FastQCFastQC Report
Wed 25 May 2016
SRR2049544_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049544_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71022171
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA169750.09.02598894
AGATCGG207300.08.59264494
TAGATCG76700.07.659519794
ATCGGAA180950.07.272544494
CTCGTAT71450.06.808304342-43
TATGCCG71200.06.765506746-47
CGTATGC71750.06.64837644-45
TCGTATG79350.06.01201342-43
Produced by FastQC (version 0.11.5)