##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049544_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 71022171 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24464709759435 34.0 31.0 34.0 30.0 34.0 2 32.42516872653752 34.0 31.0 34.0 30.0 34.0 3 32.48410330909203 34.0 31.0 34.0 30.0 34.0 4 35.937126140511815 37.0 35.0 37.0 35.0 37.0 5 36.000502533216 37.0 35.0 37.0 35.0 37.0 6 36.038359739805756 37.0 35.0 37.0 35.0 37.0 7 36.04061185344503 37.0 35.0 37.0 35.0 37.0 8 36.041107797169424 37.0 35.0 37.0 35.0 37.0 9 37.76805604830075 39.0 38.0 39.0 35.0 39.0 10-11 37.77117874923874 39.0 38.0 39.0 35.0 39.0 12-13 37.755984106709434 39.0 38.0 39.0 35.0 39.0 14-15 39.17928751431718 40.0 39.0 41.0 36.0 41.0 16-17 39.153707368365296 40.0 39.0 41.0 36.0 41.0 18-19 39.16152794737857 40.0 39.0 41.0 36.0 41.0 20-21 39.17387474680265 40.0 39.0 41.0 36.0 41.0 22-23 39.14860351143025 40.0 39.0 41.0 36.0 41.0 24-25 39.12583449469603 40.0 39.0 41.0 36.0 41.0 26-27 39.038938502738816 40.0 39.0 41.0 35.5 41.0 28-29 38.978355019589586 40.0 39.0 41.0 35.0 41.0 30-31 38.91695726958276 40.0 38.5 41.0 35.0 41.0 32-33 38.840110188971835 40.0 38.0 41.0 35.0 41.0 34-35 38.781160033815354 40.0 38.0 41.0 35.0 41.0 36-37 38.70850717165489 40.0 38.0 41.0 35.0 41.0 38-39 38.637722528926915 40.0 38.0 41.0 35.0 41.0 40-41 38.569325718021204 40.0 38.0 41.0 34.5 41.0 42-43 38.48753438556532 40.0 38.0 41.0 34.0 41.0 44-45 38.40013088307312 40.0 38.0 41.0 34.0 41.0 46-47 38.298962179007454 40.0 38.0 41.0 34.0 41.0 48-49 38.174858580428356 40.0 37.0 41.0 33.5 41.0 50-51 38.056818602461476 40.0 37.0 41.0 33.0 41.0 52-53 37.911268855749285 40.0 37.0 41.0 33.0 41.0 54-55 37.73686844210944 40.0 36.0 41.0 33.0 41.0 56-57 37.55818382403433 39.5 36.0 41.0 33.0 41.0 58-59 37.36752608562191 39.0 35.0 41.0 33.0 41.0 60-61 37.12691268618077 39.0 35.0 41.0 32.5 41.0 62-63 36.909746169263116 38.5 35.0 41.0 32.0 41.0 64-65 36.63497354931603 38.0 35.0 40.5 32.0 41.0 66-67 36.376272685328075 37.0 35.0 40.0 32.0 41.0 68-69 36.07308652955709 37.0 35.0 40.0 31.0 41.0 70-71 35.78204321577272 36.0 35.0 39.0 31.0 41.0 72-73 35.46792391772986 36.0 35.0 39.0 31.0 41.0 74-75 35.15985193553151 35.0 35.0 38.5 31.0 40.5 76-77 34.831260382057316 35.0 34.0 37.0 31.0 39.5 78-79 34.53222716185344 35.0 34.0 37.0 31.0 39.0 80-81 34.24384044807642 35.0 34.0 36.5 30.0 39.0 82-83 34.007297094593184 35.0 34.0 36.0 30.0 37.5 84-85 33.77840031812038 35.0 34.0 36.0 30.0 37.0 86-87 33.59339650431131 35.0 34.0 35.0 30.0 37.0 88-89 33.415658252688445 35.0 34.0 35.0 30.0 36.0 90-91 33.25195958174807 35.0 34.0 35.0 30.0 36.0 92-93 33.09474820897829 35.0 34.0 35.0 30.0 36.0 94-95 32.978965758453086 35.0 34.0 35.0 30.0 36.0 96-97 32.84586318123111 35.0 34.0 35.0 30.0 35.0 98-99 32.73494093133256 35.0 34.0 35.0 29.0 35.0 100 32.65408960815912 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25595353915726093 1101 2 0.22797575036177875 1101 3 0.21688720586615773 1101 4 0.187627997432962 1101 5 0.18759569846538682 1101 6 0.20419361015619586 1101 7 0.18101800870475415 1101 8 0.17676303705361818 1101 9 0.2470949992431386 1101 10-11 0.22695283065403515 1101 12-13 0.26577618495789324 1101 14-15 0.32113187237597174 1101 16-17 0.3326007003133711 1101 18-19 0.36853981994205043 1101 20-21 0.32479783642986604 1101 22-23 0.2907668705343056 1101 24-25 0.297131900938453 1101 26-27 0.33575510252863694 1101 28-29 0.33270617645750633 1101 30-31 0.34057193354458803 1101 32-33 0.35160789658588953 1101 34-35 0.3609045163869027 1101 36-37 0.3525172454846697 1101 38-39 0.38059692979170734 1101 40-41 0.3572194810562124 1101 42-43 0.3360977217443377 1101 44-45 0.3426770625017852 1101 46-47 0.36415622121661073 1101 48-49 0.38954746259413753 1101 50-51 0.34656618755331436 1101 52-53 0.30474999009160797 1101 54-55 0.30296782672940026 1101 56-57 0.35015474961748794 1101 58-59 0.35436920272930195 1101 60-61 0.3372325143405419 1101 62-63 0.3344048878618864 1101 64-65 0.37563236611991613 1101 66-67 0.3400540339959903 1101 68-69 0.31948631340114986 1101 70-71 0.3210064734188123 1101 72-73 0.3241041362015835 1101 74-75 0.32400459430119355 1101 76-77 0.34190236664375107 1101 78-79 0.3141544391218787 1101 80-81 0.3386236664237572 1101 82-83 0.3214288067937474 1101 84-85 0.28079644822874883 1101 86-87 0.2746161144946697 1101 88-89 0.3131038057228466 1101 90-91 0.3224328151127409 1101 92-93 0.3630031971245984 1101 94-95 0.3477364884976595 1101 96-97 0.3678241703537921 1101 98-99 0.3780097741037949 1101 100 0.3945445633220501 1102 1 0.12276061223078472 1102 2 0.1290813902669754 1102 3 0.11326298885605723 1102 4 0.149435570465279 1102 5 0.17663798706235667 1102 6 0.15780906913771275 1102 7 0.1471250780648674 1102 8 0.15893102043072105 1102 9 0.18859069280586738 1102 10-11 0.18831525889032008 1102 12-13 0.19067330997857113 1102 14-15 0.2701393124536082 1102 16-17 0.2831585794607676 1102 18-19 0.30509430438075213 1102 20-21 0.24613644126713297 1102 22-23 0.26991249911181114 1102 24-25 0.26525087107869183 1102 26-27 0.2267257398661897 1102 28-29 0.24639852473265478 1102 30-31 0.26200831353069276 1102 32-33 0.25320242842480667 1102 34-35 0.27253909303278334 1102 36-37 0.2602050049641065 1102 38-39 0.3068121807812574 1102 40-41 0.2986344167723871 1102 42-43 0.3123482381833256 1102 44-45 0.278844411540625 1102 46-47 0.2732900199252626 1102 48-49 0.2530285932101606 1102 50-51 0.28975575660313524 1102 52-53 0.2614744909681832 1102 54-55 0.24480540216384128 1102 56-57 0.3019560756095032 1102 58-59 0.26762380187437884 1102 60-61 0.2540285898159311 1102 62-63 0.287674449735853 1102 64-65 0.2850977495195508 1102 66-67 0.25881236818668896 1102 68-69 0.2507918357599692 1102 70-71 0.22752155608640834 1102 72-73 0.25476311516536043 1102 74-75 0.23315319746999563 1102 76-77 0.2761132456911781 1102 78-79 0.22923528637283397 1102 80-81 0.25983852541797603 1102 82-83 0.21612282858875176 1102 84-85 0.20946600976679264 1102 86-87 0.20962948219990807 1102 88-89 0.24326049878238365 1102 90-91 0.229699522565177 1102 92-93 0.2842129445288677 1102 94-95 0.27413034348099075 1102 96-97 0.2732838110407343 1102 98-99 0.26138794823069134 1102 100 0.25570731640706157 1103 1 0.14910428724304836 1103 2 0.15181843796918315 1103 3 0.14559409419827318 1103 4 0.12592781382590346 1103 5 0.11359015787687099 1103 6 0.1479693707065053 1103 7 0.11964501251614479 1103 8 0.13425759541959792 1103 9 0.18878785391010666 1103 10-11 0.17429413852791242 1103 12-13 0.1466287298528215 1103 14-15 0.19193832493337482 1103 16-17 0.20970666899038548 1103 18-19 0.1899980542210855 1103 20-21 0.1687705559092194 1103 22-23 0.2018631314764079 1103 24-25 0.19987083600604905 1103 26-27 0.20968051949429167 1103 28-29 0.1835739200748705 1103 30-31 0.2338956913049799 1103 32-33 0.20629872055295095 1103 34-35 0.1810904037572314 1103 36-37 0.18693960663830467 1103 38-39 0.2008903798592243 1103 40-41 0.21415234789046877 1103 42-43 0.22912687571719914 1103 44-45 0.18472048582145106 1103 46-47 0.21613119348329946 1103 48-49 0.2363647340942734 1103 50-51 0.23221173539190687 1103 52-53 0.1822738986931114 1103 54-55 0.17325766938770926 1103 56-57 0.169784361471379 1103 58-59 0.1494845254835937 1103 60-61 0.1839179470941943 1103 62-63 0.21209621829660108 1103 64-65 0.16543591514979994 1103 66-67 0.18628879437523693 1103 68-69 0.19307059341455357 1103 70-71 0.15602333487033349 1103 72-73 0.1833645064306353 1103 74-75 0.14499258058089026 1103 76-77 0.13072389625366299 1103 78-79 0.1293461382973149 1103 80-81 0.11404160046762968 1103 82-83 0.16411258344055568 1103 84-85 0.08544619262044506 1103 86-87 0.10181530034711272 1103 88-89 0.09980403952982897 1103 90-91 0.0943201060440444 1103 92-93 0.1501105192654677 1103 94-95 0.14521421862869488 1103 96-97 0.16570278262550886 1103 98-99 0.1841204025291887 1103 100 0.17158762661425442 1104 1 0.07849118219646556 1104 2 0.07536138360821809 1104 3 0.0753294544231764 1104 4 0.07453430741571054 1104 5 0.07210366879208152 1104 6 0.09027459095106138 1104 7 0.09901169210768757 1104 8 0.11397290795373749 1104 9 0.14504776582860046 1104 10-11 0.1290012042096791 1104 12-13 0.15280672761014102 1104 14-15 0.14845887347058806 1104 16-17 0.14939764913533082 1104 18-19 0.15171992740167184 1104 20-21 0.11422658477484049 1104 22-23 0.12562514252603307 1104 24-25 0.12001099328199416 1104 26-27 0.06743961942864019 1104 28-29 0.10665880010193263 1104 30-31 0.13626150490002686 1104 32-33 0.07752357485406947 1104 34-35 0.09670041637104276 1104 36-37 0.10437061490692656 1104 38-39 0.10416498200942215 1104 40-41 0.1055979932758433 1104 42-43 0.10261284651944891 1104 44-45 0.12978000479790097 1104 46-47 0.1397736958775866 1104 48-49 0.12684137518046867 1104 50-51 0.13645408591192876 1104 52-53 0.10103790514446587 1104 54-55 0.10529826650022756 1104 56-57 0.13900174026198187 1104 58-59 0.10157192795317371 1104 60-61 0.13971513699853944 1104 62-63 0.05893114869130045 1104 64-65 0.10236733064194681 1104 66-67 0.09380699927492486 1104 68-69 0.13686558647143698 1104 70-71 0.11655319422040122 1104 72-73 0.09789401527278585 1104 74-75 0.05456075368456936 1104 76-77 0.0965858864922211 1104 78-79 0.09422952805959284 1104 80-81 0.0989496567978847 1104 82-83 0.11553779461902991 1104 84-85 0.059147559252600956 1104 86-87 0.03040602046085894 1104 88-89 0.07747045532971697 1104 90-91 0.06571322487305764 1104 92-93 0.10335233350055262 1104 94-95 0.11031132736003713 1104 96-97 0.09044878582565019 1104 98-99 0.0538124197028651 1104 100 0.05619096845592253 1105 1 0.0299735656134672 1105 2 0.050958720484054254 1105 3 0.027058877975122186 1105 4 0.06527854173642567 1105 5 0.05789584405264492 1105 6 0.05533027420673875 1105 7 0.08546773080615822 1105 8 0.07863867799441238 1105 9 0.0825114650258385 1105 10-11 0.0767745157767834 1105 12-13 0.08424977930006605 1105 14-15 0.08956615857663763 1105 16-17 0.10820393953903817 1105 18-19 0.10568043035057428 1105 20-21 0.08472760202939611 1105 22-23 0.08195276054942724 1105 24-25 0.0788315116454612 1105 26-27 0.09048410388791694 1105 28-29 0.08652132731664608 1105 30-31 0.08758445985175456 1105 32-33 0.10261506923481534 1105 34-35 0.07568563624855784 1105 36-37 0.1098170955430362 1105 38-39 0.1018572323897402 1105 40-41 0.11313970957134956 1105 42-43 0.07662865596218182 1105 44-45 0.07493432784131215 1105 46-47 0.08655575386352865 1105 48-49 0.0787223434159543 1105 50-51 0.07755881403026166 1105 52-53 0.03816201033771449 1105 54-55 0.05914666036689198 1105 56-57 0.1165081925757292 1105 58-59 0.11087716652086499 1105 60-61 0.10207304087723656 1105 62-63 0.07204829580526706 1105 64-65 0.09683597388934118 1105 66-67 0.05701555742889042 1105 68-69 0.0786804180035432 1105 70-71 0.09364464288348984 1105 72-73 0.07104672435738735 1105 74-75 0.04484450075504043 1105 76-77 0.09423897647029378 1105 78-79 0.07548388117167093 1105 80-81 0.0898525412570308 1105 82-83 0.06549286491942752 1105 84-85 0.029171595792753635 1105 86-87 0.07631474247039449 1105 88-89 0.09411454999710145 1105 90-91 0.09664500565799727 1105 92-93 0.10561058700504589 1105 94-95 0.08963491149837921 1105 96-97 0.09510002398389616 1105 98-99 0.11905656182582192 1105 100 0.10574896223899088 1106 1 0.023014864774886234 1106 2 0.023203301066345716 1106 3 0.010469519124008286 1106 4 0.057524734089646756 1106 5 0.01372670646267693 1106 6 0.029120413220745434 1106 7 0.055124109858809334 1106 8 0.03725361996155385 1106 9 0.08136960156330986 1106 10-11 0.08743955687197769 1106 12-13 0.0641906207817371 1106 14-15 0.10073508700956069 1106 16-17 0.101743145444388 1106 18-19 0.1216258236807235 1106 20-21 0.06590043353059372 1106 22-23 0.06815831428236407 1106 24-25 0.0729109774928105 1106 26-27 0.06288813435588025 1106 28-29 0.059743294162679206 1106 30-31 0.0794093182846467 1106 32-33 0.09470381207449918 1106 34-35 0.09030747549456208 1106 36-37 0.06292585657092786 1106 38-39 0.07459592463558096 1106 40-41 0.08216996343850269 1106 42-43 0.061125783874707906 1106 44-45 0.0784718232765087 1106 46-47 0.0799635865819397 1106 48-49 0.0706845661826847 1106 50-51 0.06666908790499093 1106 52-53 0.05655224120619806 1106 54-55 0.055455357843300135 1106 56-57 0.0570056663039864 1106 58-59 0.048887488029116355 1106 60-61 0.05952946746187848 1106 62-63 0.06172775567526401 1106 64-65 0.06335421724213575 1106 66-67 0.06785196174366348 1106 68-69 0.04255726620965561 1106 70-71 0.023142279417619704 1106 72-73 0.007231075507306173 1106 74-75 0.005306511455280827 1106 76-77 0.05526163201005829 1106 78-79 0.03972514180984632 1106 80-81 0.03951624988563651 1106 82-83 0.034250928067052655 1106 84-85 -0.0161406852975432 1106 86-87 0.05396796239732282 1106 88-89 -0.008692086918188124 1106 90-91 0.03616819485920786 1106 92-93 0.06178086402814387 1106 94-95 -0.017998385342558265 1106 96-97 0.007899631704411547 1106 98-99 -4.878453214729461E-5 1106 100 -0.01679402337732938 1107 1 -0.03458707758564117 1107 2 -0.043025015757841345 1107 3 -0.03929179051816334 1107 4 0.018252348010989294 1107 5 -0.006490200847274252 1107 6 0.026069616987740574 1107 7 0.019869543411672907 1107 8 0.02226952887505007 1107 9 0.021431028891861104 1107 10-11 0.030527411008861804 1107 12-13 0.024265138337213443 1107 14-15 0.017843583724918233 1107 16-17 0.006591390647741946 1107 18-19 -0.017731514692904682 1107 20-21 -0.010702546672064273 1107 22-23 0.006584007878544185 1107 24-25 -0.00425523754235968 1107 26-27 0.0037705703136694524 1107 28-29 0.021842489786799035 1107 30-31 0.009026957826968385 1107 32-33 0.03286776205911224 1107 34-35 -0.025386639484658247 1107 36-37 0.004386768122792262 1107 38-39 6.038733233708626E-4 1107 40-41 -0.03566242572301803 1107 42-43 -0.01320760266077059 1107 44-45 0.00483211902560754 1107 46-47 -0.029885037324774544 1107 48-49 0.008745552221604669 1107 50-51 -0.0310368566008421 1107 52-53 -0.03557762062203551 1107 54-55 -0.013681820422803526 1107 56-57 0.016967366195984823 1107 58-59 0.016529390490639173 1107 60-61 0.004198772342824952 1107 62-63 0.005918420774683852 1107 64-65 0.016339022933536285 1107 66-67 0.0120849697547456 1107 68-69 0.008419469151554893 1107 70-71 -0.012091386907137291 1107 72-73 0.008250197175136975 1107 74-75 0.009079332865752576 1107 76-77 0.029774438887876897 1107 78-79 0.010294150674234004 1107 80-81 0.019279851251383207 1107 82-83 0.01961755491708317 1107 84-85 -0.02144818889663469 1107 86-87 -0.007105586123145713 1107 88-89 -0.017195521898969446 1107 90-91 -0.006734984256191012 1107 92-93 -0.001934836260254258 1107 94-95 -0.03852923148836851 1107 96-97 -0.002883812438192024 1107 98-99 -0.009100292271988053 1107 100 -0.014740766913547532 1108 1 -0.11073243453039083 1108 2 -0.07898264515548448 1108 3 -0.07100339505247177 1108 4 -0.006820716068247634 1108 5 -0.008473414333899143 1108 6 -0.0027901858541383717 1108 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-0.17633071651602705 2214 32-33 -0.16754796427686358 2214 34-35 -0.2167821320976273 2214 36-37 -0.19213962983879895 2214 38-39 -0.20803412760046314 2214 40-41 -0.22847789270909402 2214 42-43 -0.17008850456960545 2214 44-45 -0.16674033128165178 2214 46-47 -0.17412790275502488 2214 48-49 -0.16851842874602596 2214 50-51 -0.18246495214440728 2214 52-53 -0.1596300941447808 2214 54-55 -0.15456865223023897 2214 56-57 -0.12666415178718182 2214 58-59 -0.1414186201472063 2214 60-61 -0.16832757983356572 2214 62-63 -0.12697233747063308 2214 64-65 -0.12319913161107365 2214 66-67 -0.1508137643012546 2214 68-69 -0.16333405392128242 2214 70-71 -0.13371040322459748 2214 72-73 -0.1402226880161379 2214 74-75 -0.12269257748701534 2214 76-77 -0.1172646534209889 2214 78-79 -0.10975052088728177 2214 80-81 -0.0899408930950969 2214 82-83 -0.1225848925929256 2214 84-85 -0.1395906089953698 2214 86-87 -0.13012129617106893 2214 88-89 -0.13235865085059118 2214 90-91 -0.1127296743676709 2214 92-93 -0.09942115504609461 2214 94-95 -0.0745574413701462 2214 96-97 -0.08194608765345635 2214 98-99 -0.09731859633831874 2214 100 -0.1356664281092108 2215 1 -0.34917709980764045 2215 2 -0.2322546788146198 2215 3 -0.20730842914270653 2215 4 -0.12084866855571619 2215 5 -0.12037064941100795 2215 6 -0.1520526634108137 2215 7 -0.13960960424108038 2215 8 -0.14447153638778332 2215 9 -0.14468177747749422 2215 10-11 -0.1418080689695742 2215 12-13 -0.1805616273216586 2215 14-15 -0.24908698664724938 2215 16-17 -0.25948890548934145 2215 18-19 -0.25795879189687554 2215 20-21 -0.2726825503955439 2215 22-23 -0.294504451113923 2215 24-25 -0.23645116261182864 2215 26-27 -0.24803510402602313 2215 28-29 -0.26194589797108847 2215 30-31 -0.2682352262766443 2215 32-33 -0.27873415957891723 2215 34-35 -0.2925164966989584 2215 36-37 -0.26883872511615436 2215 38-39 -0.25388502929680357 2215 40-41 -0.31240064675547785 2215 42-43 -0.2989146713319144 2215 44-45 -0.28713423666027893 2215 46-47 -0.2767445004812501 2215 48-49 -0.2774832689009159 2215 50-51 -0.3249093618378822 2215 52-53 -0.29504406818603 2215 54-55 -0.2562765426559821 2215 56-57 -0.2799316817295434 2215 58-59 -0.2893466080076905 2215 60-61 -0.3011092008191625 2215 62-63 -0.27746795363855625 2215 64-65 -0.27758435430553163 2215 66-67 -0.2817705125419394 2215 68-69 -0.2598943296984615 2215 70-71 -0.2628454546319645 2215 72-73 -0.28134927644286734 2215 74-75 -0.23382850463551108 2215 76-77 -0.2328883204574339 2215 78-79 -0.21376461945742875 2215 80-81 -0.18897299262718548 2215 82-83 -0.2653938140108778 2215 84-85 -0.29442470420554656 2215 86-87 -0.298007313150336 2215 88-89 -0.2958187769933289 2215 90-91 -0.30231552873143386 2215 92-93 -0.31248042180430957 2215 94-95 -0.3052120929746849 2215 96-97 -0.3083621847223341 2215 98-99 -0.3312427852846582 2215 100 -0.3208588626912885 2216 1 -0.3124041703709999 2216 2 -0.2445537292420923 2216 3 -0.2372461836051727 2216 4 -0.15524881539290192 2216 5 -0.12976995048445872 2216 6 -0.17419385234163798 2216 7 -0.1597109687035072 2216 8 -0.17302620316237238 2216 9 -0.20410644025750457 2216 10-11 -0.2161865601621571 2216 12-13 -0.2590782728853398 2216 14-15 -0.2919145086597581 2216 16-17 -0.3262710574751537 2216 18-19 -0.26021646995158676 2216 20-21 -0.2853456870005431 2216 22-23 -0.28685322079357434 2216 24-25 -0.25012627894410855 2216 26-27 -0.3164471473719459 2216 28-29 -0.3078042227059754 2216 30-31 -0.3045857214943055 2216 32-33 -0.33391696488195777 2216 34-35 -0.34161360423937737 2216 36-37 -0.3270185919799644 2216 38-39 -0.32761057207503796 2216 40-41 -0.3066439718103524 2216 42-43 -0.2956228881778671 2216 44-45 -0.3059849022361547 2216 46-47 -0.3162055576332605 2216 48-49 -0.30639818172440414 2216 50-51 -0.33843233158892616 2216 52-53 -0.3689069691250495 2216 54-55 -0.33900405385132615 2216 56-57 -0.29678250043073007 2216 58-59 -0.33413280392487366 2216 60-61 -0.30005753624450904 2216 62-63 -0.32052618388042475 2216 64-65 -0.2833579186852404 2216 66-67 -0.31912361930339017 2216 68-69 -0.3041386421145873 2216 70-71 -0.28917233011117816 2216 72-73 -0.3424541095335769 2216 74-75 -0.331932934065442 2216 76-77 -0.28231847893491135 2216 78-79 -0.2700122706585759 2216 80-81 -0.27394079450592557 2216 82-83 -0.31638997656838797 2216 84-85 -0.3233490361820017 2216 86-87 -0.3008464630013208 2216 88-89 -0.3025517380233538 2216 90-91 -0.2972998876477746 2216 92-93 -0.32962224824538566 2216 94-95 -0.35561550280266374 2216 96-97 -0.38677931414179767 2216 98-99 -0.3942921025047994 2216 100 -0.3759085071859687 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8063.0 3 1458.0 4 1279.0 5 1833.0 6 3399.0 7 6938.0 8 14007.0 9 22409.0 10 30954.0 11 37099.0 12 42160.0 13 46426.0 14 50017.0 15 54153.0 16 59054.0 17 63938.0 18 70025.0 19 77593.0 20 86096.0 21 97632.0 22 114335.0 23 138453.0 24 176375.0 25 230618.0 26 306689.0 27 411756.0 28 550473.0 29 727404.0 30 952734.0 31 1241366.0 32 1621360.0 33 2157404.0 34 3049833.0 35 4753776.0 36 8573775.0 37 1.5897991E7 38 2.1397941E7 39 7907611.0 40 37744.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.938730199916975 27.18285108235829 25.187869326741886 23.690549390982856 2 23.626399543149535 26.679434179242058 25.562904334641672 24.131261942966738 3 23.54621413364162 25.701057446014445 26.3940377602286 24.358690660115336 4 24.082123064928428 25.530223198256735 26.280379333700072 24.107274403114765 5 24.04814682607264 25.881498305279614 26.217938434691167 23.852416433956574 6 23.890486253933297 25.688177006022528 25.95450369997842 24.466833040065755 7 23.720632251582398 25.900916208264036 26.418916425407495 23.95953511474607 8 23.37528510084629 26.006692677553833 26.492066189424463 24.125956032175417 9 23.364851737183855 25.816841609699875 26.62523862748256 24.193068025633714 10-11 23.363421965797134 26.074778818011634 26.645465399811556 23.916333816379677 12-13 23.23495081556997 25.92293327670876 26.77970728323695 24.06240862448432 14-15 23.265607982861685 25.896960314493434 26.783328862896997 24.05410283974788 16-17 23.35737385442639 25.83017703021216 26.744477439305538 24.06797167605592 18-19 23.38026961486254 25.718557412474098 26.70873147275018 24.19244149991318 20-21 23.443277809083412 25.706242773982108 26.658947556264994 24.191531860669485 22-23 23.520209613693712 25.747317093054622 26.565968870736008 24.16650442251566 24-25 23.573541167465912 25.70719085216518 26.50604699827342 24.213220982095493 26-27 23.663851629169148 25.717407498078476 26.43834341182049 24.180397460931882 28-29 23.725668036728536 25.760658209829867 26.377441970500964 24.136231782940634 30-31 23.764999283031838 25.68669222179738 26.352024312395283 24.196284182775496 32-33 23.804553582195297 25.69699724057238 26.296881840591674 24.20156733664065 34-35 23.84266798567086 25.711604511081642 26.27173117434754 24.173996328899953 36-37 23.870294814306707 25.677262271772992 26.24145033723071 24.210992576689595 38-39 23.89315347315113 25.665267787464003 26.23067796649191 24.210900772892956 40-41 23.941380663535057 25.69134979191047 26.180959677363713 24.186309867190765 42-43 23.966757880115313 25.623108279384986 26.14111816263216 24.26901567786754 44-45 23.97553933038244 25.613540657018486 26.14282925211849 24.26809076048059 46-47 24.016655573516008 25.666504091657245 26.09851074021174 24.218329594615014 48-49 24.048625571041914 25.613027536339057 26.05715654144694 24.281190351172086 50-51 24.048689790010258 25.627402900918728 26.051122651839385 24.27278465723163 52-53 24.060487578205652 25.647038673776667 26.04475816753234 24.24771558048534 54-55 24.0902922578893 25.607377833005497 26.020649844160932 24.281680064944272 56-57 24.10183705585559 25.61705632926598 25.999653444667352 24.281453170211076 58-59 24.13360829616099 25.649051039753118 25.983167737697393 24.234172926388496 60-61 24.134630235892523 25.616596771493434 25.950421151134773 24.29835184147927 62-63 24.129017566515675 25.603702652199896 25.964613271687043 24.30266650959739 64-65 24.148365183802714 25.66802609271787 25.93715461712059 24.246454106358826 66-67 24.156679227997675 25.63244336585585 25.90832724575625 24.30255016039023 68-69 24.175210336890256 25.639484895811492 25.89134334151725 24.293961425781006 70-71 24.206263181883273 25.671121983048057 25.870529142390318 24.252085692678353 72-73 24.20948944197568 25.659987184815336 25.842269116610968 24.288254256598023 74-75 24.22202511001285 25.661343099922355 25.853257281058983 24.263374509005807 76-77 24.24508773942686 25.7036407059542 25.827981486045964 24.22329006857297 78-79 24.263394718558835 25.67886172837533 25.803303293525214 24.25444025954062 80-81 24.268872841388745 25.692848094972405 25.801627883852092 24.23665117978676 82-83 24.261729937637487 25.721912233439692 25.802227807599486 24.21413002132333 84-85 24.257322169619798 25.7005474634948 25.78832202397658 24.253808342908822 86-87 24.25724514925238 25.727773098241276 25.7727567244787 24.242225028027644 88-89 24.28326115887588 25.757801530891307 25.759448206210887 24.199489104021925 90-91 24.286176107465106 25.740808316757313 25.737081981756617 24.23593359402096 92-93 24.281415504324382 25.747687069976845 25.745334166288465 24.225563259410315 94-95 24.288174276726068 25.790224981791393 25.721067573160898 24.200533168321638 96-97 24.301020320445723 25.801191394944944 25.690605836623437 24.207182447985897 98-99 24.308143452796322 25.84079847636075 25.675829362958957 24.17522870788397 100 24.328757484488854 25.880779831080652 25.640073147234048 24.15038953719645 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 33.0 1 36.0 2 50.0 3 65.5 4 77.0 5 109.5 6 191.5 7 307.0 8 431.5 9 576.5 10 732.0 11 900.5 12 1164.0 13 1597.0 14 2209.5 15 3335.5 16 5398.5 17 8927.5 18 14918.0 19 24713.5 20 39746.5 21 61231.5 22 90638.5 23 129778.0 24 179819.0 25 241101.0 26 313453.5 27 397388.0 28 492514.5 29 597377.5 30 708651.0 31 829225.5 32 959240.5 33 1098766.5 34 1248773.0 35 1403170.0 36 1568860.0 37 1754058.5 38 1950145.5 39 2136493.5 40 2293965.5 41 2411720.5 42 2478868.5 43 2490715.5 44 2457207.5 45 2388204.5 46 2286461.5 47 2173730.0 48 2066524.5 49 1967990.0 50 1886099.5 51 1817672.5 52 1763566.0 53 1735411.5 54 1734905.5 55 1752984.0 56 1777127.0 57 1787402.0 58 1772421.0 59 1728905.5 60 1661889.5 61 1575205.5 62 1474723.0 63 1360863.5 64 1231398.5 65 1091782.0 66 951055.0 67 821077.5 68 700672.5 69 587788.5 70 487398.5 71 400590.5 72 325527.0 73 262473.0 74 210918.0 75 170207.0 76 138108.5 77 112800.0 78 91975.5 79 74518.0 80 60372.0 81 48515.5 82 38470.5 83 30104.0 84 22756.5 85 16808.0 86 12354.5 87 8610.0 88 5722.0 89 3673.0 90 2376.0 91 1532.0 92 801.5 93 421.5 94 249.5 95 147.5 96 96.0 97 53.0 98 33.0 99 17.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04674737414039343 2 3.44962701858269E-4 3 9.475914218392452E-4 4 0.0841202108564099 5 0.020123293612075023 6 0.0 7 0.0 8 5.237801024133717E-4 9 5.420842457772799E-4 10-11 0.0 12-13 0.0 14-15 2.956823158785163E-5 16-17 0.0 18-19 7.98342252871994E-4 20-21 2.3936187475879893E-4 22-23 1.316490311173394E-4 24-25 0.002199313225724964 26-27 0.003288409755877499 28-29 0.005756653087949114 30-31 0.011066966680024468 32-33 0.004357794131638133 34-35 0.005559531544030103 36-37 0.005144168290772187 38-39 0.007989758578345907 40-41 0.0031835129342920256 42-43 0.006079087613359496 44-45 0.003791069692870977 46-47 0.0022816818708625507 48-49 3.3651463569031137E-4 50-51 6.533171169887217E-4 52-53 0.0014227951437868606 54-55 9.38439350157291E-4 56-57 0.004518307388829327 58-59 0.0016572289799476842 60-61 9.792716699690862E-4 62-63 6.075567585789514E-4 64-65 0.0012665059196796447 66-67 0.0016438528751817514 68-69 0.001305930228463447 70-71 0.0019726234502181017 72-73 0.003541851740916227 74-75 8.201664238058845E-4 76-77 0.0011566810594961958 78-79 0.0023774266207660702 80-81 0.002288017920488519 82-83 0.005898158196262404 84-85 0.0038473901339906944 86-87 0.001089096530152535 88-89 3.900190547540429E-4 90-91 9.074631075414465E-4 92-93 0.004983655033580992 94-95 1.8937748326504974E-4 96-97 0.00479286953928795 98-99 0.003111704371864386 100 0.0029906154234569933 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7.1022171E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.6920777955684 #Duplication Level Percentage of deduplicated Percentage of total 1 75.81589757754138 55.87031022425024 2 17.060902699467295 25.14506737983533 3 4.7904953806900545 10.590646748193674 4 1.5041994565420733 4.4339033348620065 5 0.4952262231715407 1.8247124682181346 6 0.18029570600639083 0.7971819115937927 7 0.07041833904428683 0.36324916033604077 8 0.025379157299647195 0.14961942672892545 9 0.013787495904162015 0.0914426298698029 >10 0.042046472312934347 0.6602598285672554 >50 0.001290510343456379 0.05625788056183671 >100 5.907092295850381E-5 0.005478941608793501 >500 0.0 0.0 >1k 0.0 0.0 >5k 1.9107538102950685E-6 0.011870065374192136 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.1120165419894022E-4 0.0 2.8160220559858696E-6 5.632044111971739E-6 1.4080110279929348E-6 2 2.1401767625492608E-4 5.632044111971739E-6 4.2240330839788046E-6 1.6896132335915218E-5 4.2240330839788046E-6 3 2.1542568728291902E-4 1.1264088223943478E-5 4.2240330839788046E-6 3.23842536438375E-5 7.040055139964674E-6 4 2.2387375345087662E-4 2.112016541989402E-5 8.448066167957609E-6 5.0688397007745655E-5 1.1264088223943478E-5 5 2.3372983064682718E-4 3.0976242615844566E-5 8.448066167957609E-6 5.632044111971739E-5 1.1264088223943478E-5 6 2.3372983064682718E-4 3.3792264671830436E-5 8.448066167957609E-6 5.7728452147710325E-5 1.1264088223943478E-5 7 2.4076988578679184E-4 4.364834186778098E-5 8.448066167957609E-6 6.617651831566793E-5 1.4080110279929347E-5 8 2.4217789681478478E-4 4.928038597975272E-5 9.856077195950543E-6 7.462458448362554E-5 1.4080110279929347E-5 9 2.478099409267565E-4 5.632044111971739E-5 9.856077195950543E-6 8.025662859559728E-5 1.5488121307922283E-5 10-11 2.689301063466505E-4 6.969654588565027E-5 1.056008270994701E-5 9.71527609315125E-5 2.0416159905897553E-5 12-13 2.81602205598587E-4 9.152071681954075E-5 1.1968093737939946E-5 1.2108894840739238E-4 2.3936187475879892E-5 14-15 2.9145828279453754E-4 1.0700883812746304E-4 1.5488121307922283E-5 1.527691965372334E-4 2.675220953186576E-5 16-17 3.0765040961645623E-4 1.2179295392138886E-4 2.0416159905897553E-5 1.7177734541513803E-4 2.8160220559858695E-5 18-19 3.308825915783397E-4 1.344650531733253E-4 2.5344198503872824E-5 1.921935053210356E-4 3.0976242615844566E-5 20-21 3.759389444741136E-4 1.5206519102323694E-4 3.0976242615844566E-5 2.0416159905897553E-4 3.23842536438375E-5 22-23 4.146592477439193E-4 1.5980925167719809E-4 3.520027569982337E-5 2.2316974793688016E-4 3.3792264671830436E-5 24-25 4.730917054056261E-4 1.7529737298512037E-4 4.083231981179511E-5 2.3795386373080596E-4 3.449627018582691E-5 26-27 5.526443284872269E-4 1.8444944466707448E-4 4.364834186778098E-5 2.611860456926894E-4 3.942430878380217E-5 28-29 6.145968137189161E-4 1.94305521863025E-4 4.505635289577391E-5 2.724501339166329E-4 4.083231981179511E-5 30-31 7.490618668922412E-4 2.0697762111496142E-4 4.576035840977038E-5 2.970903269065092E-4 4.646436392376685E-5 32-33 8.624067546456726E-4 2.2950579756284836E-4 5.068839700774565E-5 3.1257844821443154E-4 4.857638046575625E-5 34-35 9.321033005313228E-4 2.456979243847671E-4 5.843245766170679E-5 3.2806656952235377E-4 5.843245766170679E-5 36-37 0.0010419281607147717 2.6681808980466115E-4 6.0544474203696196E-5 3.477787239142549E-4 6.195248523168913E-5 38-39 0.001147528987814242 2.7456215045862224E-4 6.195248523168913E-5 3.7030690036214187E-4 6.406450177367853E-5 40-41 0.0012812900354735707 2.8441822765457286E-4 6.899254037165382E-5 3.99171126435997E-4 6.68805238296644E-5 42-43 0.0014284271878988323 2.999063489624951E-4 7.110455691364321E-5 4.2873935802384866E-4 6.89925403716538E-5 44-45 0.0016023165498559597 3.1257844821443154E-4 7.392057896962907E-5 4.5408355652772146E-4 7.251256794163614E-5 46-47 0.001792398038635006 3.315865970923361E-4 7.603259551161847E-5 4.7097968886363666E-4 7.603259551161847E-5 48-49 0.0020437280071317447 3.5270676251223016E-4 7.884861756760434E-5 5.005479204514883E-4 7.744060653961141E-5 50-51 0.0023218101851603494 3.7664294998811004E-4 8.096063410959376E-5 5.188520638153965E-4 7.744060653961141E-5 52-53 0.002658324820850661 3.829789996140782E-4 8.44806616795761E-5 5.413802402632834E-4 8.025662859559727E-5 54-55 0.0030342637653247746 3.893150492400465E-4 8.870469476355488E-5 5.582763725991986E-4 8.236864513758668E-5 56-57 0.003438362930358747 3.970591098940076E-4 9.011270579154783E-5 5.927726427850256E-4 8.729668373556196E-5 58-59 0.0039001905475404292 4.139552422299228E-4 9.222472233353723E-5 6.174128357749019E-4 8.870469476355488E-5 60-61 0.004503523273035402 4.2381131942587335E-4 9.433673887552664E-5 6.364209846528065E-4 9.574474990351955E-5 62-63 0.005144872296286183 4.421154627897815E-4 9.856077195950544E-5 6.561331390447076E-4 9.785676644550897E-5 64-65 0.005875630019814516 4.681636668076508E-4 1.0278480504348424E-4 6.849973651185628E-4 1.0137679401549131E-4 66-67 0.006711284564928324 4.7872374951759785E-4 1.0419281607147717E-4 7.004854864264851E-4 1.0208079952948778E-4 68-69 0.007720828471999258 4.95619881853513E-4 1.0700883812746304E-4 7.194936353043897E-4 1.0419281607147718E-4 70-71 0.008926789917475208 5.111080031614352E-4 1.0841684915545598E-4 7.356857621263085E-4 1.0771284364145951E-4 72-73 0.01037281724322395 5.202600748433894E-4 1.0841684915545598E-4 7.490618668922412E-4 1.1123287121144185E-4 74-75 0.012179295392138885 5.357481961513117E-4 1.1052886569744538E-4 7.758140764241071E-4 1.1264088223943478E-4 76-77 0.014553905990848971 5.533483340012233E-4 1.1616090980941712E-4 7.990462583859904E-4 1.1475289878142418E-4 78-79 0.017705738677011154 5.674284442811527E-4 1.1827292635140652E-4 8.265024734318528E-4 1.1545690429542065E-4 80-81 0.022180397723972702 5.878446041870502E-4 1.1827292635140652E-4 8.588867270756901E-4 1.1616090980941712E-4 82-83 0.02840662248975746 6.096687751209408E-4 1.2038494289339592E-4 8.919749862335241E-4 1.1897693186540298E-4 84-85 0.03678640012285741 6.343089681108171E-4 1.2390497046337826E-4 9.130951516534182E-4 1.2390497046337826E-4 86-87 0.04847570767725476 6.497970894187394E-4 1.2883300906135352E-4 9.370313391292981E-4 1.274249980333606E-4 88 0.06016853525922208 6.603571721286864E-4 1.337610476593288E-4 9.560394880072027E-4 1.2953701457535E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 16975 0.0 9.025988 94 AGATCGG 20730 0.0 8.592644 94 TAGATCG 7670 0.0 7.6595197 94 ATCGGAA 18095 0.0 7.2725444 94 CTCGTAT 7145 0.0 6.8083043 42-43 TATGCCG 7120 0.0 6.7655067 46-47 CGTATGC 7175 0.0 6.648376 44-45 TCGTATG 7935 0.0 6.012013 42-43 >>END_MODULE