5.294121465253434E-4 1.9923356046100025E-4 6.448690508207641E-4 1.5206519102323694E-4 78-79 0.017816971548222597 5.498283064312411E-4 2.0416159905897553E-4 6.624691886706758E-4 1.5840124064920517E-4 80-81 0.02227191844079224 5.70948471851135E-4 2.069776211149614E-4 6.892213982025415E-4 1.6966532887314865E-4 82-83 0.02848828712938105 5.878446041870502E-4 2.0979364317094728E-4 7.187896297903932E-4 1.7529737298512037E-4 84-85 0.03674627180855961 6.005167034389867E-4 2.1049764868494373E-4 7.427258172662731E-4 1.8374543915307797E-4 86-87 0.04834405864613742 6.174128357749019E-4 2.1542568728291902E-4 7.631419771721706E-4 1.872654667230603E-4 88 0.05993762145063124 6.378289956807994E-4 2.224657424228837E-4 7.800381095080859E-4 1.9289751083503205E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGATC 7930 0.0 13.392223 3 TTCCGAT 10775 0.0 10.074663 2 CCGATCT 10720 0.0 10.038515 4 GATCGGA 16780 0.0 9.21716 94 AGATCGG 21145 0.0 8.381598 94 CGATCTA 4675 0.0 8.141187 5 TAGATCG 7230 0.0 7.7375216 94 ATCGGAA 17840 0.0 7.668163 94 CTTCCGA 18250 0.0 6.1048546 1 >>END_MODULE