##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049543_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 85700132 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.806631021291775 33.0 31.0 34.0 30.0 34.0 2 31.95994492750606 34.0 31.0 34.0 30.0 34.0 3 31.998218929231054 34.0 31.0 34.0 30.0 34.0 4 35.5008562180511 37.0 35.0 37.0 33.0 37.0 5 35.49269236831514 37.0 35.0 37.0 33.0 37.0 6 35.51682290290988 37.0 35.0 37.0 33.0 37.0 7 35.506661389973125 37.0 35.0 37.0 33.0 37.0 8 35.4933160779729 37.0 35.0 37.0 33.0 37.0 9 37.14589325253315 39.0 37.0 39.0 33.0 39.0 10-11 37.11239753983109 39.0 37.0 39.0 33.0 39.0 12-13 37.07763446035299 39.0 37.0 39.0 33.0 39.0 14-15 38.37401988482351 40.0 38.0 41.0 33.0 41.0 16-17 38.37666216196726 40.0 38.0 41.0 33.0 41.0 18-19 38.36400251985609 40.0 38.0 41.0 33.5 41.0 20-21 38.329795472193666 40.0 38.0 41.0 34.0 41.0 22-23 38.25800246141978 40.0 38.0 41.0 34.0 41.0 24-25 38.20067775974954 40.0 38.0 41.0 33.0 41.0 26-27 38.11336743331971 40.0 38.0 41.0 33.0 41.0 28-29 38.009994278655256 40.0 38.0 41.0 33.0 41.0 30-31 37.901754842104566 40.0 38.0 41.0 33.0 41.0 32-33 37.791853651987374 40.0 38.0 41.0 33.0 41.0 34-35 37.67445117237392 40.0 37.5 41.0 32.0 41.0 36-37 37.561636241120375 40.0 37.0 41.0 32.0 41.0 38-39 37.42145671374229 40.0 37.0 41.0 32.0 41.0 40-41 37.27626134811554 40.0 37.0 41.0 31.0 41.0 42-43 37.127223497158674 40.0 36.5 41.0 31.0 41.0 44-45 36.96696928658173 40.0 36.0 41.0 31.0 41.0 46-47 36.79597099687081 39.0 36.0 41.0 30.5 41.0 48-49 36.732203685520574 39.0 35.5 41.0 30.5 41.0 50-51 35.64834321375375 38.0 34.5 40.0 29.5 40.5 52-53 35.735990167436384 38.5 34.5 39.5 29.5 40.5 54-55 36.359738214872294 39.0 35.0 40.5 30.0 41.0 56-57 36.3155556516529 39.0 35.0 41.0 30.0 41.0 58-59 36.11944356748482 39.0 35.0 41.0 30.0 41.0 60-61 36.19234863022148 39.0 35.0 41.0 30.0 41.0 62-63 36.02913910914396 38.0 35.0 41.0 30.0 41.0 64-65 35.78036810375041 37.5 35.0 40.0 30.0 41.0 66-67 35.49927495444231 37.0 35.0 40.0 30.0 41.0 68-69 35.18130911980393 36.5 34.0 40.0 29.0 41.0 70-71 34.8694637774887 36.0 34.0 39.0 29.0 41.0 72-73 34.52777392454891 36.0 34.0 39.0 29.0 41.0 74-75 34.18770190458983 35.0 34.0 38.5 29.0 40.5 76-77 33.84967442057149 35.0 34.0 37.0 29.0 39.5 78-79 33.529539814477765 35.0 34.0 37.0 28.0 39.0 80-81 33.21643292218033 35.0 33.0 36.5 28.0 39.0 82-83 32.91068244795702 35.0 33.0 36.0 27.0 37.5 84-85 32.64481585045867 35.0 33.0 36.0 27.0 37.0 86-87 32.40666767001012 35.0 33.0 35.0 27.0 37.0 88-89 32.193578727510015 35.0 33.0 35.0 26.0 36.0 90-91 32.00169360882665 35.0 33.0 35.0 26.0 36.0 92-93 31.81038369345802 35.0 33.0 35.0 26.0 36.0 94-95 31.618812389927243 35.0 33.0 35.0 25.0 36.0 96-97 31.413376942056516 35.0 33.0 35.0 25.0 35.0 98-99 31.175639128537163 35.0 32.5 35.0 24.0 35.0 100-101 30.394374001664314 34.5 31.0 35.0 20.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4632604898002697 1101 2 0.4997937713961811 1101 3 0.4996973635552209 1101 4 0.431515878798443 1101 5 0.4306303110901908 1101 6 0.43462525776674 1101 7 0.43816918188933585 1101 8 0.4553054235751688 1101 9 0.5308419909024309 1101 10-11 0.559317817414346 1101 12-13 0.5651007176896385 1101 14-15 0.6953597729978966 1101 16-17 0.6804160708898692 1101 18-19 0.6660095766452088 1101 20-21 0.6737581829114916 1101 22-23 0.6620393082947871 1101 24-25 0.6955441499615844 1101 26-27 0.6865898009175027 1101 28-29 0.6793883492151025 1101 30-31 0.7250639312526204 1101 32-33 0.7042001259137933 1101 34-35 0.7173946736944572 1101 36-37 0.7178195779641285 1101 38-39 0.7039269162767496 1101 40-41 0.7259328553841655 1101 42-43 0.7129362643539849 1101 44-45 0.7156091306073549 1101 46-47 0.7275824695314483 1101 48-49 0.6777854428401469 1101 50-51 0.6405148344723699 1101 52-53 0.6645370525037393 1101 54-55 0.7091970380333166 1101 56-57 0.7107294701580429 1101 58-59 0.6936276040015557 1101 60-61 0.7216114702177734 1101 62-63 0.761999919132613 1101 64-65 0.7332931044202624 1101 66-67 0.7252865385907441 1101 68-69 0.7588856699505513 1101 70-71 0.7404434403250519 1101 72-73 0.7180419640240743 1101 74-75 0.691101640914674 1101 76-77 0.6972716428400858 1101 78-79 0.6693908224044804 1101 80-81 0.6729880555984522 1101 82-83 0.6706704411783733 1101 84-85 0.618402597739788 1101 86-87 0.5948467315875305 1101 88-89 0.6198075105156562 1101 90-91 0.6523106835361716 1101 92-93 0.6015818183215274 1101 94-95 0.6247762911215773 1101 96-97 0.6022930798604627 1101 98-99 0.6444324643675543 1101 100-101 0.7581039125005695 1102 1 0.4584989354012379 1102 2 0.4383007043993139 1102 3 0.4708157391839407 1102 4 0.41735662811059626 1102 5 0.4185317427019868 1102 6 0.4090512378402593 1102 7 0.42697588522224095 1102 8 0.4231585738026098 1102 9 0.4907243763923077 1102 10-11 0.5528446286739168 1102 12-13 0.5368514625259806 1102 14-15 0.6526421849577488 1102 16-17 0.6355620510057989 1102 18-19 0.6495881624421145 1102 20-21 0.6512665984581147 1102 22-23 0.6533657239921098 1102 24-25 0.6622179579358445 1102 26-27 0.6503041038884021 1102 28-29 0.6542959306650928 1102 30-31 0.7106250569613266 1102 32-33 0.6658188323464103 1102 34-35 0.6954154619779089 1102 36-37 0.6951690311122221 1102 38-39 0.6764800522708185 1102 40-41 0.6682660486519367 1102 42-43 0.6875296368408357 1102 44-45 0.6807453843573299 1102 46-47 0.6783686580188331 1102 48-49 0.6322024203903638 1102 50-51 0.5917664315322497 1102 52-53 0.6465785101241011 1102 54-55 0.7000014565504173 1102 56-57 0.7050784581327605 1102 58-59 0.6840943390724519 1102 60-61 0.7077703533644453 1102 62-63 0.7142878486698265 1102 64-65 0.7229406695269773 1102 66-67 0.7050613781300257 1102 68-69 0.741643275454102 1102 70-71 0.7118709252998556 1102 72-73 0.7079408900725284 1102 74-75 0.7032035678546649 1102 76-77 0.6992891707391422 1102 78-79 0.6907000817721922 1102 80-81 0.6729636002089805 1102 82-83 0.6740415190128957 1102 84-85 0.6739045121407088 1102 86-87 0.645507421907638 1102 88-89 0.629360013867192 1102 90-91 0.6451335034747743 1102 92-93 0.6644869899099533 1102 94-95 0.6824522357058171 1102 96-97 0.6698111256506927 1102 98-99 0.6962352162686152 1102 100-101 0.7512736155267667 1103 1 0.3805308216035712 1103 2 0.39375830109883836 1103 3 0.3936336451052824 1103 4 0.349771943270234 1103 5 0.3395885389068738 1103 6 0.3380328109824049 1103 7 0.3697292891841144 1103 8 0.3756551259540686 1103 9 0.4600795591903406 1103 10-11 0.4732921067571425 1103 12-13 0.4465369893514719 1103 14-15 0.5237224708883517 1103 16-17 0.5469830338116779 1103 18-19 0.5581170225730858 1103 20-21 0.5105820165594395 1103 22-23 0.5402437006314074 1103 24-25 0.5397753786502619 1103 26-27 0.5479644939982506 1103 28-29 0.5749683586625522 1103 30-31 0.5831966084193567 1103 32-33 0.5732405222805355 1103 34-35 0.6077406665601757 1103 36-37 0.5655971593876572 1103 38-39 0.5911818704517415 1103 40-41 0.5896243384990072 1103 42-43 0.5851782958499996 1103 44-45 0.5989198755368008 1103 46-47 0.5386515674561778 1103 48-49 0.5605870173485741 1103 50-51 0.5450759443696 1103 52-53 0.5649293890807243 1103 54-55 0.5371479301055615 1103 56-57 0.5602426664605176 1103 58-59 0.5592345528594436 1103 60-61 0.5771053412884939 1103 62-63 0.5815575087268599 1103 64-65 0.5954535900949551 1103 66-67 0.5981450715297925 1103 68-69 0.5839518654626872 1103 70-71 0.5873704175352188 1103 72-73 0.5694637778932403 1103 74-75 0.5922595704948961 1103 76-77 0.576933914881586 1103 78-79 0.5921135673662334 1103 80-81 0.5626486973232616 1103 82-83 0.5614632901994767 1103 84-85 0.5712269503222132 1103 86-87 0.5637234533197955 1103 88-89 0.5821978916324468 1103 90-91 0.5542603754436684 1103 92-93 0.5709207712619389 1103 94-95 0.5631603693037732 1103 96-97 0.5903781176354492 1103 98-99 0.612256231842526 1103 100-101 0.6914563793351114 1104 1 0.34807381765462253 1104 2 0.3670249276947821 1104 3 0.33755501035253843 1104 4 0.3193100214701161 1104 5 0.32451051476942894 1104 6 0.34360361566465514 1104 7 0.3752258505936794 1104 8 0.34949235001990786 1104 9 0.4525490921074251 1104 10-11 0.4353643284880846 1104 12-13 0.42352996340537885 1104 14-15 0.5317353884386549 1104 16-17 0.5024398569140303 1104 18-19 0.5103992298942543 1104 20-21 0.5328304567696662 1104 22-23 0.5369310527608491 1104 24-25 0.5604301164484937 1104 26-27 0.5419268345140509 1104 28-29 0.556502229169233 1104 30-31 0.562281956607741 1104 32-33 0.5530132626815956 1104 34-35 0.5794149556669765 1104 36-37 0.5726568773730989 1104 38-39 0.5890545855715885 1104 40-41 0.5797963294709731 1104 42-43 0.5655532274197341 1104 44-45 0.5850883558248015 1104 46-47 0.5552260446738089 1104 48-49 0.5819385511092605 1104 50-51 0.5756396197469726 1104 52-53 0.5999126732970268 1104 54-55 0.6144010624371674 1104 56-57 0.6352022791040568 1104 58-59 0.6253691288048984 1104 60-61 0.6308155803818636 1104 62-63 0.6333363677586163 1104 64-65 0.6453286405068184 1104 66-67 0.6129944540270316 1104 68-69 0.654115247240604 1104 70-71 0.6266211216918123 1104 72-73 0.6280311120373199 1104 74-75 0.6270278509252094 1104 76-77 0.6215720392255264 1104 78-79 0.6531980097804322 1104 80-81 0.6345596296461622 1104 82-83 0.6296884658796884 1104 84-85 0.6082337980776273 1104 86-87 0.6062423714207412 1104 88-89 0.6099669784535067 1104 90-91 0.6152946341941217 1104 92-93 0.6385586277454607 1104 94-95 0.6436149722737952 1104 96-97 0.6777793007039428 1104 98-99 0.7106079279664712 1104 100-101 0.7688833317490626 1105 1 0.3189339435339633 1105 2 0.32414471344229057 1105 3 0.3172334175810434 1105 4 0.316281645890065 1105 5 0.3069735401894391 1105 6 0.3245502584390536 1105 7 0.3294323822322127 1105 8 0.37225056019462244 1105 9 0.39640483684685535 1105 10-11 0.4083913235038281 1105 12-13 0.39370362031915107 1105 14-15 0.503877694734463 1105 16-17 0.4912028553041381 1105 18-19 0.4576842970433148 1105 20-21 0.48545673197185835 1105 22-23 0.5066235658627818 1105 24-25 0.509434178357246 1105 26-27 0.4813816892230065 1105 28-29 0.4896649142345879 1105 30-31 0.5314633630625281 1105 32-33 0.529829067773818 1105 34-35 0.5294450956684003 1105 36-37 0.4908416903293613 1105 38-39 0.5188836700440405 1105 40-41 0.5345521666332829 1105 42-43 0.5462239569042424 1105 44-45 0.5522779153742405 1105 46-47 0.5749717325939656 1105 48-49 0.5903928042565596 1105 50-51 0.5487550649660591 1105 52-53 0.561382842271172 1105 54-55 0.5845270451291427 1105 56-57 0.5972631208530643 1105 58-59 0.5626044890247286 1105 60-61 0.5970876950926893 1105 62-63 0.6015800078123661 1105 64-65 0.5850520563622084 1105 66-67 0.5708050912930531 1105 68-69 0.5865027235151743 1105 70-71 0.599472838784628 1105 72-73 0.5836707669064936 1105 74-75 0.6059955958838614 1105 76-77 0.5917911945463956 1105 78-79 0.5876110253035449 1105 80-81 0.583599487414709 1105 82-83 0.5665832975697356 1105 84-85 0.5865841559727301 1105 86-87 0.5594823845654844 1105 88-89 0.5609864545849277 1105 90-91 0.5949316228470494 1105 92-93 0.5929432602253542 1105 94-95 0.6108309722381478 1105 96-97 0.6282055036197711 1105 98-99 0.6200681490264692 1105 100-101 0.6940377180641555 1106 1 0.28265949591126827 1106 2 0.30890272661473617 1106 3 0.2973427484031248 1106 4 0.28500777816640266 1106 5 0.2950950750657668 1106 6 0.27341910053876006 1106 7 0.291383965375168 1106 8 0.32271271694012427 1106 9 0.3564951946675663 1106 10-11 0.3662346720535581 1106 12-13 0.3517238437491059 1106 14-15 0.41044847582144683 1106 16-17 0.4243534136995848 1106 18-19 0.4143869719031912 1106 20-21 0.40484668828543846 1106 22-23 0.41596047260352265 1106 24-25 0.42571729899251665 1106 26-27 0.47327621145518606 1106 28-29 0.46051156807478577 1106 30-31 0.4796324135649357 1106 32-33 0.46412933506365306 1106 34-35 0.4781419893999299 1106 36-37 0.44698613578654545 1106 38-39 0.4959698179193808 1106 40-41 0.4815129686550108 1106 42-43 0.4706712042112571 1106 44-45 0.49091974850122 1106 46-47 0.49262417297476446 1106 48-49 0.5180269654934975 1106 50-51 0.4619313922062531 1106 52-53 0.4949767403430556 1106 54-55 0.5184470531353114 1106 56-57 0.5313731496990997 1106 58-59 0.5636486971327272 1106 60-61 0.5522497567133016 1106 62-63 0.5364226916078252 1106 64-65 0.533100208794373 1106 66-67 0.5247120102672653 1106 68-69 0.5180496542816613 1106 70-71 0.5561821356109959 1106 72-73 0.5143218335773057 1106 74-75 0.5331735870620165 1106 76-77 0.5651882841854317 1106 78-79 0.5448099908205819 1106 80-81 0.53608548150698 1106 82-83 0.5105099828199897 1106 84-85 0.5475023771720657 1106 86-87 0.5426056104616634 1106 88-89 0.5519508086455645 1106 90-91 0.5699516790210453 1106 92-93 0.5598915012398464 1106 94-95 0.5673247235913408 1106 96-97 0.5655294055649982 1106 98-99 0.6119219050675291 1106 100-101 0.6829014628688093 1107 1 0.21416387622189603 1107 2 0.19403993801890707 1107 3 0.21680630102404308 1107 4 0.18881317065299186 1107 5 0.2080065885325837 1107 6 0.22930948594057554 1107 7 0.24625929202358066 1107 8 0.2306780384877385 1107 9 0.29816107064835506 1107 10-11 0.2818465350593655 1107 12-13 0.29848628862186644 1107 14-15 0.32831835577785284 1107 16-17 0.35487741219939295 1107 18-19 0.34068617757751696 1107 20-21 0.31246940300862747 1107 22-23 0.35589554847274485 1107 24-25 0.35599042097647526 1107 26-27 0.365005407311898 1107 28-29 0.3731374727757881 1107 30-31 0.3740271343132662 1107 32-33 0.36907024919067766 1107 34-35 0.38456606824014017 1107 36-37 0.3846437912501841 1107 38-39 0.3836554819852509 1107 40-41 0.3640198359053528 1107 42-43 0.4239135397574145 1107 44-45 0.3975456023037012 1107 46-47 0.4133924072251318 1107 48-49 0.42043723030047175 1107 50-51 0.33046237952518 1107 52-53 0.41567966771458487 1107 54-55 0.4396107043727042 1107 56-57 0.4497959911637466 1107 58-59 0.47087863442028777 1107 60-61 0.47652543065328956 1107 62-63 0.4765298300551706 1107 64-65 0.42829995654447117 1107 66-67 0.4508850968917102 1107 68-69 0.4687520860885641 1107 70-71 0.4643036449451685 1107 72-73 0.45739409609083026 1107 74-75 0.48455757974034697 1107 76-77 0.4671639492148927 1107 78-79 0.4504194633417029 1107 80-81 0.4641313222421317 1107 82-83 0.46169422790548964 1107 84-85 0.4630132713083839 1107 86-87 0.4495694776924637 1107 88-89 0.4576246690517465 1107 90-91 0.4557190151686399 1107 92-93 0.47764072918941736 1107 94-95 0.5021230445126719 1107 96-97 0.5338170027858773 1107 98-99 0.5277585819680546 1107 100-101 0.5864213726694381 1108 1 0.09364984906722285 1108 2 0.1227877820363581 1108 3 0.11674078451495973 1108 4 0.14852934488387604 1108 5 0.1342624395707972 1108 6 0.17683140948936682 1108 7 0.13843389934473294 1108 8 0.14874448969039378 1108 9 0.19660896762049163 1108 10-11 0.2113446474079197 1108 12-13 0.20281847959789445 1108 14-15 0.2235624609548168 1108 16-17 0.21515193934231291 1108 18-19 0.20051046945837925 1108 20-21 0.1998551552200709 1108 22-23 0.22576533107865515 1108 24-25 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-1.367194212570972 2316 82-83 -1.4436948289926939 2316 84-85 -1.4256487573411043 2316 86-87 -1.4879830661489137 2316 88-89 -1.4705537729114795 2316 90-91 -1.477117144569938 2316 92-93 -1.508422475914358 2316 94-95 -1.5006648140779575 2316 96-97 -1.5041339995573928 2316 98-99 -1.4557300550819399 2316 100-101 -1.6250762564826609 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 353789.0 3 80661.0 4 79181.0 5 80283.0 6 89847.0 7 95076.0 8 98619.0 9 112253.0 10 131675.0 11 148509.0 12 152789.0 13 152245.0 14 150225.0 15 151988.0 16 158321.0 17 167379.0 18 174642.0 19 183657.0 20 196016.0 21 213884.0 22 243776.0 23 287918.0 24 355815.0 25 449030.0 26 569923.0 27 720272.0 28 909653.0 29 1143528.0 30 1446411.0 31 1850702.0 32 2406578.0 33 3230421.0 34 4607980.0 35 7059276.0 36 1.1735732E7 37 1.8589983E7 38 2.1155687E7 39 5957968.0 40 8440.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 24.411851077456937 29.52948996527275 23.929681573550067 22.128977383720247 2 23.545605724942774 28.204496441666127 26.358650200738044 21.89124763265305 3 24.25828831833715 26.23331792051608 25.86449799536122 23.64389576578555 4 24.8086392969182 26.477240466577822 25.76198063916122 22.952139597342754 5 24.843773197588035 27.46647521483191 25.606211434310755 22.083540153269297 6 24.739133456065048 26.694068438460167 24.32965420194698 24.23714390352781 7 23.825623778890037 26.4918474538477 26.286756466696602 23.39577230056566 8 23.52152257866731 26.68507306970232 26.240687110145483 23.552717241484896 9 23.48093686912059 26.51184187005929 26.183873989331254 23.82334727148887 10-11 23.78237675115918 26.875932113815743 26.103363928904617 23.238327206120463 12-13 23.793642565450714 26.343821568457198 26.644419191690922 23.218116674401166 14-15 23.691894776790946 26.27154015415112 26.741602001392007 23.294963067665932 16-17 23.730471806148014 26.229861560347583 26.702643553121547 23.337023080382853 18-19 23.835515063568472 26.061546282345088 26.59310207306163 23.50983658102481 20-21 23.889946271859113 26.02020787926686 26.56909280392266 23.520753044951366 22-23 23.96600717352048 26.10193644535977 26.470622111601205 23.46143426951855 24-25 24.047535532569636 26.03371644236958 26.36205110827638 23.556696916784407 26-27 24.067609457649887 26.041182466375634 26.35599724380099 23.53521083217349 28-29 24.116483432198407 26.106950838180758 26.30695068994107 23.469615039679766 30-31 24.19342642499164 26.04055200480645 26.214956685608765 23.55106488459315 32-33 24.1961791930038 26.04356681795048 26.22413819840309 23.53611579064263 34-35 24.231855838889555 26.100636592378972 26.17398997046404 23.49351759826743 36-37 24.277159615343205 26.029654056433117 26.121413494150296 23.571772834073386 38-39 24.260959776673193 26.017564801646515 26.158010492432947 23.563464929247342 40-41 24.301269771589524 26.069328806343922 26.121148502158793 23.50825291990776 42-43 24.35047133311827 26.004873621976877 26.047964408436357 23.59669063646849 44-45 24.342177832069684 25.9985024368573 26.08296982481338 23.57634990625964 46-47 24.362966386413504 26.057294908183188 26.04944043485669 23.530298270546616 48-49 24.394565895613155 25.987089764027193 26.005097044143312 23.613247296216343 50-51 24.377289277190535 25.991413044229727 26.044333967132506 23.586963711447233 52-53 24.384755304673476 26.041454422071542 26.021888307466035 23.55190196578895 54-55 24.443426960393346 25.96951524990663 25.97910079740919 23.60795699229083 56-57 24.412330380249475 25.984887776933974 26.000263586983287 23.602518255833267 58-59 24.422018584088352 26.032131127410928 25.9918000062144 23.55405028228632 60-61 24.446988945428856 25.994714588330154 25.94122333468652 23.617073131554466 62-63 24.422858405321733 25.991386036846365 25.982225015153066 23.60353054267884 64-65 24.45401005289517 26.04321342041781 25.953785556832514 23.548990969854504 66-67 24.485619011839212 25.986782223707134 25.908203492219332 23.61939527223432 68-69 24.47207289454129 25.989784779855484 25.93616734170705 23.601974983896174 70-71 24.481010456192116 26.046562696213865 25.92083343978674 23.55159340780728 72-73 24.514888729169463 26.007113877963683 25.874586429379605 23.60341096348725 74-75 24.504052501712334 26.003106543244357 25.909789622276776 23.583051332766537 76-77 24.50773603471838 26.06691501514984 25.886688715337986 23.5386602347938 78-79 24.5439080930154 26.009421255712205 25.851477114236253 23.595193537036145 80-81 24.519669146494678 26.035907144905106 25.87294791880323 23.571475789796988 82-83 24.530797500471273 26.081747231501083 25.85647987792532 23.53097539010232 84-85 24.560008573448595 26.03717441429181 25.809719646690453 23.593097365569147 86-87 24.54424019863835 26.045088416680272 25.84165262305755 23.56901876162383 88-89 24.56385270681583 26.082845036795067 25.8248580386821 23.528444217707005 90-91 24.59385807065262 26.03838683525333 25.79290282841248 23.574852265681574 92-93 24.563808972753822 26.055274917681555 25.82051453679137 23.56040157277325 94-95 24.577661402836956 26.100483409884106 25.78672526055935 23.535129926719588 96-97 24.604411508480943 26.065533189335778 25.756134561544364 23.57392074063892 98-99 24.587509157187544 26.072414310534647 25.775156567708173 23.564919964569636 100-101 24.64905484923513 26.11004126367536 25.72607690409992 23.51482698298959 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 111.0 1 120.0 2 143.5 3 209.0 4 295.5 5 393.5 6 563.0 7 757.0 8 991.5 9 1283.5 10 1598.5 11 2045.0 12 2570.5 13 3142.5 14 3859.0 15 4892.5 16 6592.5 17 9421.0 18 14261.5 19 22204.5 20 35163.0 21 55458.5 22 85713.0 23 128782.0 24 186812.0 25 262327.5 26 355989.5 27 468193.5 28 600870.0 29 751818.0 30 915080.0 31 1097139.0 32 1298953.5 33 1510608.0 34 1727033.5 35 1939214.5 36 2144330.0 37 2344043.5 38 2516042.5 39 2646452.5 40 2734926.0 41 2776644.5 42 2772788.0 43 2737375.5 44 2686921.5 45 2631218.5 46 2580318.0 47 2540546.5 48 2510596.0 49 2488904.5 50 2468524.5 51 2432968.5 52 2383168.0 53 2327366.0 54 2268908.0 55 2206882.5 56 2146290.5 57 2086055.0 58 2017706.5 59 1941923.0 60 1858860.0 61 1763847.0 62 1658545.5 63 1546182.0 64 1422905.5 65 1283657.5 66 1135833.5 67 987590.5 68 838630.5 69 695025.0 70 566007.5 71 453735.0 72 358348.0 73 281296.5 74 220492.0 75 173625.0 76 136885.5 77 107805.5 78 84125.5 79 64449.0 80 48585.5 81 36353.0 82 27304.0 83 19923.5 84 13892.0 85 9397.0 86 6443.0 87 4492.5 88 3127.5 89 2130.5 90 1529.0 91 1122.5 92 737.5 93 531.0 94 416.0 95 298.5 96 213.0 97 155.5 98 96.5 99 51.0 100 23.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019820272855589065 2 0.002191361852278127 3 0.016186672851332365 4 0.011357042017158153 5 0.011260192691418492 6 0.004348884783514685 7 0.004691941431315415 8 0.0032438689826055343 9 0.0021015136826160316 10-11 0.00754491253292352 12-13 0.023508131819446904 14-15 0.0704053758050221 16-17 0.017965549924707235 18-19 0.006588671298662644 20-21 0.012352373039518772 22-23 0.009052494808292712 24-25 0.0015075822753691907 26-27 0.01804314607123359 28-29 0.03275199156052642 30-31 0.028462616603671043 32-33 0.03645326940686625 34-35 0.0468342335808771 36-37 0.023473709468732205 38-39 0.005103259350872412 40-41 0.005736863975892126 42-43 0.005039082086828058 44-45 0.011967309455252647 46-47 0.011795781131352283 48-49 0.02114290792457589 50-51 0.08224491416185917 52-53 0.09056462130069998 54-55 0.07093104594051267 56-57 0.0727741002779319 58-59 0.07037445403234618 60-61 0.04161020428766667 62-63 0.025572306002982586 64-65 0.022569393475379944 66-67 0.034267741851319436 68-69 0.06793280085029507 70-71 0.07019825827106077 72-73 0.07369358544278555 74-75 0.07218016887068505 76-77 0.07359906983573841 78-79 0.06673968716874322 80-81 0.022392030854748272 82-83 0.004361136806650426 84-85 0.014382708302012884 86-87 0.010177347218088299 88-89 0.005064752992445799 90-91 0.002670357613918261 92-93 0.0056942736097536 94-95 0.010840123326764537 96-97 0.04545733955228914 98-99 0.06016793533060136 100-101 0.08659963324210515 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8.5700132E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.43443429452087 #Duplication Level Percentage of deduplicated Percentage of total 1 70.01090375147729 47.91156592680496 2 19.679648487862647 26.935312227638065 3 6.592713119429834 13.535057783827401 4 2.419763872408069 6.623806869382615 5 0.8080347754519922 2.764870137417864 6 0.29952820800791546 1.2298826082163963 7 0.11682450972799827 0.5596373464479218 8 0.04197557426709309 0.22980597433249306 9 0.016791873730211963 0.10342281415248733 >10 0.013791423322289921 0.10560285258910454 >50 2.4404314670780357E-5 0.0010354591906810045 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.3337186925219674E-6 4.667437385043935E-6 1.1668593462609837E-6 5.834296731304918E-6 1.1668593462609837E-6 2 4.667437385043935E-6 1.1668593462609836E-5 1.1668593462609837E-6 1.283545280887082E-5 2.3337186925219674E-6 3 7.001156077565902E-6 1.866974954017574E-5 1.1668593462609837E-6 1.866974954017574E-5 5.834296731304918E-6 4 7.001156077565902E-6 2.567090561774164E-5 3.500578038782951E-6 3.267206169530754E-5 8.168015423826886E-6 5 9.33487477008787E-6 3.3838921041568524E-5 3.500578038782951E-6 3.967321777287344E-5 1.0501734116348852E-5 6 9.33487477008787E-6 3.733949908035148E-5 4.667437385043935E-6 5.0174951889222295E-5 1.4002312155131803E-5 7 1.4002312155131803E-5 4.0840077119134424E-5 1.283545280887082E-5 5.950982665931016E-5 1.633603084765377E-5 8 1.633603084765377E-5 4.0840077119134424E-5 1.283545280887082E-5 6.651098273687606E-5 1.866974954017574E-5 9 2.4504046271480657E-5 4.7841233196700324E-5 1.5169171501392787E-5 7.001156077565901E-5 1.866974954017574E-5 10-11 2.4504046271480657E-5 5.834296731304918E-5 1.633603084765377E-5 8.57641619501823E-5 2.4504046271480653E-5 12-13 2.917148365652459E-5 6.826127175626754E-5 1.633603084765377E-5 1.0443391149035804E-4 3.208863202217705E-5 14-15 3.5005780387829507E-5 7.934643554574688E-5 1.6919460520784262E-5 1.3068824678123017E-4 3.675606940722098E-5 16-17 3.967321777287344E-5 9.568246639340066E-5 1.983660888643672E-5 1.5402543370644984E-4 3.850635842661246E-5 18-19 4.7257803523569837E-5 1.0443391149035804E-4 2.6837764964002624E-5 1.8319691736297443E-4 4.25903661385259E-5 20-21 5.3092100254874754E-5 1.1201849724105443E-4 3.0338343002785574E-5 2.0186666690315014E-4 4.3173795811656396E-5 22-23 6.359383437122361E-5 1.1901965331862033E-4 3.150520234904656E-5 2.141186900388905E-4 4.434065515791738E-5 24-25 7.759614652635541E-5 1.301048171080997E-4 3.325549136843804E-5 2.3512215827158818E-4 5.0174951889222295E-5 26-27 8.926473998896524E-5 1.3827283253192656E-4 3.675606940722098E-5 2.5729248585054686E-4 5.484238927426623E-5 28-29 1.0385048181722753E-4 1.4760770730201444E-4 4.3173795811656396E-5 2.7771252441011413E-4 6.0093256332440654E-5 30-31 1.2602080939618624E-4 1.5752601174523278E-4 4.375722548478688E-5 2.969657036234203E-4 6.651098273687607E-5 32-33 1.5169171501392786E-4 1.6161001945714623E-4 4.7257803523569837E-5 3.103845861054216E-4 7.059499044878951E-5 34-35 1.773626206316695E-4 1.709448942272341E-4 5.075838156235279E-5 3.2438689826055347E-4 7.642928718009443E-5 36-37 1.9603237017184523E-4 1.8786435474801836E-4 5.250867058174426E-5 3.4364007747385967E-4 7.934643554574688E-5 38-39 2.14702119712021E-4 1.989495185374977E-4 5.250867058174426E-5 3.611429676677744E-4 8.401387293079083E-5 40-41 2.351221582715882E-4 2.0886782298071608E-4 5.4842389274266224E-5 3.7281156113038426E-4 8.51807322770518E-5 42-43 2.8062967277576653E-4 2.1878612742393442E-4 5.950982665931016E-5 3.86813873285516E-4 8.868131031583476E-5 44-45 3.2555375760681443E-4 2.3162158023280524E-4 6.067668600557115E-5 4.043167634794308E-4 9.159845868148721E-5 46-47 3.7339499080351474E-4 2.4387360336854558E-4 6.709441241000655E-5 4.218196536733455E-4 9.568246639340066E-5 48-49 4.2707052073151996E-4 2.555421968311554E-4 7.117842012192E-5 4.4690712961795674E-4 9.91830444321836E-5 50-51 4.964986518340485E-4 2.660439309475043E-4 7.292870914131147E-5 4.609094417730885E-4 1.0560077083661901E-4 52-53 5.770119467260564E-4 2.765456650638531E-4 7.467899816070296E-5 4.754951836013508E-4 1.1318535658731542E-4 54-55 6.639429680224997E-4 2.887976881995935E-4 7.467899816070296E-5 4.900809254296131E-4 1.2368709070366426E-4 56-57 7.520408486652039E-4 3.0280000035472524E-4 7.876300587261639E-5 5.122512530085718E-4 1.2952138743496918E-4 58-59 8.751445096957377E-4 3.1563545316359604E-4 8.168015423826885E-5 5.262535651637036E-4 1.365225435125351E-4 60-61 0.001021585357651491 3.2205317956803144E-4 8.226358391139934E-5 5.530913301277062E-4 1.4352369959010097E-4 62-63 0.0011843622364548982 3.302211949918584E-4 8.984816966209574E-5 5.676770719559685E-4 1.5169171501392786E-4 64-65 0.001380978036299874 3.348886323769023E-4 9.859961475905312E-5 5.799290950917089E-4 1.5927630076462428E-4 66-67 0.0015904292889537207 3.494743742051646E-4 1.0210019279783606E-4 5.898473995349272E-4 1.6627745684219016E-4 68-69 0.0018389703297073102 3.6055953799464394E-4 1.0326705214409705E-4 6.044331413631895E-4 1.709448942272341E-4 70-71 0.0021382697520232526 3.745618501497757E-4 1.0443391149035804E-4 6.254366095958873E-4 1.7386204259288657E-4 72-73 0.0024655737986494586 3.8739730295864655E-4 1.0618420050974951E-4 6.470235075017154E-4 1.849472063823659E-4 74-75 0.002887976881995934 4.008161854406479E-4 1.0910134887540197E-4 6.686104054075436E-4 1.983660888643672E-4 76-77 0.0034270658999685085 4.154019272689101E-4 1.1551907527983737E-4 6.849464362551974E-4 2.117849713463685E-4 78-79 0.004071172259104572 4.2415337236586755E-4 1.2018651266488131E-4 7.006990374297206E-4 2.1586897905828196E-4 80-81 0.004916561855470654 4.486574186373482E-4 1.2252023135740327E-4 7.112007715460695E-4 2.2345356480897837E-4 82-83 0.006028578812457372 4.5974258242682754E-4 1.2718766874244723E-4 7.298705210862452E-4 2.3103815055967473E-4 84-85 0.00753266050978778 4.690774571969154E-4 1.3243853580062164E-4 7.549579970308564E-4 2.4095645500289313E-4 86-87 0.009579915232802674 4.795791913132642E-4 1.353556841662741E-4 7.85296340033642E-4 2.5262504846550296E-4 88-89 0.01237220964840521 4.91247784775874E-4 1.3710597318566557E-4 8.016323708812958E-4 2.584593451968079E-4 >>END_MODULE >>Kmer Content pass >>END_MODULE