##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049541_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	64045590
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.525137015678986	33.0	31.0	34.0	30.0	34.0
2	31.664290734147347	34.0	31.0	34.0	30.0	34.0
3	31.7456475301422	34.0	31.0	34.0	30.0	34.0
4	35.199731066573044	37.0	35.0	37.0	33.0	37.0
5	35.17341848205317	37.0	35.0	37.0	33.0	37.0
6	35.16720490825364	37.0	35.0	37.0	32.0	37.0
7	35.148866815029734	37.0	35.0	37.0	32.0	37.0
8	35.19089066085581	37.0	35.0	37.0	33.0	37.0
9	36.772411090287406	39.0	37.0	39.0	33.0	39.0
10-11	36.79657488517164	39.0	37.0	39.0	33.0	39.0
12-13	36.75526184550724	39.0	37.0	39.0	33.0	39.0
14-15	37.995397200650345	40.0	38.0	41.0	33.0	41.0
16-17	38.017553097723045	40.0	38.0	41.0	33.0	41.0
18-19	38.06291924237094	40.0	38.0	41.0	33.0	41.0
20-21	38.07097113946487	40.0	38.0	41.0	33.0	41.0
22-23	38.01929335025253	40.0	38.0	41.0	33.0	41.0
24-25	37.944611970941324	40.0	38.0	41.0	33.0	41.0
26-27	37.874439941922624	40.0	38.0	41.0	33.0	41.0
28-29	37.7851329966669	40.0	38.0	41.0	32.0	41.0
30-31	37.71464345944818	40.0	38.0	41.0	32.0	41.0
32-33	37.622893785504985	40.0	38.0	41.0	32.0	41.0
34-35	37.55655033391058	40.0	38.0	41.0	32.0	41.0
36-37	37.48076250839441	40.0	38.0	41.0	32.0	41.0
38-39	37.39594477933609	40.0	37.0	41.0	31.5	41.0
40-41	37.294874362153585	40.0	37.0	41.0	31.0	41.0
42-43	37.19115996120888	40.0	37.0	41.0	31.0	41.0
44-45	37.0478771684358	40.0	37.0	41.0	31.0	41.0
46-47	36.90348342485407	40.0	36.0	41.0	31.0	41.0
48-49	36.75796640330739	40.0	36.0	41.0	30.0	41.0
50-51	36.61418237383714	40.0	36.0	41.0	30.0	41.0
52-53	36.450756835872696	39.5	35.0	41.0	30.0	41.0
54-55	36.255757781292985	39.0	35.0	41.0	30.0	41.0
56-57	36.03115461970137	39.0	35.0	41.0	29.0	41.0
58-59	35.818517574746366	39.0	35.0	41.0	29.0	41.0
60-61	35.58135607775648	38.0	35.0	41.0	28.5	41.0
62-63	35.324579873493235	38.0	34.5	40.0	28.0	41.0
64-65	35.112129219201506	37.0	34.0	40.0	28.0	41.0
66-67	34.87701727004154	37.0	34.0	40.0	28.0	41.0
68-69	34.600067592475924	36.5	34.0	39.5	28.0	41.0
70-71	34.282329868457765	36.0	34.0	39.0	28.0	41.0
72-73	33.956516693811395	35.5	34.0	39.0	27.0	41.0
74-75	33.63097032129769	35.0	34.0	38.5	27.0	40.0
76-77	33.28482263181587	35.0	33.0	37.0	26.0	39.5
78-79	33.06590108702254	35.0	33.0	37.0	26.0	39.0
80-81	32.78912430972999	35.0	33.0	36.5	26.0	39.0
82-83	32.51601467017479	35.0	33.0	36.0	26.0	38.0
84-85	32.23822111717607	35.0	33.0	36.0	26.0	37.0
86-87	32.023739597058906	35.0	33.0	35.0	25.5	37.0
88-89	31.821072723352223	35.0	33.0	35.0	25.0	36.0
90-91	31.627862878615062	35.0	33.0	35.0	25.0	36.0
92-93	31.452656904245867	35.0	33.0	35.0	24.5	36.0
94-95	31.277134475613387	35.0	33.0	35.0	24.0	36.0
96-97	31.124448716921805	35.0	33.0	35.0	23.5	35.0
98-99	30.96247859532561	35.0	33.0	35.0	23.0	35.0
100	30.81881762663128	35.0	32.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3426797664569925
1101	2	0.33146327650432283
1101	3	0.2585745755420703
1101	4	0.2763908899364296
1101	5	0.24839204672420578
1101	6	0.21215218242732448
1101	7	0.25512674494088117
1101	8	0.25525860725024074
1101	9	0.32359465337086846
1101	10-11	0.2804681979208894
1101	12-13	0.2743891989415488
1101	14-15	0.4048473914661983
1101	16-17	0.2995048045460109
1101	18-19	0.304117544462315
1101	20-21	0.3171454175339008
1101	22-23	0.29456190822619277
1101	24-25	0.35306618340538165
1101	26-27	0.31052585764761886
1101	28-29	0.35402980874088996
1101	30-31	0.32730641858114495
1101	32-33	0.38679449283356604
1101	34-35	0.3790101769620904
1101	36-37	0.3882475161753618
1101	38-39	0.389168098491389
1101	40-41	0.32342121791222667
1101	42-43	0.3101680710018968
1101	44-45	0.30060295886998034
1101	46-47	0.2644673092180554
1101	48-49	0.2859912973261842
1101	50-51	0.24770802211001808
1101	52-53	0.18079619760771237
1101	54-55	0.20962264086423943
1101	56-57	0.24243945678718148
1101	58-59	0.2807236686221444
1101	60-61	0.220980730637379
1101	62-63	0.21937120467676152
1101	64-65	0.2774741600724724
1101	66-67	0.30155223763259187
1101	68-69	0.27663166666221173
1101	70-71	0.24072416376087347
1101	72-73	0.1322804086059648
1101	74-75	0.17866745654963267
1101	76-77	0.1545625907730468
1101	78-79	0.2147443120617396
1101	80-81	0.18775101186803766
1101	82-83	0.21335993525443797
1101	84-85	0.19032198673670564
1101	86-87	0.17652788793789398
1101	88-89	0.13519757920454722
1101	90-91	0.18647336559212846
1101	92-93	0.18983063523769772
1101	94-95	0.23117324104457637
1101	96-97	0.21294652294703909
1101	98-99	0.23313529965315283
1101	100	0.21193897666184114
1102	1	0.17286241973770444
1102	2	0.1717065959763957
1102	3	0.16189550245682227
1102	4	0.0866738156028859
1102	5	0.025116836459481817
1102	6	0.08846665412822574
1102	7	0.08488686031725479
1102	8	0.12280401224469273
1102	9	0.13271258720051549
1102	10-11	0.11934447836917883
1102	12-13	0.13152260360807588
1102	14-15	0.285785117550553
1102	16-17	0.16084281454207883
1102	18-19	0.16456327126518744
1102	20-21	0.15183448664901533
1102	22-23	0.11322958459364685
1102	24-25	0.15079915499392627
1102	26-27	0.2135738755763299
1102	28-29	0.15731096346566176
1102	30-31	0.15503654483168816
1102	32-33	0.16568216133737934
1102	34-35	0.18713154745005767
1102	36-37	0.1870316007716042
1102	38-39	0.15743842804931063
1102	40-41	0.13821104661589345
1102	42-43	0.18850673208486057
1102	44-45	0.24832074471402166
1102	46-47	0.13366284332502687
1102	48-49	0.12127341507215306
1102	50-51	0.07118535869413734
1102	52-53	0.07860285075853568
1102	54-55	0.10029107360307421
1102	56-57	0.10612638706171396
1102	58-59	0.11783258970826438
1102	60-61	0.12111583235204648
1102	62-63	0.060187590107197764
1102	64-65	0.13268005720722442
1102	66-67	0.12095950088510676
1102	68-69	0.12678200594724842
1102	70-71	0.1278722094045861
1102	72-73	0.1035871384101199
1102	74-75	0.08018053314130213
1102	76-77	0.12218685680430497
1102	78-79	0.14045150436705
1102	80-81	0.1302740890233096
1102	82-83	0.08686416956513199
1102	84-85	0.09049264484791308
1102	86-87	0.07567450112978236
1102	88-89	0.09858849939712755
1102	90-91	0.07995952054856303
1102	92-93	0.08322118263770761
1102	94-95	0.11882360679434356
1102	96-97	0.12772930992531073
1102	98-99	0.10081875133950646
1102	100	0.05856275353666973
1103	1	0.13059875380538344
1103	2	0.14786608245729127
1103	3	0.0889668624296931
1103	4	0.08165867086106715
1103	5	0.06286781058636848
1103	6	0.06677211312474185
1103	7	0.08543770592124389
1103	8	0.05759454734739933
1103	9	0.06978505163431237
1103	10-11	0.02360901092615819
1103	12-13	0.07928468922505516
1103	14-15	0.1908770259666781
1103	16-17	0.10626322249662934
1103	18-19	0.14596572133710595
1103	20-21	0.14537645789094
1103	22-23	0.07425297265902486
1103	24-25	0.1658014041022824
1103	26-27	0.036462620457037076
1103	28-29	0.1058395871811939
1103	30-31	0.07104876924337589
1103	32-33	0.09125829508956684
1103	34-35	0.11631214053223005
1103	36-37	0.12566571225759304
1103	38-39	0.11522269524303397
1103	40-41	0.07995251095194789
1103	42-43	0.07305343172246381
1103	44-45	0.08944676977677801
1103	46-47	0.0457578991121963
1103	48-49	0.09171805185772541
1103	50-51	0.05106152012392329
1103	52-53	0.03787508467652145
1103	54-55	0.09350157626691669
1103	56-57	0.03537967335864067
1103	58-59	0.03039868637392118
1103	60-61	0.038600500306728236
1103	62-63	0.06119007224040729
1103	64-65	0.08156320111401527
1103	66-67	0.027178283956189375
1103	68-69	0.06417256543224426
1103	70-71	0.10127784264057027
1103	72-73	0.035401235801771236
1103	74-75	0.0704057126526152
1103	76-77	0.06959148873946219
1103	78-79	0.041728004495467985
1103	80-81	0.03444977814242378
1103	82-83	-0.03437315491449766
1103	84-85	0.020880605877550806
1103	86-87	0.011797446510570353
1103	88-89	-0.01146075186107609
1103	90-91	0.042131882461522
1103	92-93	0.06212412359244013
1103	94-95	-0.001784001616929487
1103	96-97	-0.00635830050574171
1103	98-99	0.022643943059261318
1103	100	0.009735665881496658
1104	1	0.10751631134497686
1104	2	0.1257704121392642
1104	3	0.0785112605662519
1104	4	0.07571187595854667
1104	5	-0.04053876576622173
1104	6	-0.006121156953454943
1104	7	0.04273615747140269
1104	8	0.009401976499724185
1104	9	0.03264474959284769
1104	10-11	-0.015612291348034546
1104	12-13	-0.018604530838679523
1104	14-15	0.1127065390236126
1104	16-17	0.03967888492923777
1104	18-19	-0.03742217454669117
1104	20-21	-0.00592108275873926
1104	22-23	-0.025491357420072802
1104	24-25	0.07595977071365922
1104	26-27	0.040918669100626914
1104	28-29	-0.07224090248627846
1104	30-31	-0.07270439797716222
1104	32-33	-0.04021870245953352
1104	34-35	-0.054733241328669635
1104	36-37	-0.051680574223006204
1104	38-39	-0.027418692304799208
1104	40-41	-0.007939782689454944
1104	42-43	-0.006172568092146946
1104	44-45	-0.027675149471733107
1104	46-47	0.014580319212122106
1104	48-49	0.048428277992059066
1104	50-51	-0.02876010731691281
1104	52-53	-0.05124242236571064
1104	54-55	0.02068315872638493
1104	56-57	0.0214663380799891
1104	58-59	0.01651568415453397
1104	60-61	-0.02578351811986579
1104	62-63	-0.09634666062471098
1104	64-65	-0.005672490652976592
1104	66-67	-0.036330895416256226
1104	68-69	-0.07072738856537342
1104	70-71	-0.13557881790072202
1104	72-73	-0.13132961385609576
1104	74-75	-0.14022674031021154
1104	76-77	-0.11837604527644885
1104	78-79	-0.05986038590762632
1104	80-81	-0.05720075129580238
1104	82-83	-0.04849734323081378
1104	84-85	0.005076206980952236
1104	86-87	-0.0694633195022547
1104	88-89	-0.019760014915863877
1104	90-91	-0.016201420894503826
1104	92-93	-0.04083548160042838
1104	94-95	-0.059742501202467935
1104	96-97	-0.05499094557086792
1104	98-99	-0.07455652446065741
1104	100	-0.1050344199295985
1105	1	0.08105348430893144
1105	2	0.07352227813590062
1105	3	0.03431575933351283
1105	4	0.035842180872876384
1105	5	0.03353811628435466
1105	6	-0.0029840656828241663
1105	7	-0.006570104331473203
1105	8	-0.026502950961614147
1105	9	0.009782955669706439
1105	10-11	-0.056207391728399614
1105	12-13	0.001665721472278392
1105	14-15	0.07177217012981885
1105	16-17	-0.015240492963663144
1105	18-19	-0.04502318474219891
1105	20-21	-0.08307427876375328
1105	22-23	-0.10693696311162881
1105	24-25	-0.012114080059355103
1105	26-27	-0.11339248231572441
1105	28-29	-0.08784047897924552
1105	30-31	-0.08601788288626011
1105	32-33	-0.11530058186824732
1105	34-35	-0.1671359170157558
1105	36-37	-0.05339162982797774
1105	38-39	-0.09621432330616386
1105	40-41	-0.05820418209144407
1105	42-43	-0.08521359763327041
1105	44-45	-0.04489009682691858
1105	46-47	-0.05154864650932467
1105	48-49	-0.06581724421785395
1105	50-51	-0.15179136440135466
1105	52-53	-0.05260965966578368
1105	54-55	-0.11903877267732099
1105	56-57	-0.15870346041290873
1105	58-59	-0.19463559778845507
1105	60-61	-0.15220716718837934
1105	62-63	-0.08121996960347388
1105	64-65	-0.018894882533757595
1105	66-67	-0.07509837110121254
1105	68-69	-0.1365318982462611
1105	70-71	-0.13155952619069922
1105	72-73	-0.12057340533046101
1105	74-75	-0.1910670053311989
1105	76-77	-0.16489604139860603
1105	78-79	-0.07199852992730627
1105	80-81	-0.12926600698807533
1105	82-83	-0.0865289850488935
1105	84-85	-0.05214178951459303
1105	86-87	-0.08696722382962463
1105	88-89	-0.07223195635083712
1105	90-91	-0.083530818869896
1105	92-93	-0.08242032310361225
1105	94-95	-0.1293004297759488
1105	96-97	-0.13628063084829023
1105	98-99	-0.1169252726852612
1105	100	-0.11211228310914478
1106	1	-0.02953933813728682
1106	2	-0.039097398695201235
1106	3	0.004786464333552232
1106	4	0.017818162185243125
1106	5	-0.044736841799249305
1106	6	-0.0633080621223101
1106	7	-0.07912017716665787
1106	8	-0.054726234332662216
1106	9	-0.028061725187988884
1106	10-11	-0.08762986913688309
1106	12-13	-0.06075977627862983
1106	14-15	0.04257829909056454
1106	16-17	-0.032020470617091235
1106	18-19	-0.035692753061773885
1106	20-21	-0.11200767595946104
1106	22-23	-0.10164583359682666
1106	24-25	-0.0366627078277304
1106	26-27	-0.11991118530554701
1106	28-29	-0.01910567345910863
1106	30-31	-0.005283718218194622
1106	32-33	-0.05254516049926394
1106	34-35	-0.03411788760430312
1106	36-37	-0.019289751996488746
1106	38-39	-0.02826927583326011
1106	40-41	-0.1476830616962559
1106	42-43	-0.057748479824041965
1106	44-45	-0.048479686336548866
1106	46-47	-0.03828228954466795
1106	48-49	-0.07835691066678407
1106	50-51	-0.07801500275153472
1106	52-53	-0.1068060337996215
1106	54-55	-0.07552098409244934
1106	56-57	-0.025594100463294467
1106	58-59	-0.0634654093560485
1106	60-61	-0.04280620621230469
1106	62-63	-0.12988487311994845
1106	64-65	-0.1715820967280024
1106	66-67	-0.07330382036911232
1106	68-69	-0.06228394054711828
1106	70-71	-0.03963339233347085
1106	72-73	-0.06688040811235396
1106	74-75	-0.06768652845153156
1106	76-77	-0.02786405328131991
1106	78-79	-0.04005870188079541
1106	80-81	-0.11000220221443158
1106	82-83	-0.045655442315371886
1106	84-85	6.552131130916905E-4
1106	86-87	-0.04713830883150649
1106	88-89	-0.057857538234607375
1106	90-91	-0.038017047553303485
1106	92-93	-0.027466487625368075
1106	94-95	-0.06819623783619022
1106	96-97	-0.08027940084851437
1106	98-99	-0.06858466825142884
1106	100	-0.08393908493816937
1107	1	-0.027909439020085358
1107	2	-0.08321985903728901
1107	3	-0.07234858067495509
1107	4	-0.07980870836789933
1107	5	-0.06207914892019062
1107	6	-0.13087890462315244
1107	7	-0.09950736559375883
1107	8	-0.15178762695842352
1107	9	-0.08911929101805072
1107	10-11	-0.11214497849405802
1107	12-13	-0.10692059808597776
1107	14-15	-0.0867250901307699
1107	16-17	-0.13141361786944827
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2208	14-15	0.7871174531158758
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2208	32-33	0.5994345541603607
2208	34-35	0.7197212181171366
2208	36-37	0.7296896929771961
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2208	40-41	0.7588466505853972
2208	42-43	0.7752746925282281
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2216	64-65	-0.5639933095277172
2216	66-67	-0.541357369752248
2216	68-69	-0.5312687404489935
2216	70-71	-0.5861571961022491
2216	72-73	-0.5038747305533917
2216	74-75	-0.478815984457853
2216	76-77	-0.5233698877932653
2216	78-79	-0.506404877599941
2216	80-81	-0.5752549696182285
2216	82-83	-0.5467972641189043
2216	84-85	-0.555264609458888
2216	86-87	-0.5953232733891944
2216	88-89	-0.624639588331501
2216	90-91	-0.6738254675202455
2216	92-93	-0.6471742613330491
2216	94-95	-0.6851711648557206
2216	96-97	-0.6410006106007664
2216	98-99	-0.6193299266409156
2216	100	-0.5779368027115517
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	704333.0
3	149681.0
4	88224.0
5	79722.0
6	83023.0
7	89532.0
8	94675.0
9	106551.0
10	119064.0
11	127521.0
12	133195.0
13	138112.0
14	140445.0
15	143347.0
16	145836.0
17	147758.0
18	150646.0
19	154521.0
20	160676.0
21	171259.0
22	191028.0
23	222076.0
24	272473.0
25	340558.0
26	425572.0
27	533330.0
28	662477.0
29	815044.0
30	1013249.0
31	1274864.0
32	1635605.0
33	2151563.0
34	2976465.0
35	4406403.0
36	7267164.0
37	1.2374544E7
38	1.698688E7
39	7327341.0
40	40833.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.29767749394517	28.819001785676452	26.229922719583637	21.65339800079474
2	23.461438612741382	28.259180825226693	27.664736874847375	20.614643687184547
3	23.962745388111692	26.56331685576746	27.064003855518205	22.409933900602645
4	24.105893972123006	26.27620131223961	26.544964169822066	23.072940545815317
5	24.05964404010373	26.562324075096917	26.427821552246893	22.95021033255246
6	23.999099354183436	26.373998660971537	25.791129470149166	23.83577251469586
7	23.77758893628891	26.434226264028215	26.519792643104545	23.26839215657833
8	23.20904266957381	26.63472363033268	26.69048933739736	23.465744362696146
9	23.1925245139813	26.31846339274061	26.819351376778883	23.66966071649921
10-11	23.20899415358444	26.622737912095385	26.962525350126022	23.20574258419415
12-13	23.159187431920543	26.494567727861813	27.0678483066272	23.27839653359044
14-15	23.179742762626706	26.406653128367687	27.127990938489866	23.28561317051574
16-17	23.259322324504875	26.351709483799564	27.003806664979855	23.385161526715706
18-19	23.29942519771908	26.196001469569218	26.931593475509715	23.572979857201986
20-21	23.3569721962789	26.147069079152335	26.863950224920348	23.632008499648414
22-23	23.454496149769096	26.158403086000337	26.785752643280762	23.601348120949805
24-25	23.512090383655472	26.091678277361847	26.66773280318641	23.728498535796273
26-27	23.559202909193054	26.085721481579377	26.634317477706553	23.720758131521013
28-29	23.58406654021376	26.136821956024363	26.597715696580632	23.681395807181246
30-31	23.67829480563426	26.0372003259702	26.5246792152136	23.759825653181945
32-33	23.702679388153005	26.05006594919465	26.46941794929527	23.777836713357075
34-35	23.736769572141544	26.074915735504746	26.466432071100225	23.721882621253485
36-37	23.752042656090598	25.97992330053096	26.421127871234606	23.846906172143832
38-39	23.773846467128138	25.95832112567894	26.427306081341612	23.84052632585131
40-41	23.811345975712623	26.006335644593104	26.370540162459655	23.811778217234618
42-43	23.865922511143317	25.915621696344637	26.313663000605906	23.90479279190614
44-45	23.865072495211276	25.90760802718058	26.313770535411273	23.91354894219687
46-47	23.90216303617	25.93917575812209	26.27142543425962	23.887235771448285
48-49	23.912888518725904	25.873357987939066	26.214491787165034	23.999261706169996
50-51	23.89286471669589	25.886071600045774	26.248541356939075	23.97252232631926
52-53	23.929509751538834	25.912502864687593	26.205406128663146	23.952581255110434
54-55	23.96229427043281	25.85714436152636	26.153387292393948	24.027174075646887
56-57	23.953362989538306	25.852352219316828	26.165936595342878	24.02834819580199
58-59	23.972300936334666	25.90067927675595	26.132915082904624	23.994104704004766
60-61	23.990872636596013	25.84519119168843	26.083550563376367	24.080385608339196
62-63	23.986409924063818	25.842026869668434	26.102106778663327	24.069456427604425
64-65	24.03220195092771	25.9024092783471	26.05969704847003	24.00569172225516
66-67	24.032722066127466	25.83211550341845	26.040070211918586	24.0950922185355
68-69	24.024391200079616	25.835978342006165	26.04154884678654	24.098081611127682
70-71	24.07302071827057	25.8786177421104	26.012573937759758	24.035787601859273
72-73	24.09332586696466	25.848924312721643	25.96485151267245	24.092898307641253
74-75	24.108115467398473	25.853312321369277	25.971482146918763	24.06709006431349
76-77	24.117499921039244	25.892987927629164	25.95746648810528	24.032045663226313
78-79	24.14383505963828	25.865057904869154	25.922123113084428	24.068983922408137
80-81	24.13179242914113	25.87531948807673	25.939147332759777	24.05374075002236
82-83	24.14874762801882	25.919064245393013	25.914938516953367	24.0172496096348
84-85	24.1750428134842	25.894709619588507	25.86469667966912	24.065550887258173
86-87	24.161689985262097	25.899095964458933	25.880723616157177	24.05849043412179
88-89	24.198560247278504	25.92135112488842	25.851304050245687	24.02878457758739
90-91	24.219162991539346	25.894790888295976	25.81562355511291	24.07042256505177
92-93	24.206891492789282	25.903407318708222	25.828522035171364	24.061179153331135
94-95	24.22998983683592	25.947711883720654	25.795508387233767	24.026789892209656
96-97	24.242481432980043	25.938947254145177	25.757654992792773	24.060916320082008
98-99	24.260876068819904	25.941717080652637	25.75226422314748	24.045142627379978
100	24.28126130689319	25.980043569495514	25.719985514669414	24.018709608941883
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13459.0
1	12796.5
2	13274.0
3	13803.5
4	10198.5
5	5117.5
6	2499.0
7	1920.0
8	1874.5
9	1925.0
10	1993.5
11	2184.0
12	2567.5
13	3098.0
14	4010.5
15	5496.5
16	8065.0
17	12467.0
18	19792.0
19	31107.0
20	47716.0
21	71064.0
22	102479.0
23	143706.5
24	195014.0
25	256996.0
26	330165.0
27	413175.5
28	504849.0
29	604488.5
30	708790.0
31	819475.0
32	937715.5
33	1063729.5
34	1199026.5
35	1341650.0
36	1491442.0
37	1656122.5
38	1832433.5
39	1998094.0
40	2135091.0
41	2228701.0
42	2268640.5
43	2260441.0
44	2215265.0
45	2136601.0
46	2029407.0
47	1916197.0
48	1809430.0
49	1713843.0
50	1636163.5
51	1573180.0
52	1522586.5
53	1493807.0
54	1491249.5
55	1505255.0
56	1527842.5
57	1538219.0
58	1522739.5
59	1486554.0
60	1435080.0
61	1369230.0
62	1289213.0
63	1195213.0
64	1088579.0
65	969544.0
66	845144.0
67	727423.5
68	617908.5
69	516077.0
70	425509.5
71	346660.0
72	279191.0
73	223044.5
74	176044.0
75	139067.0
76	110464.5
77	88181.0
78	71052.0
79	56168.0
80	44086.5
81	35074.5
82	27713.5
83	21470.5
84	16313.5
85	12106.0
86	8755.0
87	6202.0
88	4369.5
89	3008.5
90	2095.5
91	1456.0
92	978.0
93	693.5
94	506.5
95	378.5
96	276.0
97	207.5
98	166.5
99	150.5
100	299.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.21921259527783254
2	0.08224922278021016
3	0.08137484563730304
4	0.07015471322849864
5	0.06957543837132268
6	0.09429689069926595
7	0.10673490555711954
8	0.10772950955717639
9	0.09840021771990858
10-11	0.1194149355170278
12-13	0.1161422667821469
14-15	0.12436687678261688
16-17	0.11037918457773596
18-19	0.11626483572092941
20-21	0.11701664392505401
22-23	0.12462372506834585
24-25	0.12401009968055568
26-27	0.1222543191498431
28-29	0.12369157657849666
30-31	0.11377988710854253
32-33	0.12974429621149558
34-35	0.10945093955727475
36-37	0.11751706870059282
38-39	0.11358939780240919
40-41	0.11979591412929445
42-43	0.11528818768005727
44-45	0.1273116540889076
46-47	0.1281844698440595
48-49	0.11518357470045947
50-51	0.12875281498694913
52-53	0.13386714057907811
54-55	0.13995264935493607
56-57	0.12122458392529448
58-59	0.1349983660077142
60-61	0.13537778323222566
62-63	0.12620072670108903
64-65	0.12446446351731634
66-67	0.1335127055586497
68-69	0.1312807017626038
70-71	0.1369992844159918
72-73	0.12156730853755894
74-75	0.12164225514980813
76-77	0.1154466685372092
78-79	0.12380868066013602
80-81	0.13434726731379945
82-83	0.1266722658031568
84-85	0.12727340008890542
86-87	0.12899873355839175
88-89	0.14837087143704977
90-91	0.14138288053869127
92-93	0.13911730690590876
94-95	0.1591382013968487
96-97	0.14270849874284866
98-99	0.13697508290578633
100	0.12951555290536007
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6.404559E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.74161653992354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.97001850042031	60.60286877909924
2	15.181576940434102	23.301175120682792
3	4.028456776892885	9.274508556599102
4	1.1900981443370402	3.653202217503509
5	0.37621728429768353	1.4435761283632116
6	0.133129830205946	0.6129959027813903
7	0.04523158614570917	0.24298015276405718
8	0.02307770417419471	0.14168162594862746
9	0.011211160970945922	0.077432635457972
>10	0.03945760173378617	0.5692359750930674
>50	0.0014570689210612947	0.07368740589656182
>100	6.740146637851312E-5	0.006655499810516164
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.245551020765052E-6	1.561387755191263E-6	0.0	1.092971428633884E-5	0.0
2	9.368326531147577E-6	4.684163265573789E-6	0.0	1.7175265307103893E-5	0.0
3	1.4052489796721367E-5	1.092971428633884E-5	0.0	2.4982204083060207E-5	1.561387755191263E-6
4	1.7175265307103893E-5	1.2491102041530104E-5	3.122775510382526E-6	3.278914285901652E-5	6.245551020765052E-6
5	2.6543591838251472E-5	1.5613877551912628E-5	4.684163265573789E-6	3.9034693879781574E-5	6.245551020765052E-6
6	3.591191836939905E-5	1.8736653062295154E-5	4.684163265573789E-6	4.5280244900546626E-5	6.245551020765052E-6
7	4.371885714535536E-5	2.3420816327868946E-5	4.684163265573789E-6	5.3087183676502944E-5	9.368326531147577E-6
8	5.620995918688547E-5	2.6543591838251472E-5	6.245551020765052E-6	5.933273469726799E-5	9.368326531147577E-6
9	6.713967347322431E-5	4.059608163497284E-5	7.806938775956314E-6	6.401689796284178E-5	1.4052489796721367E-5
10-11	8.743771429071072E-5	5.152579592131168E-5	8.587632653551946E-6	7.572730612677626E-5	1.4052489796721367E-5
12-13	9.992881633224083E-5	6.323620408524614E-5	1.2491102041530104E-5	9.758673469945394E-5	1.4052489796721367E-5
14-15	1.1554269388415345E-4	7.338522449398936E-5	1.4052489796721367E-5	1.1632338776174908E-4	1.4833183674316997E-5
16-17	1.3662142857923551E-4	9.13411836786889E-5	1.7175265307103893E-5	1.3271795919125734E-4	2.029804081748642E-5
18-19	1.592615510295088E-4	9.992881633224084E-5	2.4201510205464575E-5	1.5535808164153067E-4	2.185942857267768E-5
20-21	1.7955959184699522E-4	1.0461297959781462E-4	3.2008448981420885E-5	1.756561224590171E-4	2.3420816327868946E-5
22-23	2.107873469508205E-4	1.1710408163934472E-4	3.4350530614207786E-5	1.92831387766121E-4	2.3420816327868946E-5
24-25	2.568482857289628E-4	1.2959518368087483E-4	3.5131224491803415E-5	2.0688387756284234E-4	2.3420816327868946E-5
26-27	3.1071616328306133E-4	1.4052489796721366E-4	4.293816326775973E-5	2.256205306251375E-4	2.654359183825147E-5
28-29	3.825400000218594E-4	1.491125306207656E-4	4.9183714288524785E-5	2.357695510338807E-4	3.1227755103825256E-5
30-31	4.7700395921093083E-4	1.655071020502739E-4	5.855204081967236E-5	2.4826065307541085E-4	3.591191836939905E-5
32-33	5.816169388087454E-4	1.8034028572459088E-4	6.479759184043741E-5	2.6543591838251474E-4	3.669261224699468E-5
34-35	6.596863265683087E-4	1.967348571540991E-4	6.792036735081994E-5	2.779270204240448E-4	4.059608163497284E-5
36-37	7.736676326972708E-4	2.084452653180336E-4	7.650800000437189E-5	2.919795102207662E-4	4.371885714535536E-5
38-39	9.204380816852495E-4	2.154715102163943E-4	7.96307755147544E-5	3.0368991838470064E-4	4.449955102295099E-5
40-41	0.0010570595102644851	2.2718191838032878E-4	8.275355102513694E-5	3.2086518369180454E-4	5.152579592131168E-5
42-43	0.0012514522857857973	2.4982204083060205E-4	8.353424490273258E-5	3.4038253063169534E-4	5.386787755409857E-5
44-45	0.0014825376735541043	2.7012008164808846E-4	8.743771429071072E-5	3.5989987757158615E-4	5.6990653064481096E-5
46-47	0.0017557805307125752	2.810497959344273E-4	9.446395918907141E-5	3.794172245114769E-4	5.9332734697267996E-5
48-49	0.0020352689388918113	2.9354089797595744E-4	9.524465306666704E-5	3.895662449202201E-4	6.401689796284178E-5
50-51	0.002374870775645911	3.0837408165027447E-4	9.836742857704957E-5	4.036187347169415E-4	6.792036735081994E-5
52-53	0.0027948840817923607	3.2320726532459145E-4	1.0070951020983647E-4	4.2235538777923664E-4	7.338522449398936E-5
54-55	0.003317948979781434	3.333562857333346E-4	1.0305159184262336E-4	4.3328510206557544E-4	8.19728571475413E-5
56-57	0.003915960490019688	3.450666938972691E-4	1.0773575510819715E-4	4.465568979847012E-4	8.66570204131151E-5
58-59	0.0045491032247497445	3.5755779593879924E-4	1.1085853061857968E-4	4.66074244924592E-4	9.524465306666704E-5
60-61	0.005220499959481988	3.731716734907118E-4	1.1554269388415345E-4	4.848108979868871E-4	9.992881633224084E-5
62-63	0.005984018571770515	3.864434694098376E-4	1.2100755102732288E-4	4.95740612273226E-4	1.0227089796502773E-4
64-65	0.0068576150208000276	3.91908326553007E-4	1.2334963266010977E-4	5.207228163562861E-4	1.0539367347541025E-4
66-67	0.007849096245346479	4.06741510227324E-4	1.288144898032792E-4	5.472664081945377E-4	1.1085853061857968E-4
68-69	0.009045899959700582	4.1532914288087595E-4	1.3037587755847046E-4	5.620995918688546E-4	1.1476200000655782E-4
70-71	0.010437096449575998	4.3484648982076676E-4	1.3115657143606608E-4	5.855204081967236E-4	1.1710408163934472E-4
72-73	0.012003168368032835	4.5202175512787065E-4	1.3271795919125737E-4	6.003535918710407E-4	1.1944616327213162E-4
74-75	0.01385887771507765	4.6919702043497454E-4	1.389635102120224E-4	6.222130204437183E-4	1.2178824490491851E-4
76-77	0.0161018112254099	4.887143673748652E-4	1.4208628572240492E-4	6.479759184043741E-4	1.2491102041530103E-4
78-79	0.01878427538882849	5.152579592131168E-4	1.4442836735519183E-4	6.737388163650299E-4	1.3037587755847046E-4
80-81	0.022211521511473313	5.300911428874338E-4	1.4989322449836124E-4	6.909140816721338E-4	1.3584073470163989E-4
82-83	0.02655452155253781	5.433629388065595E-4	1.5535808164153067E-4	7.197997551431723E-4	1.3740212245683114E-4
84-85	0.032084176287547665	5.542926530928984E-4	1.5848085715191318E-4	7.400977959606586E-4	1.4052489796721366E-4
86-87	0.03936024322673895	5.714679184000023E-4	1.6004224490710447E-4	7.71325551064484E-4	1.4286697960000057E-4
88	0.046229568655702914	5.792748571759585E-4	1.6238432653989135E-4	7.885008163715878E-4	1.436476734775962E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	12005	0.0	5.5216503	94
AGATCGG	14895	0.0	5.2078133	94
>>END_MODULE