##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049542_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 59045363 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.750116634899847 33.0 31.0 34.0 30.0 34.0 2 31.899155857505693 34.0 31.0 34.0 30.0 34.0 3 31.93929431850559 34.0 31.0 34.0 30.0 34.0 4 35.43556460140655 37.0 35.0 37.0 33.0 37.0 5 35.42560437811179 37.0 35.0 37.0 33.0 37.0 6 35.451247204628075 37.0 35.0 37.0 33.0 37.0 7 35.44044735570514 37.0 35.0 37.0 33.0 37.0 8 35.42855521101632 37.0 35.0 37.0 33.0 37.0 9 37.07440706224467 39.0 37.0 39.0 33.0 39.0 10-11 37.04145113816982 39.0 37.0 39.0 33.0 39.0 12-13 37.00564637903911 39.0 37.0 39.0 33.0 39.0 14-15 38.294879514924816 40.0 38.0 41.0 33.0 41.0 16-17 38.29593148576291 40.0 38.0 41.0 33.0 41.0 18-19 38.28090865492689 40.0 38.0 41.0 33.5 41.0 20-21 38.24459598122887 40.0 38.0 41.0 33.0 41.0 22-23 38.17448625898024 40.0 38.0 41.0 33.0 41.0 24-25 38.11518563278204 40.0 38.0 41.0 33.0 41.0 26-27 38.02557755466759 40.0 38.0 41.0 33.0 41.0 28-29 37.91895555794957 40.0 38.0 41.0 33.0 41.0 30-31 37.80929408461762 40.0 38.0 41.0 33.0 41.0 32-33 37.69768434652523 40.0 38.0 41.0 32.0 41.0 34-35 37.578143477583495 40.0 37.0 41.0 32.0 41.0 36-37 37.464746401847 40.0 37.0 41.0 32.0 41.0 38-39 37.32171179132221 40.0 37.0 41.0 31.5 41.0 40-41 37.173505199722456 40.0 37.0 41.0 31.0 41.0 42-43 37.02166276799755 40.0 36.0 41.0 31.0 41.0 44-45 36.858591207238405 39.5 36.0 41.0 31.0 41.0 46-47 36.682559881967364 39.0 36.0 41.0 30.0 41.0 48-49 36.61570134643765 39.0 35.5 41.0 30.0 41.0 50-51 35.529153855824376 38.0 34.0 40.0 29.0 40.5 52-53 35.61525010863258 38.5 34.5 39.5 29.5 40.5 54-55 36.23237910316514 39.0 35.0 40.5 30.0 41.0 56-57 36.18434605101844 39.0 35.0 41.0 30.0 41.0 58-59 35.9824555316901 38.5 35.0 40.5 29.0 41.0 60-61 36.054696615210915 38.5 35.0 41.0 30.0 41.0 62-63 35.88723560730755 38.0 35.0 40.5 30.0 41.0 64-65 35.63452551727051 37.5 35.0 40.0 30.0 41.0 66-67 35.34763184536608 37.0 34.5 40.0 29.0 41.0 68-69 35.02573528932322 36.5 34.0 39.5 29.0 41.0 70-71 34.71156137188961 36.0 34.0 39.0 29.0 41.0 72-73 34.36931336166059 35.5 34.0 39.0 29.0 41.0 74-75 34.02843318111195 35.0 34.0 38.5 28.5 40.0 76-77 33.69188472090518 35.0 34.0 37.0 28.0 39.5 78-79 33.37439625868673 35.0 33.5 37.0 28.0 39.0 80-81 33.06552755040222 35.0 33.0 36.0 27.0 39.0 82-83 32.76473146587311 35.0 33.0 36.0 27.0 37.5 84-85 32.50158408713653 35.0 33.0 36.0 26.5 37.0 86-87 32.2681857845467 35.0 33.0 35.0 26.0 37.0 88-89 32.05789551162553 35.0 33.0 35.0 26.0 36.0 90-91 31.86824645315501 35.0 33.0 35.0 26.0 36.0 92-93 31.677297597442156 35.0 33.0 35.0 25.0 36.0 94-95 31.484411925793395 35.0 33.0 35.0 25.0 35.5 96-97 31.278317384550586 35.0 32.5 35.0 24.5 35.0 98-99 31.038457592343025 35.0 32.0 35.0 23.5 35.0 100-101 30.25638528973054 34.5 31.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5161590111267031 1101 2 0.5447426534931274 1101 3 0.5380412589418633 1101 4 0.46428502365782975 1101 5 0.48655882031146547 1101 6 0.500748218496561 1101 7 0.4754012305428219 1101 8 0.5065564616198941 1101 9 0.569931637783661 1101 10-11 0.6035740060963946 1101 12-13 0.6218386887678236 1101 14-15 0.740751265260954 1101 16-17 0.7284331797284622 1101 18-19 0.7163187551103789 1101 20-21 0.721528217775635 1101 22-23 0.7276494063893182 1101 24-25 0.7531002647924723 1101 26-27 0.7486803582815398 1101 28-29 0.7159855288423032 1101 30-31 0.7581891221905224 1101 32-33 0.7447816009157506 1101 34-35 0.7804749000431386 1101 36-37 0.7576076165832504 1101 38-39 0.7732481279734671 1101 40-41 0.7900029286873149 1101 42-43 0.7906519321680179 1101 44-45 0.778342013950386 1101 46-47 0.8283433875356252 1101 48-49 0.7484934406788781 1101 50-51 0.6966455066111834 1101 52-53 0.7280026113933644 1101 54-55 0.7526464506751225 1101 56-57 0.7365987303120605 1101 58-59 0.7118759467438949 1101 60-61 0.7618344074584442 1101 62-63 0.7846857023732028 1101 64-65 0.793910590648423 1101 66-67 0.7443663940437446 1101 68-69 0.7666448449202576 1101 70-71 0.7671588193307741 1101 72-73 0.7539340347212757 1101 74-75 0.7340844983380777 1101 76-77 0.7763075535448465 1101 78-79 0.7522369806491085 1101 80-81 0.7451941222255343 1101 82-83 0.7521685190894516 1101 84-85 0.6803319546459434 1101 86-87 0.6897560151740976 1101 88-89 0.7021113243229209 1101 90-91 0.7403425313402572 1101 92-93 0.6954053538478782 1101 94-95 0.6902451457070349 1101 96-97 0.7412003044193689 1101 98-99 0.757502229524615 1101 100-101 0.8535078922894499 1102 1 0.464432047136782 1102 2 0.46633762496820097 1102 3 0.4882349371499366 1102 4 0.42793539478516607 1102 5 0.43549032979807833 1102 6 0.41854549383334216 1102 7 0.4289656170332208 1102 8 0.4518651319326068 1102 9 0.5074948337962866 1102 10-11 0.5730802654284091 1102 12-13 0.5558781829265129 1102 14-15 0.6989157669126556 1102 16-17 0.6881835621943111 1102 18-19 0.6932054330054527 1102 20-21 0.683870064136876 1102 22-23 0.6839622324074597 1102 24-25 0.6927269721879767 1102 26-27 0.6894495721728617 1102 28-29 0.6741810929072969 1102 30-31 0.7525786307295661 1102 32-33 0.6930282856916534 1102 34-35 0.7088487270881743 1102 36-37 0.723875267352966 1102 38-39 0.7052232540313312 1102 40-41 0.7016988601243881 1102 42-43 0.6812972264873025 1102 44-45 0.7124943262880805 1102 46-47 0.7200203435782768 1102 48-49 0.6620825152993604 1102 50-51 0.6005217560585407 1102 52-53 0.6805372698556198 1102 54-55 0.7288286578362246 1102 56-57 0.74893832705758 1102 58-59 0.7199041207278611 1102 60-61 0.7345736712845365 1102 62-63 0.7407767318721241 1102 64-65 0.7460943439960914 1102 66-67 0.7326689012435992 1102 68-69 0.780817555641498 1102 70-71 0.7556366107765484 1102 72-73 0.7661525360933155 1102 74-75 0.7674695420236475 1102 76-77 0.7340766058280153 1102 78-79 0.7287901445268901 1102 80-81 0.7226473903663191 1102 82-83 0.7279356504065717 1102 84-85 0.7076764827914062 1102 86-87 0.6905587769766441 1102 88-89 0.6923935343107601 1102 90-91 0.7071064332878585 1102 92-93 0.7280845532186255 1102 94-95 0.743932055023695 1102 96-97 0.7639867680018106 1102 98-99 0.7999500295468849 1102 100-101 0.862454878879884 1103 1 0.3906756372486484 1103 2 0.38958611743340654 1103 3 0.4003930199202905 1103 4 0.35480056858722975 1103 5 0.3467126951885078 1103 6 0.3462252947066986 1103 7 0.3703318865313179 1103 8 0.373107373806441 1103 9 0.45114547147506556 1103 10-11 0.4736148294746769 1103 12-13 0.42963015793572623 1103 14-15 0.5230328044555748 1103 16-17 0.5556826314691037 1103 18-19 0.5511913945298801 1103 20-21 0.514124668231652 1103 22-23 0.5319914812201034 1103 24-25 0.5352097884825682 1103 26-27 0.5530655972167224 1103 28-29 0.5813244464793925 1103 30-31 0.5923281111640293 1103 32-33 0.5782691156497819 1103 34-35 0.5868649151742602 1103 36-37 0.552809713205086 1103 38-39 0.5937207317247655 1103 40-41 0.6033805128907588 1103 42-43 0.601122027998457 1103 44-45 0.6018094095860462 1103 46-47 0.5316257822099146 1103 48-49 0.5654325232814372 1103 50-51 0.5270616542775599 1103 52-53 0.5373639554160121 1103 54-55 0.530732767737625 1103 56-57 0.5456920333274198 1103 58-59 0.5667314487042532 1103 60-61 0.5823656129602739 1103 62-63 0.5871405388985593 1103 64-65 0.5943472753999828 1103 66-67 0.5918511166259037 1103 68-69 0.5839511707326182 1103 70-71 0.5854392494670577 1103 72-73 0.5725215179669689 1103 74-75 0.5891547897863347 1103 76-77 0.5590359206375766 1103 78-79 0.5667362742514044 1103 80-81 0.5560595221135145 1103 82-83 0.5321997845027155 1103 84-85 0.5594669448789773 1103 86-87 0.5566568659165867 1103 88-89 0.543872915793763 1103 90-91 0.5388645299859824 1103 92-93 0.5423809684974721 1103 94-95 0.5376975439760123 1103 96-97 0.5240320814484853 1103 98-99 0.5385913916839904 1103 100-101 0.6464793605099111 1104 1 0.35512969517387205 1104 2 0.3616554260839955 1104 3 0.3290316695954907 1104 4 0.3117995210765372 1104 5 0.3227923970236617 1104 6 0.3512529981345409 1104 7 0.39507985577775173 1104 8 0.36014522265716664 1104 9 0.46702222371560964 1104 10-11 0.42598090585082815 1104 12-13 0.4144599162986182 1104 14-15 0.506173063473895 1104 16-17 0.49176370781888323 1104 18-19 0.503809814293966 1104 20-21 0.5303488993334255 1104 22-23 0.5167243946074294 1104 24-25 0.550019990354734 1104 26-27 0.5334544482995724 1104 28-29 0.5334565907561455 1104 30-31 0.5708550675019737 1104 32-33 0.5336124231158053 1104 34-35 0.563424093018007 1104 36-37 0.5659051293211945 1104 38-39 0.5520474657390153 1104 40-41 0.5777449609390786 1104 42-43 0.5668670392053272 1104 44-45 0.5789529105504982 1104 46-47 0.5400752374659916 1104 48-49 0.5886816043871264 1104 50-51 0.5501880693871968 1104 52-53 0.5855882015672975 1104 54-55 0.6127477841370919 1104 56-57 0.6248126691521136 1104 58-59 0.6090479246962559 1104 60-61 0.6090188179622871 1104 62-63 0.6123565043669998 1104 64-65 0.6346520315497983 1104 66-67 0.6132527215646633 1104 68-69 0.6402491666593519 1104 70-71 0.6263445061140374 1104 72-73 0.6293640983108943 1104 74-75 0.6308797635413441 1104 76-77 0.6120391212032175 1104 78-79 0.6197703860972865 1104 80-81 0.619035482437468 1104 82-83 0.6245750606869152 1104 84-85 0.6038909000386425 1104 86-87 0.6078591542788843 1104 88-89 0.5909341978013671 1104 90-91 0.587465399547515 1104 92-93 0.6296474095902944 1104 94-95 0.6288507164170234 1104 96-97 0.6457758905528443 1104 98-99 0.6778916523414402 1104 100-101 0.732694727981194 1105 1 0.31760886361814045 1105 2 0.3185792269718455 1105 3 0.31634680158967043 1105 4 0.33277379031333254 1105 5 0.30998512546507584 1105 6 0.3290461966026115 1105 7 0.32866262868626706 1105 8 0.36754141898829573 1105 9 0.4020251247132123 1105 10-11 0.4139690642893328 1105 12-13 0.4108099110897072 1105 14-15 0.4910793772593962 1105 16-17 0.4770183942912922 1105 18-19 0.44559569298387913 1105 20-21 0.4766792384484475 1105 22-23 0.4996294251926088 1105 24-25 0.49023019660352674 1105 26-27 0.46916945993817194 1105 28-29 0.4959294161522436 1105 30-31 0.5365816030360193 1105 32-33 0.5294668619464744 1105 34-35 0.551247509918106 1105 36-37 0.5015221048420031 1105 38-39 0.526905746007337 1105 40-41 0.5304205994568463 1105 42-43 0.5289866793502256 1105 44-45 0.5362016538781944 1105 46-47 0.5787907221153077 1105 48-49 0.5774874106584562 1105 50-51 0.5265220154671013 1105 52-53 0.5647169526838738 1105 54-55 0.5936038244823649 1105 56-57 0.6236075754226675 1105 58-59 0.58784437018533 1105 60-61 0.6118465377414708 1105 62-63 0.5962857920670572 1105 64-65 0.584633517693824 1105 66-67 0.5848962901933916 1105 68-69 0.5843322294259181 1105 70-71 0.6102316442548528 1105 72-73 0.6045235556696511 1105 74-75 0.6194973332041229 1105 76-77 0.6038707174684674 1105 78-79 0.5880380926367437 1105 80-81 0.6054710655022788 1105 82-83 0.6004419394510379 1105 84-85 0.6062615017460118 1105 86-87 0.5792416803674882 1105 88-89 0.5843792009560573 1105 90-91 0.6011395638785935 1105 92-93 0.6132945765371751 1105 94-95 0.6500124820157573 1105 96-97 0.6538471645942145 1105 98-99 0.6590417488520188 1105 100-101 0.7250208276558432 1106 1 0.297908572323351 1106 2 0.3074708661444312 1106 3 0.2916868070616978 1106 4 0.31279805526570925 1106 5 0.31902426283132 1106 6 0.316454059131857 1106 7 0.3283904400564026 1106 8 0.3469610642082017 1106 9 0.37514343665717576 1106 10-11 0.39897659473194125 1106 12-13 0.3811576126978409 1106 14-15 0.43413425395966243 1106 16-17 0.448514783134236 1106 18-19 0.43441726423254323 1106 20-21 0.4381381097961068 1106 22-23 0.4476786545616065 1106 24-25 0.4527275997150255 1106 26-27 0.4714587006058295 1106 28-29 0.49365895519424896 1106 30-31 0.5114138907690275 1106 32-33 0.48953123487387273 1106 34-35 0.4795272879780015 1106 36-37 0.460021560710409 1106 38-39 0.5018475249686247 1106 40-41 0.5107806694641539 1106 42-43 0.49670460809758765 1106 44-45 0.5222333784562352 1106 46-47 0.5112432634103712 1106 48-49 0.536830976791741 1106 50-51 0.4858490169231189 1106 52-53 0.5242429605823702 1106 54-55 0.5462997078493004 1106 56-57 0.5479015802391842 1106 58-59 0.6026520474262469 1106 60-61 0.5590379253803732 1106 62-63 0.559406946435665 1106 64-65 0.5568851584867573 1106 66-67 0.5495641266570814 1106 68-69 0.5634253563045277 1106 70-71 0.5625030738722927 1106 72-73 0.557041176325896 1106 74-75 0.5548333800567491 1106 76-77 0.5881571447111824 1106 78-79 0.5937024038209486 1106 80-81 0.5762255313093618 1106 82-83 0.5623940907722229 1106 84-85 0.5877974515484468 1106 86-87 0.6185803395163703 1106 88-89 0.6064331430968934 1106 90-91 0.602645725834396 1106 92-93 0.5927198748295446 1106 94-95 0.5922313335197096 1106 96-97 0.6111158174769287 1106 98-99 0.6701178621988717 1106 100-101 0.7359492481425356 1107 1 0.21215494058759177 1107 2 0.19886947362001806 1107 3 0.21900408282312966 1107 4 0.18369956346563043 1107 5 0.20290455425768528 1107 6 0.2220815306056778 1107 7 0.22791710485542893 1107 8 0.2180679602283888 1107 9 0.28597191443437 1107 10-11 0.2864634759546618 1107 12-13 0.2993690397624036 1107 14-15 0.3422295352217901 1107 16-17 0.3511942467621125 1107 18-19 0.3328147750877477 1107 20-21 0.30346121861461484 1107 22-23 0.3578981137892967 1107 24-25 0.37754083861317156 1107 26-27 0.4022335878503114 1107 28-29 0.40171036104963775 1107 30-31 0.3925985897848747 1107 32-33 0.3807412730507025 1107 34-35 0.3862255457415529 1107 36-37 0.39547156386732496 1107 38-39 0.4150051871329836 1107 40-41 0.3784007179896349 1107 42-43 0.442256951198317 1107 44-45 0.4436919467886469 1107 46-47 0.45217693322030783 1107 48-49 0.4558457996589098 1107 50-51 0.34855614514860633 1107 52-53 0.448216625308568 1107 54-55 0.46791465852547987 1107 56-57 0.4977726023559441 1107 58-59 0.5179963937723286 1107 60-61 0.502979728989736 1107 62-63 0.5125958722867665 1107 64-65 0.46240356617732914 1107 66-67 0.491728957416818 1107 68-69 0.49588788484231117 1107 70-71 0.49805727359853336 1107 72-73 0.4905584027010903 1107 74-75 0.5055333982019192 1107 76-77 0.5045322709462496 1107 78-79 0.48731073595207164 1107 80-81 0.47823092201614514 1107 82-83 0.48161246662282764 1107 84-85 0.4865672814620652 1107 86-87 0.4673355797069476 1107 88-89 0.48587475498836596 1107 90-91 0.4901472456790863 1107 92-93 0.5135382749072086 1107 94-95 0.544359224259555 1107 96-97 0.5719471649569599 1107 98-99 0.5590209114581413 1107 100-101 0.6111087121810819 1108 1 0.07784665150652259 1108 2 0.10274075335563637 1108 3 0.09777251441010648 1108 4 0.11385872358906823 1108 5 0.13723215880914807 1108 6 0.15388299084732893 1108 7 0.11765370882258708 1108 8 0.1182670106027146 1108 9 0.16716414367116528 1108 10-11 0.20056666969099268 1108 12-13 0.17947435213931584 1108 14-15 0.20012158287592285 1108 16-17 0.19610455129404158 1108 18-19 0.17603445065029888 1108 20-21 0.1738307168381752 1108 22-23 0.19902702578405496 1108 24-25 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-1.489898157211961 2316 92-93 -1.516946393069869 2316 94-95 -1.52570376667094 2316 96-97 -1.5347481006397388 2316 98-99 -1.5088976726349372 2316 100-101 -1.666457187439054 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 290565.0 3 62575.0 4 60010.0 5 60387.0 6 67551.0 7 70083.0 8 73688.0 9 84027.0 10 98935.0 11 110548.0 12 113169.0 13 111598.0 14 110446.0 15 111946.0 16 116677.0 17 122059.0 18 127138.0 19 133253.0 20 141616.0 21 154876.0 22 175579.0 23 207274.0 24 255485.0 25 320666.0 26 405878.0 27 512272.0 28 641231.0 29 803177.0 30 1008143.0 31 1282086.0 32 1663689.0 33 2226873.0 34 3176411.0 35 4877754.0 36 8161450.0 37 1.2897371E7 38 1.430366E7 39 3895800.0 40 9417.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 25.918797976449092 30.299568795100125 22.484395849336916 21.297237379113866 2 24.078879898771245 28.690718116217283 25.70229549808303 21.528106486928447 3 24.91113105757336 26.02957425332184 25.49926053356429 23.56003415554051 4 25.403154673345824 26.455876665980842 25.318692937831877 22.822275722841454 5 25.42202381215526 27.78800694886916 24.90618394848353 21.883785290492057 6 25.20253918290728 26.768537785310322 23.35560526603315 24.673317765749246 7 24.050094895197788 26.477884923001994 25.907512762458595 23.564507419341624 8 23.716789596587567 26.631748959697372 25.75011606717202 23.90134537654304 9 23.73514135238785 26.415954266565777 25.682703637561016 24.16620074348536 10-11 24.083828892460286 26.885192182114043 25.616361914839857 23.414617010585815 12-13 24.026709301018528 26.205539211534685 26.368844181172683 23.3989073062741 14-15 23.892867894071422 26.100334181297175 26.466314546260477 23.540483378370926 16-17 23.94353833933497 26.072759314044326 26.407626649621285 23.576075696999414 18-19 24.039429803264674 25.899704938806455 26.282900777142594 23.777964480786277 20-21 24.07051975840219 25.870032548762566 26.286200698531037 23.773246994304206 22-23 24.143784802047648 25.970599785025694 26.18454174311438 23.701073669812278 24-25 24.22597938669533 25.881769542105136 26.090340413686885 23.801910657512646 26-27 24.22845879818952 25.92065807682909 26.08440197561544 23.76648114936595 28-29 24.264511251333232 25.988991815025166 26.054901223053356 23.691595710588246 30-31 24.370389878264483 25.910322340022613 25.94985250769946 23.769435274013446 32-33 24.347495724710114 25.929274624474463 25.97677182147994 23.746457829335487 34-35 24.391017857039678 25.969230661309627 25.92560712506519 23.7141443565855 36-37 24.42238095499054 25.89309500825574 25.874959732208097 23.80956430454562 38-39 24.395959272274716 25.87885794150926 25.929270073753013 23.795912712463014 40-41 24.45132516832597 25.94930081408522 25.875082353571038 23.72429166401777 42-43 24.498563714893844 25.863742109950522 25.812848891066086 23.824845284089545 44-45 24.48189358954625 25.861114775173927 25.84574250839129 23.81124912688853 46-47 24.50333915577074 25.92513965008057 25.815365950049085 23.75615524409961 48-49 24.538430156244008 25.83978609210237 25.76033605376641 23.86144769788722 50-51 24.504260375455107 25.855700108485674 25.813064360385173 23.82697515567404 52-53 24.50854826054306 25.90337836510912 25.802226958309028 23.785846416038794 54-55 24.578859358760386 25.81709446684591 25.74284073112643 23.861205443267277 56-57 24.520844493823308 25.847994348990994 25.77577627753298 23.855384879652718 58-59 24.536660422015576 25.882220834898607 25.775309011760044 23.805809731325773 60-61 24.57074383076732 25.841896916168132 25.7013863393967 23.88597291366785 62-63 24.531700378196188 25.842267881865272 25.768536823766443 23.857494916172097 64-65 24.55806375792739 25.89951361023176 25.727652221786958 23.81477041005389 66-67 24.592264105711408 25.835058454193444 25.676695493508245 23.895981946586904 68-69 24.560379279192937 25.83969491846858 25.72492581293088 23.8749999894076 70-71 24.590313898296134 25.898102144587448 25.687126595989586 23.82445736112684 72-73 24.616404959923983 25.866189996026606 25.64856301660984 23.868842027439566 74-75 24.60976500793487 25.853760889105754 25.684590092861782 23.85188401009759 76-77 24.605718926475404 25.92331465730931 25.669580871255686 23.8013855449596 78-79 24.643497653653917 25.853037729011813 25.6320942493328 23.87137036800147 80-81 24.614895245781142 25.89350028687835 25.647941057043816 23.84366341029669 82-83 24.632239009808785 25.93795826319436 25.656351155792674 23.773451571204184 84-85 24.66214740383734 25.889572340572105 25.572130330239794 23.876149925350756 86-87 24.63415243909214 25.90484237087729 25.619035502833498 23.841969687197068 88-89 24.655930870951497 25.934745202076137 25.6111276938306 23.79819623314176 90-91 24.680541866802667 25.891192080781916 25.56187135555567 23.86639469685975 92-93 24.644343592237206 25.913166158671473 25.601424841847702 23.841065407243615 94-95 24.667555781177082 25.945781043188493 25.578976210248673 23.80768696538576 96-97 24.698030687166277 25.915743899994652 25.52462506666067 23.861600346178403 98-99 24.6696215084172 25.92216493374772 25.56681387493959 23.841399682895485 100-101 24.726443155489935 25.9604110338379 25.50249468016757 23.8106511305046 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 66.0 1 73.5 2 100.5 3 134.0 4 196.0 5 278.0 6 349.5 7 462.0 8 651.0 9 848.5 10 1063.5 11 1319.5 12 1660.5 13 2079.5 14 2577.5 15 3183.5 16 4123.0 17 5678.0 18 8290.0 19 12789.0 20 20269.0 21 31746.0 22 48838.0 23 74509.0 24 109695.5 25 156144.5 26 215046.0 27 287135.5 28 372091.0 29 470177.5 30 579655.5 31 700259.5 32 833758.5 33 978169.5 34 1126773.5 35 1274046.0 36 1421669.5 37 1563774.0 38 1685238.0 39 1780090.5 40 1847341.5 41 1880914.5 42 1879958.0 43 1857383.5 44 1828095.5 45 1798416.0 46 1769864.0 47 1751882.5 48 1743547.0 49 1736405.0 50 1729715.5 51 1711968.5 52 1682521.5 53 1650333.5 54 1614956.5 55 1578356.5 56 1543987.0 57 1505127.5 58 1458458.0 59 1406160.0 60 1346250.5 61 1277214.0 62 1201463.0 63 1117247.5 64 1025995.5 65 925060.0 66 815856.5 67 707415.0 68 598392.0 69 492506.0 70 397539.0 71 316113.0 72 248131.5 73 192957.0 74 149615.5 75 116821.0 76 91093.5 77 70953.0 78 54829.5 79 41099.0 80 30535.0 81 22676.5 82 16705.5 83 12002.5 84 8303.0 85 5583.0 86 3784.5 87 2598.0 88 1794.0 89 1219.0 90 867.0 91 657.5 92 498.5 93 359.5 94 268.5 95 210.5 96 159.0 97 108.0 98 60.0 99 32.0 100 16.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019833902960339156 2 0.0021525822442653116 3 0.01617908590044573 4 0.011506407370211273 5 0.011333658834479517 6 0.004386457917110273 7 0.004843733452870804 8 0.0033313369586702346 9 0.0021932289585551365 10-11 0.007866832828176532 12-13 0.023865548933961162 14-15 0.0712350604060136 16-17 0.01827493210601483 18-19 0.006573759229831478 20-21 0.012409949956612173 22-23 0.008949898402690826 24-25 0.0015657453067059643 26-27 0.01828594059113499 28-29 0.03327441648550793 30-31 0.02883799698208308 32-33 0.03705625452755706 34-35 0.04726874149287557 36-37 0.02371651098156514 38-39 0.005128260452899578 40-41 0.005840424759519219 42-43 0.005277298405295603 44-45 0.012237201420880417 46-47 0.01200263600716622 48-49 0.021452150273002808 50-51 0.08314878172567082 52-53 0.09161176636343145 54-55 0.07159071915604956 56-57 0.07368995258780948 58-59 0.07124437527803835 60-61 0.042162498010216314 62-63 0.025889416582975364 64-65 0.022843453430881608 66-67 0.03474108542613245 68-69 0.06911296319746565 70-71 0.07126723905482636 72-73 0.07481789890935213 74-75 0.07309295397167767 76-77 0.07444699764145746 78-79 0.06774621742947029 80-81 0.022648687924909528 82-83 0.004173062667088692 84-85 0.014558298168138962 86-87 0.01019385722126901 88-89 0.005041039378485996 90-91 0.002631874750266164 92-93 0.005591463634494041 94-95 0.01096360437313257 96-97 0.04613148029930818 98-99 0.06115890929487553 100-101 0.0876080650058837 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5.9045363E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.72516291432143 #Duplication Level Percentage of deduplicated Percentage of total 1 74.84215350507989 55.17749960020668 2 17.96498123498202 26.489423366035535 3 5.01622967294208 11.094670495599244 4 1.4826345993488508 4.372299095176149 5 0.459992684684831 1.6956517808892628 6 0.1465637022667163 0.6483259696163858 7 0.050409758484231626 0.26015273597051014 8 0.019260563025657932 0.11359905175105493 9 0.006823670556152544 0.045276860108340415 >10 0.01095060862962554 0.10310104464687389 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.774452381637488E-6 1.016167857245623E-5 3.387226190818744E-6 3.387226190818744E-6 0.0 2 8.468065477046859E-6 1.3548904763274976E-5 5.080839286228115E-6 1.6936130954093718E-5 3.387226190818744E-6 3 1.016167857245623E-5 1.6936130954093718E-5 6.774452381637488E-6 2.540419643114058E-5 3.387226190818744E-6 4 1.1855291667865604E-5 1.862974404950309E-5 6.774452381637488E-6 3.3872261908187436E-5 8.468065477046859E-6 5 2.2016970240321835E-5 2.032335714491246E-5 8.468065477046859E-6 4.064671428982492E-5 1.016167857245623E-5 6 2.7097809526549952E-5 2.2016970240321835E-5 8.468065477046859E-6 4.9114779766871784E-5 1.6936130954093718E-5 7 2.7097809526549952E-5 2.371058333573121E-5 8.468065477046859E-6 5.4195619053099905E-5 2.032335714491246E-5 8 3.556587500359681E-5 2.8791422621959322E-5 1.016167857245623E-5 5.927645833932802E-5 2.2016970240321835E-5 9 3.556587500359681E-5 3.217864881277807E-5 1.3548904763274976E-5 7.282536310260299E-5 2.2016970240321835E-5 10-11 4.403394048064367E-5 3.979990774212024E-5 1.7782937501798405E-5 9.06083006044014E-5 2.6251002978845266E-5 12-13 4.657436012375773E-5 5.334881250539522E-5 2.032335714491246E-5 1.0585081846308574E-4 3.7259488099006176E-5 14-15 5.842965179162333E-5 6.0123264887032705E-5 2.2863776788026522E-5 1.2871459525111228E-4 5.080839286228116E-5 16-17 6.689771726867019E-5 7.282536310260299E-5 2.540419643114058E-5 1.4565072620520599E-4 5.1655199409985844E-5 18-19 7.451897619801237E-5 8.891468750899203E-5 2.6251002978845266E-5 1.6089324406389033E-4 5.504242560080459E-5 20-21 9.230191369981077E-5 1.0161678572456232E-4 2.7944616074254636E-5 1.8291021430421218E-4 5.842965179162333E-5 22-23 1.0669762501079044E-4 1.1093165774931386E-4 3.471906845589212E-5 2.0238676490141996E-4 6.351049107785145E-5 24-25 1.2363375596488417E-4 1.1855291667865604E-4 3.8953101194415557E-5 2.0916121728305746E-4 6.520410417326083E-5 26-27 1.3972308037127318E-4 1.2956140179881697E-4 3.979990774212024E-5 2.2440373514174178E-4 6.943813691178425E-5 28-29 1.6512727680241377E-4 1.4141669346668257E-4 4.23403273852343E-5 2.515015446682917E-4 7.451897619801237E-5 30-31 1.8968466668584967E-4 1.4734433930061537E-4 4.403394048064367E-5 2.752121280040229E-4 8.044662203194517E-5 32-33 2.1254844347387618E-4 1.5242517858684348E-4 5.080839286228116E-5 2.8791422621959325E-4 8.214023512735454E-5 34-35 2.3541222026190269E-4 1.566592113253669E-4 5.588923214850927E-5 3.014631309828682E-4 8.637426786587797E-5 36-37 2.9214825895811666E-4 1.6089324406389033E-4 5.673603869621396E-5 3.260205208663041E-4 8.637426786587797E-5 38-39 3.37029005986465E-4 1.6597408335011847E-4 6.181687798244209E-5 3.5311833039285406E-4 8.806788096128734E-5 40-41 3.9207143158726963E-4 1.7698256847027937E-4 6.520410417326082E-5 3.6497362206071966E-4 9.230191369981078E-5 42-43 4.3864579171102734E-4 1.9476550597207778E-4 7.536578274571705E-5 3.7090126789465246E-4 9.65359464383342E-5 44-45 5.030030893365834E-4 2.0916121728305743E-4 7.959981548424049E-5 3.9037781849186025E-4 9.992317262915294E-5 46-47 5.775220655345958E-4 2.2525054168944647E-4 8.129342857964985E-5 4.0307991670743054E-4 1.0331039881997169E-4 48-49 6.511942351849036E-4 2.404930595481308E-4 8.214023512735454E-5 4.1239478873218206E-4 1.0585081846308574E-4 50-51 7.392621161461908E-4 2.531951577637011E-4 8.38338482227639E-5 4.2848411313857113E-4 1.1177846429701854E-4 52-53 8.849128423513968E-4 2.6759086907468077E-4 8.976149405669671E-5 4.4203301790184606E-4 1.2109333632177009E-4 54-55 0.001008546598316281 2.769057410994323E-4 9.314872024751546E-5 4.6150956849905385E-4 1.2871459525111228E-4 56-57 0.001170286648927876 2.9722909824434477E-4 9.822955953374357E-5 4.7844569945314756E-4 1.329486279896357E-4 58-59 0.0013574308959706117 3.2093968158007603E-4 9.992317262915294E-5 4.911477976687179E-4 1.3548904763274974E-4 60-61 0.001575060178730716 3.2771413396171347E-4 9.992317262915294E-5 5.131647679090397E-4 1.4226350001438724E-4 62-63 0.00183418298232835 3.378758125341697E-4 1.0415720536767638E-4 5.292540923154288E-4 1.507315654914341E-4 64-65 0.0021331056936681036 3.4380345836810254E-4 1.0669762501079044E-4 5.487306429126365E-4 1.685145029932325E-4 66-67 0.0024633602472729317 3.5227152384514936E-4 1.0923804465390449E-4 5.631263542236161E-4 1.7359534227946063E-4 68-69 0.0028545848723124964 3.6751404170383374E-4 1.1008485120160918E-4 5.826029048208239E-4 1.7952298811339343E-4 70-71 0.003251737143185994 3.9376504468267897E-4 1.1008485120160918E-4 6.054666816088506E-4 1.922250863289637E-4 72-73 0.0037013914200171825 4.0223311015972585E-4 1.1093165774931386E-4 6.401857500647426E-4 2.0153995835371527E-4 74-75 0.0042831475182903015 4.1239478873218206E-4 1.1347207739242792E-4 6.664367530435878E-4 2.108548303784668E-4 76-77 0.004932648140379796 4.293309196862758E-4 1.1347207739242792E-4 6.842196905453863E-4 2.2355692859403708E-4 78-79 0.0057659057833212065 4.3949259825873204E-4 1.1347207739242792E-4 6.98615401856366E-4 2.3541222026190269E-4 80-81 0.006753282217944871 4.5388830956971166E-4 1.2109333632177009E-4 7.163983393581643E-4 2.3795263990501677E-4 82-83 0.008081074884745819 4.6404998814216793E-4 1.2871459525111228E-4 7.44342955432419E-4 2.4133986609583549E-4 84-85 0.009830577212303699 4.7251805361921476E-4 1.371826607281591E-4 7.604322798388081E-4 2.489611250251777E-4 86-87 0.012101712373247666 4.8267973219167097E-4 1.4056988691897788E-4 7.731343780543784E-4 2.5658238395451986E-4 88-89 0.015240824245588938 4.911477976687179E-4 1.4141669346668257E-4 7.934577351992908E-4 2.599696101453386E-4 >>END_MODULE >>Kmer Content pass >>END_MODULE