##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049541_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	64045590
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50014155541388	34.0	31.0	34.0	31.0	34.0
2	32.70275736393403	34.0	31.0	34.0	31.0	34.0
3	32.75674724832732	34.0	31.0	34.0	31.0	34.0
4	36.16883285796883	37.0	35.0	37.0	35.0	37.0
5	36.07147750532082	37.0	35.0	37.0	35.0	37.0
6	36.0686137796529	37.0	35.0	37.0	35.0	37.0
7	36.073991480131575	37.0	35.0	37.0	35.0	37.0
8	36.06760654402591	37.0	35.0	37.0	35.0	37.0
9	37.81495709540657	39.0	38.0	39.0	35.0	39.0
10-11	37.80730977730083	39.0	38.0	39.0	35.0	39.0
12-13	37.80324341613529	39.0	38.0	39.0	35.0	39.0
14-15	39.26230445374928	41.0	39.0	41.0	36.0	41.0
16-17	39.24249581430978	41.0	39.0	41.0	36.0	41.0
18-19	39.24083841682152	41.0	39.0	41.0	36.0	41.0
20-21	39.2429838338596	41.0	39.0	41.0	36.0	41.0
22-23	39.19325542164574	41.0	39.0	41.0	36.0	41.0
24-25	39.150512619213906	41.0	39.0	41.0	35.5	41.0
26-27	39.07554302333697	41.0	39.0	41.0	35.0	41.0
28-29	39.003777051628376	41.0	39.0	41.0	35.0	41.0
30-31	38.9350451139571	41.0	38.5	41.0	35.0	41.0
32-33	38.87047162029423	41.0	38.0	41.0	35.0	41.0
34-35	38.79785053116069	40.0	38.0	41.0	35.0	41.0
36-37	38.72457152787569	40.0	38.0	41.0	35.0	41.0
38-39	38.657919382115146	40.0	38.0	41.0	34.0	41.0
40-41	38.575063123003474	40.0	38.0	41.0	34.0	41.0
42-43	38.47744270917014	40.0	38.0	41.0	34.0	41.0
44-45	38.37598584851822	40.0	38.0	41.0	34.0	41.0
46-47	38.25300707667772	40.0	38.0	41.0	33.0	41.0
48-49	38.13333909485415	40.0	37.0	41.0	33.0	41.0
50-51	37.99328369837798	40.0	37.0	41.0	33.0	41.0
52-53	37.822859981460084	40.0	36.5	41.0	33.0	41.0
54-55	37.64922206197179	40.0	36.0	41.0	33.0	41.0
56-57	37.4398859000284	39.5	35.5	41.0	32.5	41.0
58-59	37.238603875770366	39.0	35.0	41.0	32.0	41.0
60-61	37.008920028685814	39.0	35.0	41.0	32.0	41.0
62-63	36.75360842175082	38.5	35.0	41.0	31.5	41.0
64-65	36.585965450548585	38.0	35.0	41.0	31.0	41.0
66-67	36.31126495985126	37.0	35.0	40.0	31.0	41.0
68-69	36.026316769039056	37.0	35.0	40.0	31.0	41.0
70-71	35.729081159217984	36.0	35.0	39.0	31.0	41.0
72-73	35.48378992214764	36.0	35.0	39.0	31.0	41.0
74-75	35.177003201937865	35.5	35.0	39.0	31.0	40.5
76-77	34.84755027629537	35.0	34.0	37.5	31.0	39.5
78-79	34.55670792477671	35.0	34.0	37.0	30.5	39.0
80-81	34.260754846664696	35.0	34.0	36.5	30.0	39.0
82-83	33.929551238422505	35.0	34.0	36.0	30.0	38.0
84-85	33.716729106562994	35.0	34.0	36.0	30.0	37.0
86-87	33.523562566915224	35.0	34.0	35.5	30.0	37.0
88-89	33.34345917494085	35.0	34.0	35.0	30.0	36.0
90-91	33.1870607328311	35.0	34.0	35.0	30.0	36.0
92-93	33.043698847961274	35.0	34.0	35.0	29.5	36.0
94-95	32.90147329425804	35.0	34.0	35.0	29.0	36.0
96-97	32.75126612933069	35.0	34.0	35.0	29.0	35.5
98-99	32.631929536444275	35.0	34.0	35.0	29.0	35.0
100	32.54447021254703	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17398982464004575
1101	2	0.15339936646604713
1101	3	0.13865911978628986
1101	4	0.14730807676751567
1101	5	0.1320980187250882
1101	6	0.13699627925280566
1101	7	0.16513541893860406
1101	8	0.16798700256465793
1101	9	0.2342205963305375
1101	10-11	0.21011211570056076
1101	12-13	0.18430451603458664
1101	14-15	0.24114097035054272
1101	16-17	0.2818987897100129
1101	18-19	0.25027418071694996
1101	20-21	0.2029375472766617
1101	22-23	0.2899290613741954
1101	24-25	0.24647313434650897
1101	26-27	0.2890101582482032
1101	28-29	0.29109366115991264
1101	30-31	0.285882837991835
1101	32-33	0.27370918494899854
1101	34-35	0.31355660436462784
1101	36-37	0.330815752621902
1101	38-39	0.2988261600912807
1101	40-41	0.2639049251070773
1101	42-43	0.3050122659329304
1101	44-45	0.29503522612119326
1101	46-47	0.21413659103557592
1101	48-49	0.26533671382263435
1101	50-51	0.29450248943910395
1101	52-53	0.24115322588744448
1101	54-55	0.23192436638268532
1101	56-57	0.21209901302681544
1101	58-59	0.25511518281278
1101	60-61	0.24902041350347304
1101	62-63	0.2688460786607294
1101	64-65	0.21930875841591302
1101	66-67	0.2764521613326707
1101	68-69	0.2547862735373201
1101	70-71	0.23170370564816523
1101	72-73	0.25373317892280056
1101	74-75	0.23801630684534558
1101	76-77	0.20948775532141184
1101	78-79	0.23790209818097452
1101	80-81	0.224099643035224
1101	82-83	0.26992972124006087
1101	84-85	0.2623264829270653
1101	86-87	0.2048528772498912
1101	88-89	0.2164101500798239
1101	90-91	0.25107248855717046
1101	92-93	0.23575643478302055
1101	94-95	0.2124218107541438
1101	96-97	0.239454083487594
1101	98-99	0.2100332997137926
1101	100	0.25926675611319894
1102	1	0.11210292870194394
1102	2	0.12399327519131731
1102	3	0.09315293627442145
1102	4	0.10566222531690528
1102	5	0.11726930878933928
1102	6	0.1682626417367601
1102	7	0.13630727541281118
1102	8	0.11849971601471765
1102	9	0.1814587103848737
1102	10-11	0.16629630791793204
1102	12-13	0.15791146981769089
1102	14-15	0.15266288853500498
1102	16-17	0.1957314949849902
1102	18-19	0.2358573062114644
1102	20-21	0.2045636485255713
1102	22-23	0.2532510597361437
1102	24-25	0.2649082597073047
1102	26-27	0.20958371390799613
1102	28-29	0.24107755419392163
1102	30-31	0.24151786888121052
1102	32-33	0.25290423302465115
1102	34-35	0.31053389497723316
1102	36-37	0.24353054051965017
1102	38-39	0.2104150501632489
1102	40-41	0.2429594870103955
1102	42-43	0.2306551494478697
1102	44-45	0.172985978051436
1102	46-47	0.22187611628080361
1102	48-49	0.24357452615898723
1102	50-51	0.23310674013017518
1102	52-53	0.23982698569545136
1102	54-55	0.2623450976594839
1102	56-57	0.2871318027735157
1102	58-59	0.27582479479915634
1102	60-61	0.2706169308468702
1102	62-63	0.24221868088781662
1102	64-65	0.21914805081552657
1102	66-67	0.21764576112435918
1102	68-69	0.2120804913943104
1102	70-71	0.1804247271849917
1102	72-73	0.11943793034981098
1102	74-75	0.1773021215165258
1102	76-77	0.18497524185271885
1102	78-79	0.1367080990937879
1102	80-81	0.15226313350581222
1102	82-83	0.2278877236762753
1102	84-85	0.24037350552455194
1102	86-87	0.13258223322904428
1102	88-89	0.08486305983592501
1102	90-91	0.13680157344923316
1102	92-93	0.13588625359779627
1102	94-95	0.12676189166904095
1102	96-97	0.20753911781117296
1102	98-99	0.20219807296624737
1102	100	0.18357766909064566
1103	1	0.07213022424043913
1103	2	0.06319142672113287
1103	3	0.07086590593166875
1103	4	0.07942725522415373
1103	5	0.09764694302151611
1103	6	0.13397027229563463
1103	7	0.10936037799740461
1103	8	0.0874599683590489
1103	9	0.10334819089906233
1103	10-11	0.13583866160414004
1103	12-13	0.10823433197231225
1103	14-15	0.1285144442896211
1103	16-17	0.13367775175419183
1103	18-19	0.1455609663702404
1103	20-21	0.15322936002210952
1103	22-23	0.16914993593718464
1103	24-25	0.1736534049244156
1103	26-27	0.16426141534024907
1103	28-29	0.20660583669732802
1103	30-31	0.14870802280981366
1103	32-33	0.15027550054816174
1103	34-35	0.17095932342407139
1103	36-37	0.14476406504181227
1103	38-39	0.1312929062283743
1103	40-41	0.1704812874982906
1103	42-43	0.11295973501293588
1103	44-45	0.12065576770603315
1103	46-47	0.15296812270247528
1103	48-49	0.19702932060688028
1103	50-51	0.158316614511385
1103	52-53	0.18429000615040536
1103	54-55	0.18953669185179223
1103	56-57	0.20686250953776408
1103	58-59	0.16859632906233202
1103	60-61	0.14995581132058078
1103	62-63	0.2042834554606543
1103	64-65	0.15870513723899649
1103	66-67	0.1266124632422958
1103	68-69	0.14521653517656574
1103	70-71	0.1470902255192854
1103	72-73	0.15874611812502337
1103	74-75	0.16457866266787136
1103	76-77	0.12954178383651538
1103	78-79	0.11742161680970753
1103	80-81	0.08957531879339342
1103	82-83	0.12402949892111081
1103	84-85	0.08985032215451838
1103	86-87	0.08616136416199538
1103	88-89	0.11372774014048304
1103	90-91	0.07341156533389892
1103	92-93	0.09496386210870611
1103	94-95	0.11265096218543391
1103	96-97	0.17319067745538064
1103	98-99	0.15880521149756532
1103	100	0.1402085425169446
1104	1	0.0551290576354333
1104	2	0.030316842431574287
1104	3	0.021149669025284368
1104	4	0.0571724193787162
1104	5	0.0840257724259601
1104	6	0.07815776991260748
1104	7	0.10219352471959553
1104	8	0.0934549610661648
1104	9	0.07550592078509766
1104	10-11	0.07961198570631467
1104	12-13	0.0980868022201804
1104	14-15	0.10307885630790281
1104	16-17	0.11073227115836914
1104	18-19	0.02795485841472356
1104	20-21	-0.004523436401818515
1104	22-23	-0.011742654816465858
1104	24-25	0.05269816742182343
1104	26-27	0.06951080179118208
1104	28-29	0.044655455315655956
1104	30-31	0.029264277501376057
1104	32-33	0.08373281088422857
1104	34-35	0.06998137803848437
1104	36-37	0.07534858197409733
1104	38-39	0.12002464123242618
1104	40-41	0.10117906341208993
1104	42-43	0.13655865320116334
1104	44-45	0.09179470029068426
1104	46-47	0.10889960145662769
1104	48-49	0.10502064582389892
1104	50-51	0.1338026248261599
1104	52-53	0.13225006588390187
1104	54-55	0.1481165000939555
1104	56-57	0.09240910772353317
1104	58-59	0.09613836154743893
1104	60-61	0.07515551276470234
1104	62-63	0.13878286015341246
1104	64-65	0.09474131947263942
1104	66-67	0.11302343112002688
1104	68-69	0.12693840254992494
1104	70-71	0.11560465817095888
1104	72-73	0.08348709476275218
1104	74-75	0.08070281890798725
1104	76-77	0.14358493159699748
1104	78-79	0.1020057182572387
1104	80-81	0.07491903034861025
1104	82-83	0.1633190823434063
1104	84-85	0.15484429536593325
1104	86-87	0.10291698697442087
1104	88-89	0.09236518645698766
1104	90-91	0.07059298816864157
1104	92-93	0.05589575216338716
1104	94-95	0.07085548745899928
1104	96-97	0.06700535797356366
1104	98-99	0.03143844933887152
1104	100	0.028711969811794802
1105	1	-0.036077329772325584
1105	2	-0.020625280194757067
1105	3	-0.03132307348004559
1105	4	0.038707022558647
1105	5	-0.01096914066795307
1105	6	0.008440274992814523
1105	7	-0.016627309540254487
1105	8	-0.009460628300303142
1105	9	-0.03885706921956711
1105	10-11	-0.028151427014620367
1105	12-13	0.0024798780798391817
1105	14-15	0.007632398336113511
1105	16-17	0.017407217734970004
1105	18-19	0.012456186540319436
1105	20-21	0.03441229560939263
1105	22-23	0.010894666186700874
1105	24-25	0.007153984058504648
1105	26-27	0.036622736417200485
1105	28-29	0.06103537085408561
1105	30-31	0.028615034943030082
1105	32-33	0.04667182405727033
1105	34-35	0.07193473140367956
1105	36-37	0.08822631044498763
1105	38-39	0.0873190504072241
1105	40-41	0.09372977350789569
1105	42-43	0.09150556863605175
1105	44-45	0.09703474279750424
1105	46-47	0.03903486399156719
1105	48-49	0.04028667854612422
1105	50-51	-0.01129203398630807
1105	52-53	0.027206981027035226
1105	54-55	0.039784940114749645
1105	56-57	0.03757261216725283
1105	58-59	0.06684054967914932
1105	60-61	0.06378138987920323
1105	62-63	0.04949960188397995
1105	64-65	0.07427361282078238
1105	66-67	0.08092297591174713
1105	68-69	0.04773642848360993
1105	70-71	0.044523582379540017
1105	72-73	0.09766779243139467
1105	74-75	0.08702190834306123
1105	76-77	0.10656364085582481
1105	78-79	0.09907563026257549
1105	80-81	0.08470784108067164
1105	82-83	0.1297808131018101
1105	84-85	0.11301874768485476
1105	86-87	0.0688389336941384
1105	88-89	0.051736504471826095
1105	90-91	0.08157247918816779
1105	92-93	0.06238774336266317
1105	94-95	0.08526666973967423
1105	96-97	0.09711332723770738
1105	98-99	0.07220311963195059
1105	100	0.040501658783774985
1106	1	-0.07580247708214927
1106	2	-0.05459412303106603
1106	3	-0.0489563714513892
1106	4	-0.004311046582550659
1106	5	0.016181395756944994
1106	6	0.0065790183821334836
1106	7	-0.0010400098140763703
1106	8	-0.05586135257685498
1106	9	-0.03403710197577681
1106	10-11	-0.0384261661980716
1106	12-13	-6.718452610527947E-4
1106	14-15	-0.06596328209057845
1106	16-17	-0.06375160192813922
1106	18-19	0.003020478749000688
1106	20-21	-6.216560169036711E-4
1106	22-23	-0.02251543580180737
1106	24-25	-0.028982237975952785
1106	26-27	0.023903457950730456
1106	28-29	0.04314383958970325
1106	30-31	0.01971142912154278
1106	32-33	0.023589036225125426
1106	34-35	0.032328623791386235
1106	36-37	0.021155159072968388
1106	38-39	0.03881312162656059
1106	40-41	0.017557914507783323
1106	42-43	0.012337142251595878
1106	44-45	0.0379005541047448
1106	46-47	0.036884267077972765
1106	48-49	0.03379226971242133
1106	50-51	0.04602320146330641
1106	52-53	0.06435138773986182
1106	54-55	0.00432299534866587
1106	56-57	0.03071131783376302
1106	58-59	0.05622708173942925
1106	60-61	0.03801743620962128
1106	62-63	0.029392135561899124
1106	64-65	0.043826448383541106
1106	66-67	0.04946385331916048
1106	68-69	0.013101447609422223
1106	70-71	0.0024266213705033124
1106	72-73	0.036974016285839184
1106	74-75	0.03509265755966595
1106	76-77	0.03306255536136149
1106	78-79	0.02136261526465688
1106	80-81	0.027281064178566794
1106	82-83	0.1003553150752623
1106	84-85	0.06655864944723788
1106	86-87	0.0445185966808026
1106	88-89	0.03061387691978723
1106	90-91	0.017672661845992366
1106	92-93	0.04370701273936817
1106	94-95	-0.0016317534533030198
1106	96-97	0.038180532175083215
1106	98-99	0.026991150690641064
1106	100	0.022432168796129304
1107	1	-0.07600864094566617
1107	2	-0.05637527081443494
1107	3	-0.07273160470448659
1107	4	-0.013005338246180997
1107	5	-0.025450209352612774
1107	6	-0.06360647936119079
1107	7	-0.030850098163490713
1107	8	-0.03156061752394379
1107	9	-0.01691061520098458
1107	10-11	-0.02864232004070999
1107	12-13	-0.02093643787235777
1107	14-15	-0.043480337244830025
1107	16-17	-0.027528074732515506
1107	18-19	-0.030086151893122803
1107	20-21	-0.06924080258314547
1107	22-23	-0.04337643441674999
1107	24-25	-0.0779749325742145
1107	26-27	-0.02868983048382745
1107	28-29	-0.01157104026401612
1107	30-31	0.00955333894496846
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2216	70-71	-0.6488652514973907
2216	72-73	-0.5195756428672809
2216	74-75	-0.47083820871718984
2216	76-77	-0.4708519479680078
2216	78-79	-0.5123197748254995
2216	80-81	-0.5806029947698335
2216	82-83	-0.5525185193339581
2216	84-85	-0.5884939970289693
2216	86-87	-0.6072827821535043
2216	88-89	-0.6234716593783602
2216	90-91	-0.6549681629244759
2216	92-93	-0.7937768647740384
2216	94-95	-0.7454803094737557
2216	96-97	-0.7399196271444808
2216	98-99	-0.79364783302319
2216	100	-0.8183194372114322
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	116.0
3	181.0
4	407.0
5	958.0
6	2331.0
7	5850.0
8	13115.0
9	21491.0
10	29561.0
11	37048.0
12	42059.0
13	47760.0
14	52361.0
15	57137.0
16	61654.0
17	66015.0
18	71060.0
19	78667.0
20	87815.0
21	99848.0
22	118078.0
23	143912.0
24	181884.0
25	237627.0
26	314053.0
27	416519.0
28	548349.0
29	709466.0
30	908089.0
31	1157557.0
32	1487258.0
33	1948542.0
34	2699220.0
35	4083225.0
36	7110927.0
37	1.3188293E7
38	1.9157405E7
39	8796452.0
40	63300.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.241752430027674	27.40199894870492	25.70354732294815	23.652701298319258
2	22.45004712244677	27.41183035357021	27.238178027830934	22.899944496152088
3	22.69278497891274	26.58218323628077	27.306397633921005	23.418634150885488
4	23.43162735409442	26.06048478956119	26.737194708237013	23.770693148107387
5	23.93594845431911	26.06462615753059	26.377168160407642	23.62225722774266
6	23.790304491867563	25.894583663207033	25.910146534733116	24.404965310192296
7	23.526174089425986	26.168705761005555	26.414771102897173	23.890349046671286
8	23.145963679934873	26.279064647542477	26.507882900290248	24.067088772232406
9	23.11792271723939	26.05675738173386	26.68109545091239	24.14422445011436
10-11	23.070737579277512	26.38094754065034	26.81906357643048	23.72925130364167
12-13	22.966705435924627	26.256268386316684	26.961575340316173	23.815450837442516
14-15	23.030413179111942	26.224862633008765	26.94473108921317	23.79999309866612
16-17	23.11733641613732	26.175865504556988	26.890045825169228	23.816752254136468
18-19	23.140138756803896	26.029540238873516	26.857817248306308	23.972503756016277
20-21	23.19496479218339	26.00847983589937	26.81510303779126	23.981452334125983
22-23	23.28855538578393	26.054786600666517	26.72161238334351	23.935045630206044
24-25	23.350925666342597	25.992059951023457	26.643728868843592	24.013285513790354
26-27	23.430055300273033	25.984683600397005	26.607569639670203	23.977691459659752
28-29	23.488846607753974	26.029704078194943	26.546463269097437	23.93498604495364
30-31	23.54325868808002	25.9512031685087	26.509885960061936	23.995652183349343
32-33	23.595446758142923	25.957205042687185	26.44962485757223	23.99772334159766
34-35	23.621063233611935	25.98726056669236	26.415805584275887	23.975870615419815
36-37	23.646875882086825	25.9290752462083	26.379215697773677	24.0448331739312
38-39	23.664582526291035	25.91271389646032	26.379872525180893	24.042831052067754
40-41	23.69523675600639	25.949768530451273	26.338249334168022	24.016745379374314
42-43	23.744986667676365	25.88925525183473	26.28369061202216	24.082067468466743
44-45	23.752774859977052	25.871973604296215	26.280328910784682	24.094922624942043
46-47	23.799237535294683	25.906303068888235	26.23220530219978	24.062254093617298
48-49	23.808230794381128	25.840536536635316	26.215168946865653	24.136063722117903
50-51	23.808312953319657	25.86353408564118	26.1989396928032	24.129213268235954
52-53	23.843856260911558	25.894452155605496	26.17904743709317	24.08264414638977
54-55	23.873186202090494	25.844753766481272	26.14553718034526	24.13652285108298
56-57	23.870655475559968	25.848989916946085	26.14739439432561	24.13296021316834
58-59	23.885184851105944	25.88015021740847	26.12988410611925	24.104780825366337
60-61	23.91531133345414	25.836617697119834	26.084035223648133	24.16403574577789
62-63	23.90460290655773	25.846601113375172	26.082822019195074	24.165973960872023
64-65	23.93437777432444	25.886343606728822	26.068079508861892	24.11119911008484
66-67	23.958721131081916	25.847653695943663	26.030655068028473	24.16297010494595
68-69	23.940130054055455	25.84854760261697	26.03400787280635	24.177314470521228
70-71	23.98531515395865	25.8764825623855	26.01582583939852	24.12237644425733
72-73	23.99179923953803	25.86512932725097	25.973910957997216	24.169160475213786
74-75	24.006208077683443	25.866327531917975	25.98824659794389	24.139217792454694
76-77	24.02505290444487	25.898433255849472	25.964368978072187	24.11214486163347
78-79	24.02680990267855	25.88083808840128	25.95037855896047	24.141973449959696
80-81	24.027274839667264	25.892925043914484	25.950155158370258	24.129644958047997
82-83	24.04282182837638	25.928239787986552	25.93490379399521	24.09403458964185
84-85	24.036009291804785	25.901992177604384	25.91478855502808	24.147209975562753
86-87	24.03100080425289	25.912849203113858	25.923026496625816	24.133123496007432
88-89	24.05136663753446	25.938612733890494	25.902259606268107	24.10776102230694
90-91	24.057187665149183	25.91616518644779	25.87816162570331	24.148485522699715
92-93	24.041061217365392	25.937298290207096	25.88583327309616	24.135807219331355
94-95	24.059689588979825	25.972732363926127	25.864114254559194	24.103463792534853
96-97	24.06394241887112	25.967325112050542	25.82903535062913	24.13969711844921
98-99	24.050526746864154	25.99106551704346	25.834302903476658	24.124104832615732
100	24.086347265858844	26.012111107537255	25.804445725481358	24.097095901122547
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	102.0
1	80.5
2	64.0
3	64.5
4	73.0
5	104.0
6	136.5
7	179.5
8	248.5
9	338.5
10	445.0
11	590.0
12	832.0
13	1277.5
14	1953.5
15	3079.0
16	5123.5
17	8679.5
18	14614.5
19	23825.0
20	37730.5
21	57867.5
22	85444.5
23	121791.0
24	168291.0
25	224288.5
26	291385.5
27	369769.5
28	457737.5
29	554285.5
30	657565.0
31	770095.5
32	891184.0
33	1022666.5
34	1165098.5
35	1316383.5
36	1479000.0
37	1656731.0
38	1844592.5
39	2022503.0
40	2171850.0
41	2273021.5
42	2315715.0
43	2309497.0
44	2263001.5
45	2181017.0
46	2071817.5
47	1957486.0
48	1849574.0
49	1752723.0
50	1675187.5
51	1611732.0
52	1561512.0
53	1532077.0
54	1527821.0
55	1542974.5
56	1563036.0
57	1569008.0
58	1551811.0
59	1512378.0
60	1456345.0
61	1384755.0
62	1298993.0
63	1201414.5
64	1088079.0
65	962689.0
66	835344.0
67	715296.0
68	604314.5
69	501510.0
70	410241.5
71	331913.0
72	265186.0
73	208411.5
74	162651.0
75	128097.5
76	100597.0
77	79236.0
78	62810.0
79	49301.5
80	38634.5
81	30339.0
82	23517.0
83	17693.0
84	12850.0
85	8985.5
86	6289.0
87	4231.5
88	2663.5
89	1605.5
90	953.5
91	558.5
92	291.5
93	158.5
94	90.0
95	59.5
96	35.0
97	20.5
98	13.0
99	5.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10814327731230207
2	0.007783517959628446
3	8.275355102513694E-5
4	0.1665563546217624
5	0.0036817523267409983
6	0.0033398084083541115
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.006767054530998934
20-21	1.7175265307103893E-5
22-23	1.1866546939453598E-4
24-25	0.0010070951020983644
26-27	0.0015137654286579295
28-29	7.471240408590193E-4
30-31	8.915524082142111E-4
32-33	0.001324056816402191
34-35	4.3172371431038424E-4
36-37	3.747330612459031E-5
38-39	0.0
40-41	5.316525306426251E-4
42-43	1.7955959184699522E-4
44-45	2.4982204083060207E-5
46-47	3.903469387978157E-6
48-49	1.5613877551912628E-5
50-51	0.0
52-53	3.989345714513677E-4
54-55	6.245551020765052E-6
56-57	0.0012600399184393492
58-59	2.6543591838251472E-5
60-61	3.247686530797827E-4
62-63	3.786365306338813E-4
64-65	0.002002479796032795
66-67	0.001116392244961753
68-69	1.0461297959781463E-4
70-71	8.111409388218611E-4
72-73	4.840302041092915E-4
74-75	0.0010055337143431735
76-77	0.001000068857200004
78-79	2.3498885715628507E-4
80-81	0.002358476204216403
82-83	4.6060938778142255E-5
84-85	1.881472245005472E-4
86-87	0.0016519482449923564
88-89	3.224265714469958E-4
90-91	4.30162326555193E-4
92-93	2.2405914286994624E-4
94-95	0.0015941768980502794
96-97	0.0011218571021049226
98-99	4.059608163497284E-5
100	3.903469387978157E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6.404559E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.90348445679037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.79705252717262	59.05067367280352
2	16.053164231148997	24.369822034026424
3	4.239004400181401	9.652656138043051
4	1.2546815311853188	3.8093880040218715
5	0.40492862986613476	1.5367746981576789
6	0.13790134121226275	0.6280315385565319
7	0.05026526646728658	0.2670716210411279
8	0.01886418964144662	0.11454861801915919
9	0.008096219570175756	0.05530781486732377
>10	0.03571260118394893	0.4915145675268943
>50	2.977542245517126E-4	0.014736006439941397
>100	2.9246129388645507E-5	0.003410359799895408
>500	0.0	0.0
>1k	2.0620164996442894E-6	0.00606492669655283
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.684163265573789E-6	1.561387755191263E-6	4.684163265573789E-6	1.7175265307103893E-5	1.561387755191263E-6
2	6.245551020765052E-6	6.245551020765052E-6	6.245551020765052E-6	2.4982204083060207E-5	3.122775510382526E-6
3	9.368326531147577E-6	1.2491102041530104E-5	7.806938775956314E-6	3.9034693879781574E-5	3.122775510382526E-6
4	1.2491102041530104E-5	1.8736653062295154E-5	9.368326531147577E-6	4.9964408166120414E-5	3.122775510382526E-6
5	2.4982204083060207E-5	2.6543591838251472E-5	1.4052489796721367E-5	7.806938775956315E-5	4.684163265573789E-6
6	2.6543591838251472E-5	2.8104979593442733E-5	1.5613877551912628E-5	9.056048980109325E-5	6.245551020765052E-6
7	2.8104979593442733E-5	3.1227755103825256E-5	2.185942857267768E-5	1.014902040874321E-4	6.245551020765052E-6
8	3.1227755103825256E-5	3.9034693879781574E-5	2.6543591838251472E-5	1.0773575510819715E-4	7.806938775956314E-6
9	3.4350530614207786E-5	4.684163265573789E-5	3.1227755103825256E-5	1.1866546939453598E-4	7.806938775956314E-6
10-11	5.777134694207673E-5	6.167481633005488E-5	3.1227755103825256E-5	1.389635102120224E-4	9.368326531147577E-6
12-13	6.401689796284178E-5	8.041146939235003E-5	3.278914285901652E-5	1.5770016327431755E-4	1.1710408163934473E-5
14-15	7.572730612677626E-5	8.977979592349762E-5	3.8254000002185945E-5	1.7175265307103893E-4	1.7175265307103893E-5
16-17	8.8999102045902E-5	9.992881633224083E-5	4.059608163497284E-5	1.8814722450054717E-4	2.3420816327868946E-5
18-19	1.0773575510819715E-4	1.1085853061857968E-4	4.059608163497284E-5	2.084452653180336E-4	2.6543591838251472E-5
20-21	1.288144898032792E-4	1.1866546939453598E-4	4.059608163497284E-5	2.2249775511475498E-4	2.8104979593442733E-5
22-23	1.5848085715191318E-4	1.3427934694644863E-4	4.684163265573789E-5	2.4279579593224136E-4	2.8104979593442733E-5
24-25	1.9517346939890788E-4	1.5067391837595686E-4	4.840302041092915E-5	2.6543591838251474E-4	3.1227755103825256E-5
26-27	2.5216412246338896E-4	1.6238432653989135E-4	5.46485714316942E-5	2.8261118368961863E-4	3.278914285901652E-5
28-29	3.2320726532459145E-4	1.7175265307103893E-4	5.777134694207673E-5	3.052513061398919E-4	3.5131224491803415E-5
30-31	4.1298706124808903E-4	1.8034028572459088E-4	6.167481633005488E-5	3.294528163453565E-4	3.591191836939905E-5
32-33	5.152579592131168E-4	1.8814722450054717E-4	6.557828571803304E-5	3.4428600001967346E-4	3.8254000002185945E-5
34-35	5.9332734697268E-4	1.982962449092904E-4	6.870106122841557E-5	3.6380334695956427E-4	4.2157469390164103E-5
36-37	7.096507347344289E-4	2.0766457144043797E-4	7.104314286120246E-5	3.825400000218594E-4	4.371885714535536E-5
38-39	8.650088163759597E-4	2.1469081633879866E-4	7.416591837158499E-5	3.9815387757377205E-4	4.606093877814226E-5
40-41	0.001003972326587982	2.2718191838032878E-4	7.650800000437189E-5	4.192326122688541E-4	4.684163265573789E-5
42-43	0.0011936809388437206	2.451378775650283E-4	8.119216326994568E-5	4.418727347191274E-4	4.840302041092915E-5
44-45	0.00142788910212241	2.5606759185136714E-4	8.66570204131151E-5	4.613900816590182E-4	4.918371428852478E-5
46-47	0.0017065968164240504	2.6933938777049286E-4	9.368326531147578E-5	4.848108979868872E-4	5.152579592131168E-5
48-49	0.001986865918480882	2.849532653224055E-4	9.91481224546452E-5	5.027668571715867E-4	5.386787755409857E-5
50-51	0.002334274694010938	2.997864489967225E-4	1.0383228572021899E-4	5.386787755409857E-4	5.6990653064481096E-5
52-53	0.002762094938933344	3.193037959366133E-4	1.116392244961753E-4	5.628802857464504E-4	6.0113428574863625E-5
54-55	0.0032843791430448217	3.3647906124371717E-4	1.1398130612896219E-4	5.964501224830624E-4	6.557828571803304E-5
56-57	0.003884732734915862	3.520929387956298E-4	1.1710408163934472E-4	6.167481633005489E-4	6.557828571803304E-5
58-59	0.004516314081890728	3.723909796131162E-4	1.1788477551694035E-4	6.331427347300571E-4	6.635897959562868E-5
60-61	0.005195517755398928	3.942504081857939E-4	1.2178824490491851E-4	6.620284082010956E-4	7.18238367387981E-5
62-63	0.005966843306463411	4.1298706124808903E-4	1.2334963266010977E-4	6.901333877945382E-4	7.494661224918063E-5
64-65	0.006854492245289645	4.3250440818797984E-4	1.264724081704923E-4	7.158962857551941E-4	7.728869388196752E-5
66-67	0.007834263061672161	4.4967967349508373E-4	1.2803379592568357E-4	7.354136326950849E-4	8.119216326994568E-5
68-69	0.00902325983725031	4.6217077553661385E-4	1.288144898032792E-4	7.580537551453582E-4	8.743771429071072E-5
70-71	0.010416798408758511	4.785653469661221E-4	1.3037587755847046E-4	7.892815102491834E-4	9.134118367868888E-5
72-73	0.011993800041501686	4.887143673748652E-4	1.319372653136617E-4	8.095795510666699E-4	9.368326531147578E-5
74-75	0.013852632164056886	5.074510204371606E-4	1.3818281633442677E-4	8.361231429049213E-4	9.602534694426267E-5
76-77	0.016108056776430665	5.246262857442644E-4	1.413055918448093E-4	8.657895102535553E-4	9.68060408218583E-5
78-79	0.0187819333071957	5.339946122754119E-4	1.4520906123278746E-4	8.946751837245936E-4	1.0070951020983647E-4
80-81	0.022205275960452547	5.496084898273246E-4	1.5223530613114812E-4	9.212187755628452E-4	1.0227089796502773E-4
82-83	0.026577942368865678	5.660030612568328E-4	1.5770016327431755E-4	9.516658367890747E-4	1.0305159184262336E-4
84-85	0.03215756151204165	5.769327755431716E-4	1.6160363266229573E-4	9.774287347497307E-4	1.0305159184262336E-4
86-87	0.03949218049205261	5.863011020743193E-4	1.6238432653989135E-4	0.0010086564898535559	1.0539367347541025E-4
88	0.04639975992101876	5.917659592174887E-4	1.6238432653989135E-4	0.0010367614694469986	1.0773575510819715E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATCGG	14725	0.0	5.617155	94
GATCGGA	11595	0.0	5.552759	94
TCCGATC	6640	0.0	5.0251374	6
>>END_MODULE