FastQCFastQC Report
Wed 25 May 2016
SRR2049540_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049540_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55376546
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA147350.010.04958594
TCCGATC64150.010.0355433
TAGATCG67200.09.37396394
AGATCGG187550.08.99839694
ATCGGAA155500.08.67639394
TTCCGAT84000.08.279622
CCGATCT89950.07.8363554
CGATCTA34650.05.5598845
CTTCCGA145600.05.0690841
TCGGAAG205750.05.003708494
Produced by FastQC (version 0.11.5)